Fluorescent proteins GFP

Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, Green Fluorescent Protein (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of a synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli).

10p vimulcahy 18-09-2023 4 3   Download

To characterize the cellular stress by n-alkanols with different alkyl chain lengths in Escherichia coli, we investigated how n-alkanols damage cell envelope permeability and whether they enhance the promoter activity of the envelope stress response regulator, σE , by using variants of green fluorescent protein (GFP)

14p vicaptainmarvel 21-04-2023 5 2   Download

The aminated-silica nanoparticles with a positive zeta potential interact with GFP having a negative zeta potential via ionic force. GFP immobilized by the aminated silica nanoparticles greatly enhances (ca. 2 times) the bioluminescence intensity compared to free GFP. The lipase/aminated-silica nanoparticles catalyzed the transesterification reaction of 1-hexanol and ethyl acetate.

7p spiritedaway36 28-11-2021 6 0   Download

In the present study, attempts were made to produce biologically active recombinant mouse hephaestin as a secretory form tagged with green fluorescent protein (GFP), Hpsec-GFP. Plasmid expressing Hpsec-GFP was constructed and transfected into COS and CHO cells. The GFP aided the monitoring expression in real time to select the best conditions to maximise expression and provided a tag for purifying and analysing Hpsec-GFP. The protein had detectable oxidase activity as shown by in-gel and solution-based assays.

13p thiencuuchu 27-11-2021 8 1   Download

The ecdysone inducible system was generated for checking the the toxicity of the target gene which was transfected into the cells. The retinoid X receptor (RXR) was transfected into HEK293FT to generate an inducible stable cell line pVgRXR-HEK293FT. For checking the function, the cells transfected with pIND-GFP and induced by 1µM ponasteron A for GFP expression. The fluorescence signals were detected in the transfected and induced cells informed the success of experiment.

7p trinhthamhodang1218 18-03-2021 11 1   Download

The tauGFP reporter fusion protein is produced nearly ubiquitously by the TgTP6.3 transgene in TP6.3 mice and its localisation to microtubules offers some advantages over soluble GFP as a lineage marker. However, TgTP6.3Tg/Tg homozygotes are not viable and TgTP6.3Tg/− hemizygotes are smaller than wild-type.

16p viwyoming2711 16-12-2020 10 1   Download

Bài viết trình bày ứng dụng kỹ thuật miễn dịch huỳnh quang kết hợp phân tích hình ảnh nội hàm cao để phát hiện và định lượng sự chuyển vị của tiểu đơn vị NF-κB p65 liên kết protein huỳnh quang xanh (green fluorescent protein, GFP) kích hoạt bởi cytokine trên dòng tế bào ung thư cổ tử cung HeLa.

9p kethamoi7 15-08-2020 86 4   Download

To determine the titer of baculovirus stock accurately based on green fluorescent protein (GFP) reporter, a recombinant baculovirus encoding FLAG-tagged protein and GFP reporter as bicistronic messenger was constructed. The bicistronic messenger RNA was transcribed using polyhedrin promoter and translated in infected Sf21 insect cells. The GFP reporter gene was expressed incap independent manner utilizing internal ribosome entry site (IRES) which helped in titration of baculovirus using end point titration method.

9p quenchua6 15-06-2020 10 1   Download

The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms. Here, the CRISPR/ Cas9 system is shown to be effective in soybean by knocking-out a green fluorescent protein (GFP) transgene and modifying nine endogenous loci.

10p viuchiha2711 21-04-2020 12 1   Download

A superior Green Fluorescent Protein (GFP) mutant, known as superfolder GFP (sfGFP), is more soluble, faster folding, and is the brightest of the known GFP mutants. This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purification in Tetrahymena thermophila.

9p viuchiha2711 21-04-2020 10 0   Download

The present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (GA) C15:1 monomer using green fluorescent protein (GFP)-labeled bacteria strains.

12p vihamax2711 21-04-2020 13 0   Download

The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics.

8p vihamax2711 21-04-2020 13 1   Download

The Microvirga zambiensis strain WSM 3693 is an endophytic strain isolated from saline area of kutch, Gujarat. The green fluorescent protein (GFP) was used to mark this endophyte in order to visualize and analyze the colonization processes in vivo. After inoculation for 2 to 15 d, it was visualized in cotton tissue using fluorescence microscopy.

5p cothumenhmong1 11-12-2019 10 0   Download

In this study, we evaluated the application of green fluorescent protein (GFP) from the jellyfish Aequorea victoria, for efficient selection of possible transgenic and non-mosaic mouse embryos after microinjection.

9p caplock2711 19-02-2019 27 0   Download

The recombinant vector was screened by PCR method, sequenced and aligned with designed sequences. The in-frame vector was then introduced into E. coli BL21(DE3) for expression by inducing with 0.5mM IPTG. Fusion protein was purified using Ni-affinity chromatography. The results showed that the fused genes were in-frame cloned and completely matched with the designed sequences. SDS-PAGE and Western blot analyses showed Co1-GFP protein expressed in soluble form and could be purified at one-step elution of 500mM imidazole.

6p nguyenhong1235 28-11-2018 29 0   Download

Luận văn nghiên cứu với mục tiêu nhằm mục đích làm tiền đề cho các nghiên cứu tiếp theo luận văn tiến hành nghiên cứu xây dựng qui trình thu nhận tế bào tiền thân nội mô từ máu cuống rốn người và thiết lập tế bào tiền thân nội mô từ máu cuống rốn người biểu hiện gen gfp để theo dõi đánh giá chức năng của chúng về khả năng sửa chữa nội mạch trong mô hình chuột thiếu máu chi cục bộ và thiếu máu cơ tim cục bộ. Để biết rõ hơn về nội dung chi tiết, mời các bạn cùng tham khảo.

78p dangthingocthuy96 09-01-2017 87 12   Download

Tham khảo luận văn - đề án 'chuyển gen gfp (green fluorescent protein) vào tế bào vi khuẩn pseudomonas fluorescens bằng phương pháp tiếp hợp ba thành phần', luận văn - báo cáo, khoa học tự nhiên phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

81p canhchuon_1 20-06-2013 137 27   Download

Wehave identifiedanovelmammaliangene, termednicolin1 gene (NICN1), that is present in human, dog and mouse, whereas it is absent from the available genome sequences of nonmammalian organisms. TheNICN1gene consists of six exons and spans about 6 kb of genomic DNA. It encodes a 213 amino acid protein that does not belong to any known protein family. Experiments using green fluorescent protein (GFP)-taggednicolin1 fusionproteins indicate that nicolin1 is a nuclear protein.

6p tumor12 22-04-2013 47 1   Download

Pigments homologous to the green fluorescent protein (GFP) contribute up to 14% of the soluble protein content of many anthozoans. Maintenance of such high tissue levels poses a severe energetic penalty to the animals if protein turnover is fast. To address this as yet unexplored issue, we estab-lished that the irreversible green-to-red conversion of the GFP-like pig-

10p galaxyss3 21-03-2013 42 3   Download

Fluorescence recovery after photobleaching (FRAP) and related techniques using green fluorescent protein (GFP)-tagged proteins are widely used to study the subcellular trafficking of proteins. It was concluded from these experiments that the cytokine-induced nuclear import of tyrosine-phos-phorylated (activated) signal transducer and activator of transcription 1 (STAT1) was rapid, while the constitutive shuttling of unphosphorylated STAT1 was determined to be inefficient.

12p galaxyss3 21-03-2013 34 2   Download