Ecr

Bài báo cáo tập trung nghiên cứu các chuẩn về y khoa như HL7, DICOM để đưa ra giải pháp xây dựng bệnh án điện tử (ECR - Electronic Clinical Record) phục vụ cho việc chẩn đoán y khoa. Từ đó có tác dụng hỗ trợ nghiên cứu lâm sàng, dịch tễ, chất lượng chǎm sóc sức khoẻ, tác dụng của thuốc.

21p benben121 31-10-2014 159 33   Download

The insect ecdysteroid receptor consists of a heterodimer between EcR and the RXR-orthologue, USP. We addressed the question of whether this heterodimer, like all other RXR heterodimers, may be formed in the absence of ligand and whether ligand promotes dimerization. We found that C-terminal protein fragments that comprised the ligand binding, but not the DNA binding domain of EcR and USP and which were equipped with the activation or DNA binding region of GAL4, respectively, exhibit a weak ability to interact spontaneously with each other.

9p system191 01-06-2013 30 3   Download

To develop an ecdysone receptor (EcR)-based inducible gene regulation system, several constructs were prepared by fusing DEF domains of Choristoneura fumiferanaEcR (CfEcR),C. fumiferanaultraspiracle (CfUSP),Mus muscu-lus retinoid X receptor (MmRXR) to either GAL4 DNA binding domain (DBD) or VP16 activation domain. These constructs were tested in mammalian cells to evaluate their ability to transactivate luciferase gene placed under the control of GAL4 response elements and synthetic TATAA promoter. ...

8p tumor12 20-04-2013 40 3   Download

cDNA cloning of ecdysone receptor (EcR) and ultraspiracle (USP) of the coleopteran Colorado potato beetleLeptinotarsa decemlineata(LdEcR and LdUSP) was conducted. Amino-acid sequences of the proteins deduced from cDNA sequences showed striking homology to those of other insects, especially the coleopteran yellow mealworm Tenebrio molitor.

15p fptmusic 11-04-2013 32 3   Download

Nongenomic action of an insect steroid hormone, 20-hy-droxyecdysone (20E), has been implicated in several 20E-dependent events including the programmed cell death of Bombyxanterior silk glands (ASGs), but no information is available for themodeof theaction.Weprovide evidence for a putative membrane receptor located in the plasma mem-brane of the ASGs. Membrane fractions prepared from the ASGs exhibit high binding activity to [ 3 H]ponasterone A (PonA).

9p awards 05-04-2013 45 5   Download

The IAL-PID2 cells derived from imaginal wing discs of the last larval instar of Plodia interpunctellawere responsive to 20-hydroxyecdysone (20E). These imaginal cells respond to 20E by proliferative arrest followed by a morphological differentiation. These 20E-induced late responses were inhibited in presence of juvenile hormone (JH II). From these imaginal wing cells, we have cloned a cDNA sequence encoding aP. interpunctellaecdysone receptor-B1 isoform (PIEcR-B1).

11p awards 05-04-2013 34 3   Download

A pair of nuclear receptors, ecdysone receptor (EcR) and ultraspiracle (USP), heterodimerize and transduce ecdysteroid signals. The EcR and its nonsteroidal ligands are being developed for regulation of transgene expres-sion in humans, animals and plants. In mammalian cells, EcR:USP heterodimers can function in the absence of ligand, but EcR⁄retinoid X receptor (EcR:RXR) heterodimers require the presence of ligand for activa-tion.

12p dell39 27-03-2013 43 4   Download

The steroid hormone 20-hydroxyecdysone (20E) initiates insect molting and metamorphosis through binding with a heterodimer of two nuclear recep-tors, the ecdysone receptor (EcR) and ultraspiracle (USP). Expression of the specific isoforms EcR-A and EcR-B1 governs steroid-induced responses in the developing cells of the silkworm Bombyx mori.

8p inspiron33 25-03-2013 48 5   Download

Regulated expression of transgene is essential in basic research as well as for many therapeutic applications. The main purpose of the present study is to understand the functioning of the ecdysone receptor (EcR)-based gene switch in mammalian cells and to develop improved versions of EcR gene switches.

14p inspiron33 23-03-2013 28 3   Download

In plants, regulation of transgene expression is typically accomplished through the use of inducible promoter systems. The ecdysone receptor (EcR) gene switch is one of the best inducible systems available to regulate transgene expression in plants.

16p media19 06-03-2013 39 3   Download

A set of thirty-two natural and ten semisynthetic ecdysteroids was assayed in murine 3T3 cells across ten different ecdysteroid receptor (EcR) ligand-binding domains derived from nine arthropod species in an engineered gene switch format. Among the ecdysteroids tested, the most biologically wide-spread ecdysteroid, 20-hydroxyecdysone (20E), was moderately and consis-tently potent across the tested EcRs.

25p vinaphone15 28-02-2013 35 3   Download

Insect development is driven by the action of ecdysteroids on morphogenetic processes. The classic ecdysteroid receptor is a protein heterodimer com-posed of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspira-cle (USP), the insect ortholog of retinoid X receptor. The functional properties of EcR and USP vary among insect species, and provide a basis for identifying novel and species-specific insecticidal candidates that disrupt this receptor’s normal activity.

12p viettel02 22-02-2013 36 4   Download

Gene switches have potential applications for the regulation of transgene expression in plants and animals. Recently, we have developed a two-hybrid ecdysone receptor (EcR) gene switch using chimera 9 [CH9, a chi-mera between helices 1–8 ofHomo sapiensretinoid X receptor (HsRXR) and helices 9–12 ofLocusta migratoriaRXR (LmRXR)] as a partner for Choristoneura fumiferanaEcR (CfEcR).

11p viettel02 19-02-2013 32 2   Download

1. Sau khi tìm hiểu các trang web ở trên hãy giải thích thế nào là genome browser ? 2. Mục đích của genome browser ? 3. Cho biết có bao nhiêu genome đã được xác định trình tự ở các genome browser ? 4. Sử dụng UCSC genome browser, hãy cho biết ý nghĩa của : BLAT Gene Sorter Insilico PCR Micribial genomes

6p lovewinter88 11-11-2012 202 20   Download