Báo cáo khoa học: Biosynthesis of isoprenoids A bifunctional IspDF enzyme fromCampylobacter jejuni
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In the nonmevalonate pathway of isoprenoid biosynthesis, the conversion of 2C-methyl-D-erythritol 4-phosphate into its cyclic diphosphate proceeds via nucleotidyl intermediates and is catalyzed by the products of theispD,ispE andispF genes. An open reading frame of Campylobacter jejuni with similarity to theispDandispFgenes ofEscherichia coli was cloned into an expression vector directing the formation of a 42 kDa protein in a recombinantE. colistrain.
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