Escherichia coli inactivation
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Pulsed light is an emerging non thermal processing technology used for food decontamination. A laboratory scale pulsed light treatment chamber was designed and fabricated. In this study, tender coconut water was exposed to pulsed light to inactivate the E. coli using different process parameter such as juice layer depth (5, 10, 15 and 20mm), shelf height (5, 10 and 15cm) and number of pulses (60, 120, 180 and 240 flashes) corresponding to an fluency of 4.8, 9.6, 14.4 and 19.2J/cm2 .
9p chauchaungayxua8 03-10-2020 12 0 Download
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Hence, it is important to study the inactivation mechanism of any novel processes before stepping into the market. E. coli inoculated distilled water sample is subjected to ultrasound treatment at 20 KHz frequency with single amplitude of 60 µm for 15 minutes. TEM images obtained before and after sonicated samples is an evident to understand the lethality of E. coli bacterium by cavitation effect of ultrasound.
5p chauchaungayxua8 03-10-2020 9 1 Download
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This study presents results for the use of UV radiation and a liquid-film-forming device (LFFD) for disinfection of water. Escherichia coli was used as a model microorganism for examining the bactericidal performance of UV. Bacterial inactivation was conducted in a disinfection apparatus with various conditions of UV dosages, air flow rates, and initial bacterial concentrations. Combined UV/LFFD treatments resulted in a greater inactivation efficiency than those for the UV treatment alone. Combined treatment with UV (UV dosage = 3.020×10-20 kJ/m2 , initial bacterial concentration = 1.
8p angicungduoc5 13-06-2020 11 1 Download
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One of the most important properties of the protease enzyme is to degrade the biofilm because removal of biofilms is very difficult. In industrial settings, both the inactivation and removal of biofilms are of huge concern. If only disinfection without the removal of attached biofilms occurs, the inactivated biofilm cells may provide an ideal environment for further adhesion and growth, resulting in a complex matrix. Microbial resistance to biocides and their negative environmental impact are the main reasons for finding alternative biofilm control strategies.
10p chauchaungayxua5 08-05-2020 10 2 Download
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Ricin A chain (RTA) and Pokeweed antiviral proteins (PAPs) are plant-derived N-glycosidase ribosomalinactivating proteins (RIPs) isolated from Ricinus communis and Phytolacca Americana respectively.
11p vihamax2711 21-04-2020 14 1 Download
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To examine functions of two small heat shock proteins of Escherichia coli, IbpA and IbpB, we constructed His–IbpA and His–IbpB, in which a polyhistidine tag was fused to the N-terminals. Both purified His–IbpA and His–IbpB formed multimers, which have molecular masses of about 2.0– 3.0 MDa and consist of about 100–150 subunits.
11p system191 01-06-2013 35 2 Download
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Four substrate analogs, 4-(2-naphthyloxy)-2-butyn-1-amine (1), 1,4-diamino-2-chloro-2-butene (2), 1,6-diamino-2,4hexadiyne (3), and 2-chloro-5-phthalimidopentylamine (4) have been tested as inhibitors against mammalian, plant, bacterial, and fungal copper-containing amine oxidases: bovine plasma amine oxidase (BPAO), equine plasma amine oxidase (EPAO), pea seedling amine oxidase (PSAO), Arthrobacter globiformis amine oxidase (AGAO), Escherichia coli amine oxidase (ECAO), and Pichia pastoris lysyl oxidase (PPLO).
14p research12 01-06-2013 40 4 Download
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Thirteen glucose analogues bearing electrophilic groups were synthesized (five of themfor the first time) and screened as inhibitors of the glucose transporter (EII Glc )of the Escherichia coli phosphoenolpyruvate–sugar phospho-transferase system (PTS). 2¢,3¢-Epoxypropyl b-D-glucopyr-anoside (3a) is an inhibitor and also a pseudosubstrate. Five analogues are inhibitorsofnonvectorialGlcphosphorylation by EII Glc but not pseudosubstrates.
12p tumor12 22-04-2013 47 2 Download
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Cells that have lost the ability to grow in culture could be defined operationally as either alive or dead depending on the method used to determine cell viability. As a conse-quence, the interpretation of the state ofnonculturablecells is often ambiguous.Escherichia coliK12 cells inactivated by UV-irradiation with a low (UV1) and a high (UV2) dose wereusedas amodel of nonculturable cells. Cells inactivated by the UV1 dose lostculturability but they were not lysed andmaintained the capacity to respond to nutrient addition by protein synthesis and cell wall synthesis. ...
7p fptmusic 16-04-2013 21 1 Download
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The active form of the oxygen sensor fumarate nitrate reductase regulator (FNR) of Escherichia colicontains a [4Fe-4S] cluster which is converted to a [2Fe-2S] cluster after reaction with air, resulting in inactivation of FNR. Reaction of reconstituted [4Fe-4S]ÆFNR with air resulted within 5 min in conversion to apoFNR. The rate was comparable to the rate known for [4Fe-4S]ÆFNR⁄[2Fe-2S]ÆFNR cluster conversion, suggesting that apoFNR is a product of [2Fe-2S]ÆFNR decomposition and a final form of air-inacti-vated FNR in vitro. ...
10p fptmusic 11-04-2013 31 1 Download
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Pulchellin is a type 2 ribosome-inactivating protein isolated from seeds of the Abrus pulchellus tenuiflorusplant. This study aims to obtain active and homogeneous protein for structural and biological studies that will clarify the functional aspects of this toxin. The DNA fragment encoding pulchellin A-chain was cloned and inserted into pGEX-5X to express the recombinant pulchellin A-chain (rPAC) as a fusion protein in Escherichia coli. The deduced amino acid sequence analyses of the rPAC presented a high sequential identity ( 86%) with the A-chain of abrin-c. ...
0p awards 05-04-2013 38 1 Download
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Exoribonuclease II (RNase II), encoded by thernb gene, is a ubiquitous enzyme that is responsible for 90% of the hydrolytic activity in Escherichia colicrude extracts. The E. colistrain SK4803, carrying the mutant allelernb296, has been widely used in the study of the role of RNase II. We determined the DNA sequence ofrnb296 and cloned this mutant gene in an expression vector. Only a point mutation in the coding sequence of the gene was detected, which results in the single substitution of aspartate 209 for asparagine....
0p awards 05-04-2013 41 1 Download
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Escherichia colifatty acid cyclopropane synthase (CFAS) was overproduced and purified as a His6 -tagged protein. This recombinant enzyme is as active as the native enzyme with aKmof 90lMforS-AdoMet and a specific activity of 5·10 )2 lmolÆmin )1 Æmg )1 . The enzyme is devoid of organic or metal cofactors and is unable to catalyze the wash-out of themethyl protons ofS-AdoMet to the solvent, data that do not support the ylidemechanism. Inactivationof the enzyme by 5,5¢-dithiobis-(2-nitrobenzoic acid) (DTNB), a pseudo first-order process with a rate constant of 1.
10p awards 05-04-2013 30 1 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Escherichia coli inactivation kinetics in anaerobic digestion of dairy manure under moderate, mesophilic and thermophilic temperatures
10p dauphong12 08-02-2012 57 5 Download