Gene identification

Bacterial infections have a substantial impact on global health and can become serious if misdiagnosed with several diseases related to the central nervous, cardiovascular, and respiratory systems. The prognosis in patients with infectious disease strongly depends on early diagnosis and appropriate antibiotic therapy. We aimed to compare the accuracy of genus and species-level identification bacteria using biochemical testing and 16S rRNA sequence analysis.

8p viharuno 03-01-2025 1 1   Download

Phosphoinositide-specific phospholipase C (PI-PLC) has been known as one of the key enzymes that involved in the phospholipid hydrolysis. However, the PI-PLC family in cassava has not been fully recorded. In this study, a comprehensive analysis of the PI-PLC family in cassava assembly has been performed based on various bioinformatics tools.

9p viprimi 16-12-2024 2 1   Download

Research objective: Establish the genetic variants database of 04 genes CYP2C9, CYP2C19, CYP2D6 and CYP3A5 in Kinh Vietnamese. Identification of genotype and allele frequencies of 04 genes CYP2C9, CYP2C19, CYP2D6 and CYP3A5 in Kinh Vietnamese. Perform in silico functional analysis of novel variants.

27p extraenglish 24-05-2021 27 4   Download

Establish the genetic variants database of 04 genes CYP2C9, CYP2C19, CYP2D6 and CYP3A5 in Kinh Vietnamese. Identification of genotype and allele frequencies of 04 genes CYP2C9, CYP2C19, CYP2D6 and CYP3A5 in Kinh Vietnamese. Perform in silico functional analysis of novel variants.

27p capheviahe27 23-02-2021 5 3   Download

Evaluate genetic diversity of Lien Minh chickens based on sequences of mitochondrial DNA D-loop region; determine the genetic relationship and the origin of Lien Minh chickens; identif genetic relationships between candidate genes and egg yield traits in Lien Minh chickens.

27p kequaidan7 01-09-2020 33 3   Download

Understanding the genetic complexity of traits is an important objective of small grain temperate cereals yield and adaptation improvements. Bi-parental quantitative trait loci (QTL) linkage mapping is a powerful method to identify genetic regions that co-segregate in the trait of interest within the research population. However, recently, association or linkage disequilibrium (LD) mapping using a genome-wide association study (GWAS) became an approach for unraveling the molecular genetic basis underlying the natural phenotypic variation.

17p partimesinhvien 08-05-2020 17 1   Download

We have identified and characterized a thermostable thio-redoxin system in the aerobic hyperthermophilic archaeon AeropyrumpernixK1. Thegene (Accessionno.APE0641) of A. pernixencoding a 37 kDaprotein contains a redox active site motif (CPHC) but its N-terminal extension region (about 200 residues) shows no homologywithin the genome database. A second gene (Accession no. APE1061) has high homology to thioredoxin reductase and encodes a 37 kDa protein with the active site motif (CSVC), and binding sites forFADandNADPH....

8p tumor12 22-04-2013 34 2   Download

Antimicrobial peptides form one of the first lines of defense against invading pathogens by killing the microorganisms and/ormobilizing the host innate immune system. Although over 800 antimicrobial peptides have been isolated from many different species, especially insects, few have been reported frommarinefish. Sequenceanalysisof twogenomic clones (15.6 and 12.5 kb) from the winter flounder, Pseudopleuronectes americanus(Walbaum) resulted in the identification of multiple clustered genes for novel pleuro-cidin-like antimicrobial peptides....

11p tumor12 20-04-2013 28 2   Download

Glucose transporters (GLUTs) have been implicated in adaptive and survival responses to hypoxic stress in mam-mals. In fish, the expression and regulation ofGLUT in relation to hypoxia remains unexplored. Here we describe the identification ofa hypoxia-responsive glucose transpor-ter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans
11 kb ofgenomic sequence and consists of12 exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a poly-peptide of533 amino acids, with a predictedmolecularmass of
57 kDa and a pI of8.34....

