ELISA assay

Part 2 book "Salmonella - Methods and protocols" includes content: Detecting non typhoid salmonella in humans by enzyme linked immunosorbent assays (ELISAs) - practical and epidemiological aspects; study of the STN protein in salmonella a regulator of membrane composition and integrity, development of a bacterial nanoparticle vaccine,...and other contents.

186p muasambanhan01 13-12-2023 8 4   Download

Part 1 book "ELISA - Methods and protocols" includes content: Flow through assay for detection of antibodies using protein a colloidal gold conjugate as a probe; multiplexed microsphere suspension array based immunoassays; lateral flow immunoassay, immuno pcr assay for sensitive detection of proteins in real time; tyramide signal amplification for immunofluorescent enhancement;.... and other contents.

116p oursky05 20-09-2023 3 2   Download

Outbreaks of the Southern rice black-streaked dwarf virus (SRBSDV) have caused significant losses in many rice-growing areas in Vietnam, especially in both North and Central Vietnam in recent years. To detect the virus, traditional reverse transcription polymerase chain reaction (RT-PCR) methodology and immunoassays are currently employed. RT-PCR is accurate but requires expensive chemicals and instruments, as well as complex procedures that limit its applicability for field tests.

8p viengels 25-08-2023 5 2   Download

The 2-methylpyridine, 2-diethylaminoethyl, and isopentyl linked a series of symmetric and unsymmetric benzimidazolium salts 2a-e were prepared and used in the synthesis of silver-N-heterocyclic carbene (NHC) complexes (3a-e). We examined the effect of the structural difference of complexes on anticancer activity via different arenes and metal centers. Antiproliferative activity of 5a-h and 3a was tested against human cervix adenocarcinoma (HeLa) and rat glioblastoma (C6) cell lines by ELISA assay.

26p lyhuyenthu 31-01-2023 4 2   Download

Trong nghiên cứu này, kháng thể đa dòng kháng đặc hiệu SRBSDV được tạo ra từ kháng nguyên là đoạn peptide (PASTTDVTHYGGY) giàu tính kháng nguyên có nguồn gốc từ protein vỏ P10 của SRBSDV và bảo thủ ở các nòi SRBSDV của Việt Nam. Kháng thể tinh sạch thu được liên kết đặc hiệu với protein P10 ở nồng độ kháng thể pha loãng 1:40000 và phát hiện được sự có mặt của SRBSDV trong mẫu lúa nhiễm bệnh lùn sọc đen bằng kỹ thuật Dot-ELISA (Dot enzyme-linked immunosorbent assay).

7p viellenkullman 13-05-2022 21 1   Download

Leptospirosis is a widespread zoonosis and has been recognized as a re-emerging infectious disease in humans and dogs, but prevalence of Leptospira shedding in dogs in Thailand is unknown. The aim of this study was to determine urinary shedding of Leptospira in dogs in Thailand, to evaluate antibody prevalence by microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA), and to assess risk factors for Leptospira infection.

13p vigalileogalilei 28-02-2022 8 1   Download

Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. Vaccination plays a vital role in controlling the disease. The efficacy of vaccination in chicken flocks can be evaluated by detecting neutralizing antibodies with the neutralization test. However there are no simple and rapid methods for detecting the neutralizing antibodies.

7p vidarwin 23-02-2022 9 1   Download

This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the detection of antibodies against MAP. Four diagnostic applied tests were evaluated including Indigenous plate-ELISA (IP-ELISA), Mt-ELISA, fecal Polymerase Chain Reaction (f-PCR) and fecal culture (FC).

9p vidarwin 22-02-2022 9 1   Download

Novel 1-substituted 3-(p-isopropylphenyl)-5-phenylformazans (3a–g) were synthesized and characterized by elemental analysis, 1H NMR, and FT-IR techniques and UV-visible spectroscopy. Antiproliferative activities of 3a–g against HeLa and C6 cells were determined using the BrdU cell proliferation ELISA assay. 5-Florouracil was used as the positive control. The effects of substituents (–H, –CH3, and –I) and their positions (ortho, meta, and para) on the antiproliferative activities were evaluated.

