Expression vector

Glioblastoma (GBM) can be divided into subtypes according to their genomic features, includ‑ ing Proneural (PN), Neural (NE), Classical (CL) and Mesenchymal (ME). However, it is a difficult task to unify various genomic expression profiles which were standardized with various procedures from different studies and to manually classify a given GBM sample into a subtype.

13p vielonmusk 21-01-2022 16 1   Download

In this study, the DNA fragment encoding for the C-terminus domain of the AopB from Aeromonas hydrophila AH-1 was cloned into pET-M expression vector and expressed in Escherichia coli BL21 (DE3) host cells. The recombinant AopB-C-terminus domain was successfully purified using immobilized nickel affinity chromatography as a soluble form. Crosslinking analysis among AopB-C-terminus molecules in solution showed that this domain existed as a mixture of tetramer, trimer, dimer, and monomer forms.

6p guitaracoustic02 08-12-2021 18 3   Download

Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains.

10p thiencuuchu 27-11-2021 10 1   Download

In this study, the Mgra16281 gene (ID: MK322955.1) enco ding an effector with the unknown function was cloned from the rice ro ot-knot nematode Meloidogyne graminicola isolated in Long An province. To knock-down the expression of this gene, an artificial microRNA was synthesized based on the Osa-MIR528 precursor and inserted into an expression vector.

9p larachdumlanat129 19-01-2021 14 1   Download

In this study, we selected a novel gene encoding protease inhibitor from metagenome of marine sponge QT collected in Quang Tri and expressed in the Escherichia coli (E. coli) strain BL21(DE3) for finding and mining novel protease inhibitor.

8p shiwo_ding7 05-06-2019 34 1   Download

High-Level Recombinant Protein Production in CHO Cells Using Lentiviral Vectors and the Cumate Gene-Switch developed  an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant  proteins. It is based on lentiviral vectors (LVs) for stable transduction coupled with the cumate gene-switch for inducible and efficient gene expression.

13p thuyancn 01-06-2015 35 3   Download

A novel vector platform for vitamin H-inducible transgene expression in mammalian cells have therefore designed a strategy to convert antibiotic-responsive transcription factors into gene regulation systems responsive to non-toxic biotin, also known as vitamin H.

9p thuyancn 01-06-2015 42 4   Download

Trypanosoma brucei is the cause of the diseases known as sleeping sickness in humans (T. brucei ssp. gambiense and ssp. rhodesiense) and ngana in domestic animals (T. brucei brucei) in Africa. Procyclic trypomastigotes, the tsetse vector stage, express a surface-bound trans-sialidase that transfers sialic acid to the glycosylphosphatidylinositol anchor of procyclin, a surface glycoprotein covering the parasite surface. Trans-sialidase is a unique enzyme expressed by a few trypanosomatids that allows them to scavenge sialic acid from sialylated compounds present in the infected host. ...

10p system191 01-06-2013 44 4   Download

Mucopolysaccharidosis type I (MPS I) results from a deficiency in the enzyme a-L-iduronidase (IDUA), and is characterized by skeletal abnormalities, hepatosplenomegaly and neurological dysfunction. In this study, we used a late generation lentiviral vector to evaluate the utility of this vector system for the transfer and expression of the human IDUA cDNA in MPS I fibroblasts. We observed that the level of enzyme expression in transduced cells was 1.5-fold the level found in normal cells; the expression persisted for at least two months....

8p system191 01-06-2013 31 5   Download

A clone encoding farnesyl diphosphate synthase (FPPS) was obtained by PCR from a cDNA library made from young leaves of Artemisia annua. A cDNA clone encoding the tobacco epi-aristolochene synthase (eAS) was kindly supplied by J. Chappell (University of Kentucky, Lexington, KY, USA). Two fusions were constructed, i.e. FPPS/ eAS and eAS/FPPS. The stop codon of the N-terminal enzyme was removed and replaced by a short peptide (Gly-SerGly) to introduce a linker between the two ORFs.

8p research12 01-06-2013 36 3   Download

A gene having high sequence homology (45–49%) with the glycerol-1-phosphate dehydrogenase gene from Methanobacterium thermoautotrophicum was cloned from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1 (JCM 9820). This gene expressed in Escherichia coli with the pET vector system consists of 1113 nucleotides with an ATG initiation codon and a TAG termination codon. The molecular mass of the purified enzyme was estimated to be 38 kDa by SDS/PAGE and 72.4 kDa by gel column chromatography, indicating presence as a dimer....