8p fptmusic 16-04-2013 41 3   Download

The pyruvate dehydrogenase complex occupies a central and strategic posi-tion in muscle intermediary metabolism and is primarily regulated by phos-phorylation⁄dephosphorylation. The identification of multiple isoforms of pyruvate dehydrogenase kinase (PDK1–4) and pyruvate dehydrogenase phosphatase (PDP1–2) has raised intriguing new possibilities for chronic pyruvate dehydrogenase complex control.

11p awards 06-04-2013 38 2   Download

accharomyces cerevisiaeis a widely used host in the pro-ductionof therapeutic peptides andproteins.Herewe report the identification of a novel endoprotease in S. cerevisiae. It is encoded by the CYM1gene and is specific for the C-terminus of basic residues of heterologously expressed peptides. Gene disruption ofCYM1not only reduced the intracellular proteolysis, but also enhanced the secretion of heterologously expressed peptides such as growth hormone, pro-B-type natriuretic peptide and pro-cholecystokinin....

10p awards 05-04-2013 39 3   Download

Conservation of microRNAs (miRNAs) among species suggests that they bear conserved biological functions. However, sequencing of new miRNAs has not always been accompanied by a search for orthologues in other spe-cies. I report herein the results of a systematic search for interspecies ortho-logues of miRNA precursors, leading to the identification of 35 human and 45 mouse new putative miRNA genes. MicroRNA tracks were written to visualize miRNAs in human and mouse genomes on the UCSC Genome Browser....

15p awards 05-04-2013 39 7   Download

Anserinase (Xaa-methyl-His dipeptidase, EC 3.4.13.5) is a dipeptidase that mainly catalyzes the hydrolysis ofNa-acetylhistidine in the brain, retina and vitreous body of all poikilothermic vertebrates. The gene encoding anserinase has not been previously identified. We report the molecular identification of anserinase, purified from brain of Nile tilapia Oreochromis niloticus.

13p dell39 27-03-2013 42 5   Download

Cell-free protein synthesis using cell extracts from Escherichia coli, wheat germ and rabbit reticulocytes has been used for over 40 years to produce small amounts of radiolabeled proteins for identification of gene products and other applications. In the E. coli system programmed with plasmid DNA, the cell extract contains or is supplemented with an RNA po-lymerase to transcribe the gene, and the mRNAs are translated by a complex mixture that contains ribo-somes and a full complement of initiation,...

2p inspiron33 25-03-2013 44 6   Download

The identification of TUBBY-like genes in organisms ranging from single-celled to multicellular eukaryotes has allowed the phylogenetic history of this gene family to be traced back to the early evolutionary stages of eukaryote development.

9p media19 05-03-2013 29 1   Download

Fish has been recently recognized as a suitable vertebrate model and repre-sents a promising alternative to mammals for studying mechanisms of tis-sue mineralization and unravelling specific questions related to vertebrate bone formation. The recently developedSparus aurata(gilthead seabream) osteoblast-like cell line VSa16 was used to construct a cDNA subtractive library aimed at the identification of genes associated with fish tissue min-eralization.

12p media19 04-03-2013 32 2   Download

In living organisms, genes encoding proteins that contain flavins as a pros-thetic group constitute approximately 2–3% of the total. The fluorescence of flavin cofactors in these proteins is a property that is widely employed for biochemical characterisation. Here, we present a modified Thermofluor  approach called ThermoFAD (Thermofluor  -adapted flavin ad hoc detec-tion system), which simplifies identification of optimal purification and storage conditions as well as high-affinity ligands.

8p viettel02 22-02-2013 39 5   Download

The isovaleric acid-emanating silkworm mutantskunk (sku) was first stud-ied over 30 years ago because of its unusual odour and prepupal lethality. Here, we report the identification and characterization of the gene responsi-ble for theskumutant.

12p viettel02 19-02-2013 33 2   Download

In the present study, we report the identification of a new gene from the Bacillus subtilisB3 strain (aatB3), which comprises 1308 bp encoding a 436 amino acid protein with a monomer molecular weight of 49.1 kDa. Phylo-genetic analyses suggested that this enzyme is a member of the Ib subgroup of aspartate aminotransferases

13p cosis54 05-01-2013 52 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptomic identification of candidate genes involved in sunflower responses to chilling and salt stresses based on cDNA microarray analysis

18p panasonic06 26-12-2011 54 4   Download