9p tudichquannguyet 29-11-2021 8 1   Download

In this research, a new series of (E) -3-(4-substitutedphenyl)-1-(4’-morpholinophenyl)prop-2-en-1-one derivatives 1–7 was synthesized, aiming to develop effective antiproliferative agents. The antiproliferative activities of compounds 1–7 were examined against HeLa and C6 cell lines at eight different concentrations using the BrdU ELISA assay. The activity results were compared with reference anticancer drug 5-fluorouracil (5-FU). Compound 1 had almost the same antiproliferative activity as 5-FU.

19p tudichquannguyet 29-11-2021 6 0   Download

This case-control study was conducted on 67 patients with PCa and 40 control subjects. The expression levels of cellular and viral factors involved in inflammation, tumor progression, and metastasis were quantified, using the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRTPCR) assay.

19p vimahuateng 26-11-2021 7 1   Download

The abuse of antibiotics leads to antibiotic resistance which may seriously threaten human and animal welfare, and growing levels of antibiotics or antibiotic-resistant bacteria in the environment increase the numbers of drug-resistant infection outbreaks. Therefore, many detection methods have been being developed to quickly assess antibiotic content and its residues in foods.

10p spiritedaway36 25-11-2021 7 0   Download

In the present study, a fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase real time PCR assay (RT-qPCR)was evaluated parallel to conventional RT-PCR and antigen capture ELISA to detect Classical swine fever virus (CSFV) in the pre-clinical phase of the disease.

10p chauchaungayxua12 29-07-2021 18 2   Download

During the pathological destruction of lung tissue, neutrophil elastase (NE) degrades elastin, one of the major constituents of lung parenchyma. However there are no non-invasive methods to quantify NE degradation of elastin. We selected specific elastin fragments generated by NE for antibody generation and developed an ELISA assay (EL-NE) for the quantification of NE-degraded elastin.

7p vimontana2711 05-04-2021 11 3   Download

The present study aimed to investigate the role of serum midkine level in rheumatoid arthritis (RA) patients and to analyze the relationship between the serum MK level and disease activity. Blood samples were collected from 60 patients with rheumatoid arthritis and twenty healthy individuals served as control group. Serum level of Midkine was measured by Enzyme Linked Immunosorbent Assay (ELISA).

8p chauchaungayxua11 23-03-2021 16 2   Download

The study was conducted in Department of Microbiology RIMS, Raichur. Blood samples were collected from the patients attending orthopedic OPD of RIMS teaching hospital with complaints of fever, joint pain and backache. RBPT, MAT and ELISA IgM and IgG were carried out on serum samples.

9p gaocaolon9 22-12-2020 14 1   Download

Recombinant human CD45RO antigen was expressed from E. coli BL21 (DE3), purified and analyzed by SDS-PAGE and Western blotting. The purified CD45RO antigen was used to immunize Balb/c mice. Spleen cells from immunized mouse were collected and fused with P3X63Ag8.653 myeloma cells to form hybridoma. Anti-CD45 antibody-secreting capacity of hybridoma clones was evaluated by ELISA assay. Anti-CD45 MAb from the culture supernatant of the chosen hybridoma clone was purified by affinity chromatography. The MAb was characterized the biochemical characteristics and biological activity.

8p trinhthamhodang9 04-12-2020 12 2   Download

The σB protein of avian reovirus (ARV) is diagnostically important protein for development of an ARV-specific enzyme-linked immunosorbent assay (ELISA) and for which production of an antigenic recombinant σB protein in abundance is a prerequisite. In the present study, we successfully employed the prokaryotic system for expression of antigenic σB protein of ARV.

8p angicungduoc8 07-11-2020 17 2   Download

Tài liệu giới thiệu tới bạn đọc các kỹ thuật ELISA (Enzym Link Immunosorbent Assay), kỹ thuật hóa mô miễn dịch (Imnumohistochemistry AssayX kỹ thuật nhân bản gen (Polymerase Chaine Reactìon - PCR), kỹ thuật lai phân từ (Hybridization)... dùng trong chẩn đoán bệnh động vật. Mời các bạn cùng tham khảo.

143p koxih_kothogmih7 29-09-2020 36 2   Download

To determine the diagnostic and prognostic capability of urinary and tumoral syndecan-1 (SDC-1) levels in patients with cancer of the urinary bladder. Methods: SDC-1 levels were quantitated by enzyme-linked immunosorbent assay (ELISA) in 308 subjects (102 cancer subjects and 206 non-cancer subjects) to assess its diagnostic capabilities in voided urine.

7p virose2711 25-09-2020 14 0   Download