8p research12 01-06-2013 27 4   Download

Random PCR mutagenesis was applied to theThermus thermophilus xylA gene encoding xylose isomerase. Three cold-adapted mutants were isolated with the following amino-acid substitutions: E372G, V379A (M-1021), E372G, F163L (M-1024) and E372G (M-1026). The wild-type and mutated xylAgenes were cloned and expressed in Escherichia coliHB101 using the vector pGEMÒ-T Easy, and their physicochemical and catalytic properties were determined.

7p research12 29-04-2013 54 3   Download

The presence of extraN- andC- terminal residues can play a major role in the stability, solubility and yield of recombi-nant proteins. Pfg27 is a 27Ksoluble protein that is essential for sexual development in Plasmodium falciparum.It was over-expressed using the pMAL-p2 vector as a fusion pro-teinwith themaltosebindingprotein. Sixdifferent constructs were made and each of the fusion proteins were expressed and purified.

5p tumor12 22-04-2013 34 2   Download

The availability of a system for the functional expression of genes coding formolybdenumhydroxylases is a prerequisite for the construction of enzyme variants bymutagenesis. For the expression cloning of quinoline 2-oxidoreductase (Qor) fromPseudomonas putida86 – that contains the molybdo-pterin cytosine dinucleotide molybdenum cofactor (Mo-MCD), two distinct [2Fe)2S] clusters and FAD – the qorMSLgenes were inserted into the broad host range vector, pJB653, generating pUF1.P. putidaKT2440 and P. putida86-1Dqor were used as recipients for pUF1....

11p tumor12 20-04-2013 56 5   Download

Seven alleles of the chicken melanocortin (MC) 1 receptor were cloned into expression vectors, expressed in mam-malian cells and pharmacologicallycharacterized. Four of the clones e +R ,e +B&D ,e wh /e y ,E Rfayoumi gave receptors to which melanocortin stimulating hormone (a-MSH) and NDP-MSH bound with similar IC50values and responded toa-MSHbyincreasing intracellular cAMP levels in a dose-dependent manner.

9p tumor12 20-04-2013 31 3   Download

A thermostable enzyme from the hyperthermophilic sul-phate-reducing archaeon, Archaeoglobus fulgidus,was expressed and characterized on the assumption that it is homologous to exonuclease III from Escherichia coli. Sequence similaritydatabase searcheswere performedbased on the amino acid sequence of exonuclease III. The 774bp longgenewas isolated froma culture sample andcloned into different vectors.

12p fptmusic 16-04-2013 27 3   Download

The herpes simplex virus thymidine kinase⁄ganciclovir (HSV-tk⁄GCV) sys-tem that selectively depletes cells expressing HSV-tk upon treatment with GCV has provided a valuable tool for developing a new animal model expressing the desired tissue damage. In this paper, an HSV-tk vector with an albumin promoter⁄enhancer was constructed. Based on the favourable killing effect on Hep-G2 cells by the recombinant construct, the HSV-tk transgenic mouse strains were developed.

9p awards 06-04-2013 52 2   Download

Exoribonuclease II (RNase II), encoded by thernb gene, is a ubiquitous enzyme that is responsible for 90% of the hydrolytic activity in Escherichia colicrude extracts. The E. colistrain SK4803, carrying the mutant allelernb296, has been widely used in the study of the role of RNase II. We determined the DNA sequence ofrnb296 and cloned this mutant gene in an expression vector. Only a point mutation in the coding sequence of the gene was detected, which results in the single substitution of aspartate 209 for asparagine....

0p awards 05-04-2013 42 2   Download

In the nonmevalonate pathway of isoprenoid biosynthesis, the conversion of 2C-methyl-D-erythritol 4-phosphate into its cyclic diphosphate proceeds via nucleotidyl intermediates and is catalyzed by the products of theispD,ispE andispF genes. An open reading frame of Campylobacter jejuni with similarity to theispDandispFgenes ofEscherichia coli was cloned into an expression vector directing the formation of a 42 kDa protein in a recombinantE. colistrain.

8p awards 05-04-2013 39 5   Download

The transcription factor nuclear factorjB(NF-jB) is an activator of multiple cytokines, chemokines and adhesion molecules, which are important in inflammatory diseases such as asthma, and is consequently considered as an attractive therapeutic target. In the present study, a con-stitutively active dominant version of IjBa,IjBaDN, was introduced into A549 pulmonary cells by adenovirus-mediated delivery. The dominant IjB, but not a null viral vector, prevented the induction of NF-jB-dependent transcription by both tumor necrosis factora(TNFa)and interleukin-1b(IL-1b). ...

7p dell39 03-04-2013 34 4   Download