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Fluorescence activated cell sorting
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Ebook "Hematopoietic stem cell biology" discoveries were made possible in part by advancements in technology. For example, recent availability of user friendly fluorescence activated cell sorting (FACS) machines and monoclonal an- bodies with a variety of fluorescent labels has allowed more scientists to sort and analyze rare populations in the bone marrow, such as HSCs. All classes of hematopoietic cells are derived from HSCs.
260p
manmanthanhla0201
27-02-2024
2
1
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Ebook "T-cell differentiation: Methods and protocols" provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails,...
272p
lucchinguyen
28-12-2023
6
2
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While we are taught to never judge a book by its cover, covers may actually be revealing. In the case of a cell, the surface proteins on its “cover” are unique to particular cell types: for example, CD3 for T cells and CD19 for B cells. With such markers in hand, populations of cells can be classified into the cell types they contain, in particular using fluorescence-activated cell sorting (FACS) analysis with a panel of antibodies.
3p
vialfrednobel
29-01-2022
14
0
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The potential DNA demethylating properties of the analyzed compounds were tested by MethylationSensitive High Resolution Melting (MS-HRM). Cell cycle distribution was tested using Fluorescence-Activated Cell Sorting (FACS), whereas apoptosis was analyzed using FITC Annexin V/propidium iodide double staining assay and Western blot.
15p
lazzaro
30-12-2021
7
0
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E2F6 is a member of the E2F family of transcription factors involved in regulation of a wide variety of genes through both activation and repression. E2F6 has been reported as overexpressed in breast cancers but whether or not this is important for tumor development is unclear. We first checked E2F6 expression in tumor cDNAs and the protein level in a range of breast cancer cell lines.
13p
thiencuuchu
27-11-2021
6
0
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Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a celltype specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling.
16p
visilicon2711
20-08-2021
11
1
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Bovine IL28B protein expressed and purified from Pichia pastoris was used for the study. IL28B protein coated with Poly (lactic-coglycolic acid) (PLG) microsphere which was prepared by double emulsion-solvent evaporation technique (Feczko et al., 2011) was used for the study.
5p
cothumenhmong7
08-09-2020
10
1
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Breast cancer (BC) is highly heterogeneous with ~ 60–70% of estrogen receptor positive BC patient’s response to anti-hormone therapy. Estrogen receptors (ERs) play an important role in breast cancer progression and treatment.
15p
vibaku2711
22-07-2020
9
1
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Prostate cancer is the most-diagnosed non-skin cancer among males in the US, and the second leading cause of cancer-related death. Current methods of treatment and diagnosis are not specific for the disease.
11p
viuchiha2711
21-04-2020
8
0
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CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential.
12p
vihamax2711
21-04-2020
11
0
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Hair follicle is an ideal system for studying the biology and regulation of adult stem cells. Multipotent stemness of the canine hair follicle stem cells (cHFSCs) were detected by immunostaining the cells with positive HFSC surface markers like Anti-cytokeratin-15 (CK15) and CD200. In this study, canine hair follicular cells were cultured in Williams’E media supplemented with growth factors.
6p
chauchaungayxua4
22-03-2020
12
2
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In order to evaluate the ability of the cell-penetrating a-helical amphipathic model peptide KLALKLALKALK AALKLA-NH2 (MAP) to deliver peptide nucleic acids (PNAs) into mammalian cells, MAP was covalently linked to the 12-mer PNA 5¢-GGAGCAGGAAAG-3¢ directed against themRNAof the nociceptin/orphaninFQ receptor. The cellular uptake of both the naked PNA and its MAP-conjugate was studied by means of capillary electrophoresis combined with laser-induced fluorescence detection, confo-cal laser scanningmicroscopyandfluorescence-activatedcell sorting. ...
0p
awards
05-04-2013
29
1
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