Gene delivery system

Ebook "Functionalized conjugated polyelectrolytes: Design and biomedical applications" presents a comprehensive review of these polyelectrolytes and their biomedical applications. Basic aspects like molecular design and optoelectronic properties are covered in the first chapter. Emphasis is placed on the various applications including sensing (chemical and biological), disease diagnosis, cell imaging, drug/gene delivery and disease treatment.

91p coduathanh1122 27-03-2024 2 1   Download

Ebook "Retinal gene therapy: Methods and protocols" describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies.

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The aim of this study was to develop hepatocyte-targeting non-viral polymeric nono-carriers for gene delivery. Chitosan was selected as the main polymer. An asialoglycoprotein receptor recognized sugar, galactose, was introduced.

8p vihermione 06-01-2023 8 2   Download

Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP®R. They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.

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This study aims to establish promising and simple MNP-based protocol for gene delivery initially. Specifically, we aim to evaluate whether sodium chloride (NaCl) could enhance gene transfer with naked plasmid DNA without the need to combine liposomes or polymersomes.

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. In this study, the VEGF121 gene was cloned and expressed in a prokaryotic expression system (Escherichia coli). The recombinant VEGF121 was encapsulated with PLA microparticles and studied in vitro and ex ovo for the sustained release mechanism. The PLA-VEGF microparticles and the recombinant VEGF121 were explored for their bioactivity in human umbilical vein endothelial cells (HUVEC).

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Despite the potential of improving the delivery of epigenetic drugs, the subsequent assessment of changes in their epigenetic activity is largely dependent on the availability of a suitable and rapid screening bioassay. Here, we describe a cell-based assay system for screening gene reactivation.

11p vijisoo2711 29-09-2020 11 1   Download

Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the most popular strategies for genetic material transduction.

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Recent progress in development of the CRISPR/Cas9 system has been shown to be an efficient gene-editing technology in various organisms. We recently developed a novel method called Genome-editing via Oviductal Nucleic Acids Delivery (GONAD) in mice; a novel in vivo genome editing system that does not require ex vivo handling of embryos, and this technology is newly developed and renamed as “improved GONAD” (i-GONAD).

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The use of toxins for cancer therapy has great promise. Gelonin, a potent plant toxin, causes cell death by inactivating the 60S ribosomal subunit. Recently, we developed a novel gene delivery system using biodegradable cationic heparin-polyethyleneimine (HPEI) nanogels. In the current study, the antitumor activity of a recombinant plasmid expressing gelonin (pGelonin) on human ovarian cancer was assessed.

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Recently, carbon dots (CDs) have attracted great attention due to their superior properties, such as biocompatibility, fluorescence, high quantum yield, and uniform distribution. These characteristics make CDs interesting for bioimaging, therapeutic delivery, optogenetics, and theranostics. Photoluminescence (PL) properties enable CDs to act as imaging-trackable gene nanocarriers, while cationic CDs with high transfection efficiency have been applied for plasmid DNA and siRNA delivery.

13p caygaocaolon1 13-11-2019 7 0   Download

(BQ) Part 1 of the document Head and neck surgery in otorhinolaryngology 17 has contents: Anatomy of the auditory and vestibular systems, development of the ear, molecular biology of hearing and balance, physiology of the auditory and vestibular systems, inner ear drug delivery and gene therapy,…. And other contents. Invite you to refer.

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Nonviral gene delivery systems are considered to be a safe alternative to the viral system used in gene therapy. Although polyethylenimine (PEI) is among the most promising gene-carrier candidates for efficient nonviral gene delivery, safety concerns regarding its toxicity remain challenging.

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part 2 book “drug delivery - an integrated clinical and engineering approach” has contents: microsponges for drug delivery, chitosan for advancing drug delivery, gene delivery by electroporation, intrathecal drug delivery, cardiac drug delivery, drug delivery systems, intravitreal drug delivery,… and other contents.

338p tieu_vu14 15-08-2018 16 3   Download

Special oligonucleotides for targeted gene correction have attracted increasing attention recently, one of which is the chimericRNAÆDNAoligonucleotide (RDO) system. RDOs for targeted gene correction were first designed in 1996, and are typically 68 nucleotides in length including continuous RNA andDNA sequences (RNA is 2¢-O-methyl-modified).

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A bio-nanocapsule (BNC), composed of the surface antigen (sAg) of the hepatitis B virus, is an efficient nanomachine with which to accomplish the liver-specific delivery of genes and drugs. Approximately 110 molecules of sAg are associated to form a BNC particle with an average diameter of 130 nm. TheLprotein is an sAg peptide composed mainly of preS and S regions. The preS region, with specific affinity for human hepatocytes, is localized in the N-terminus.

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Polymeric micelle, the self-assembly of block copolymers with core-shell architecture, is a promising nanocarrier for drug and gene delivery. There are several relevant properties in polymeric micelle as nanocarrier systems, such as longevity in blood circula-tion, tissue-penetrating ability, spatial and temporal controlled drug release, and reduced inherent toxicity of cytotoxic reagents.

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This plan sets out our high level business priorities and delivery plans for 2012-2013, not only to provide core services to offenders in prison and on probation in England and Wales, but also to continue to improve the way we work, to achieve better outcomes for the public at less cost to the taxpayer. The reforms and activities in this plan are part of the wider Ministry of Justice Transforming Justice programme, to deliver a justice system which is more effective, less costly, and more responsive to the public.

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RNA interference (RNAi) has been regarded as a revolutionary tool for manipulating target biological processes as well as an emerging and prom-ising therapeutic strategy. In contrast to the tangible and obvious effective-ness of RNAiin vitro, silencing target gene expression in vivo using small interfering RNA (siRNA) has been a very challenging task due to multiscale barriers

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This contribution book collects reviews and original articles from eminent experts working in the interdisciplinary arena of novel drug delivery systems and their uses. From their direct and recent experience, the readers can achieve a wide vision on the new and ongoing potentialities of different drug delivery systems. Since the advent of analytical techniques and capabilities to measure particle sizes in nanometer ranges, there has been tremendous interest in the use of nanoparticles for more efficient methods of drug delivery.

512p camchuong_1 10-12-2012 49 2   Download