Gamma Co-60 irradiation

The present experiment was conducted to standardize the protocol for in vitro mutagenesis of gladiolus hybridus varieties ‘Punjab Glance’ and ‘Sylvia’. The nodal buds were irradiated with different doses of gamma rays (Co60) 0, 5, 8, 10 and 12 Gy. Irradiated explants were then cultured aseptically on MS basal medium supplemented with 5 mg/l (BAP) + 2mg/l (IBA) and agar-agar to induce shoot and root proliferation.

11p gaocaolon9 22-12-2020 9 3   Download

Three rice genotypes of NPT4, NPT5, and QP-5 were successfully selected through mutation breeding using Co60 gamma rays with 250-300 Gy. Of the three lines, two genotypes, NPT4 and NPT5, obtained high yields of 8.9- 9.2 tonnes/ha, had growth duration of 103-110 days in the season of autumn and 130-135 days in the spring season. They exhibited dark green leaves, which were found to be adapted to intensification.

5p caygaocaolon1 13-11-2019 8 0   Download