Gene sequences

The aim of this study was to investigate the duplication events within the SABATH gene family in cassava using computational tools. By analyzing the similarities between coding DNA sequences, we identified potential duplicated gene pairs and examined their structures. In addition, we assessed the expression profiles of SABATH genes across various cassava tissues during key growth and developmental stages.

10p viaburame 14-03-2025 6 1   Download

Hypertrophic cardiomyopathy (HCM) is a common genetic cardiovascular disease and a major cause of sudden death. It is also involved in increasing morbidity and mortality of various cardiovascular diseases. Genetic factors have been found to be important in determining the phenotypic manifestation of cardiac hypertrophy. However, only 50–60% of HCM patients have been identified as having pathogenic variants in known genes, suggesting that more studies are needed to find more disease genes.

15p xuanphongdacy06 18-09-2024 8 1   Download

This study was conducted to determine the composition of Toxocara species in dogs and cats in Vietnam. One hundred and twenty-seven Toxocara adult worms were collected from dogs and cats and were analyzed by PCR-RFLP assay and DNA sequencing of a partial section of the cox1 gene.

7p vibecca 01-10-2024 4 2   Download

This study aimed to develop a simple sequencing protocol by the Sanger method using fewer primers and reactions for genotyping the HLA-C gene than published protocols. The simple protocol with three primers includes one PCR reaction and two sequencing reactions.

8p vihyuga 04-03-2025 4 1   Download

Vibrio parahaemolyticus is one of the dangerous pathogens of aquatic animals, including fish. The outer membrane proteins play an essential role in bacterial virulence and are potential candidates for vaccine development. In this study, the virulence of four V. parahaemolyticus strains was studied, and the result was that strain. The V. parahaemolyticus N9 has the strongest virulence with an LD50 of 106.15 CFU/mL.

8p viinuzuka 28-02-2025 5 1   Download

Mục tiêu của nghiên cứu này là đánh giá sự đa dạng di truyền, mối quan hệ di truyền của các mẫu giống xoài được thu thập tại khu vực núi Bà Đen thuộc tỉnh Tây Ninh dựa trên chỉ thị hình sinh học phân tử SSR (Simple Sequence Repeats), nhằm phục vụ cho công tác bảo tồn nguồn gene.

11p tuetuebinhan000 23-01-2025 6 3   Download

Bacterial infections have a substantial impact on global health and can become serious if misdiagnosed with several diseases related to the central nervous, cardiovascular, and respiratory systems. The prognosis in patients with infectious disease strongly depends on early diagnosis and appropriate antibiotic therapy. We aimed to compare the accuracy of genus and species-level identification bacteria using biochemical testing and 16S rRNA sequence analysis.

8p viharuno 03-01-2025 4 2   Download

Cấu trúc của gene 1. Các intron và exon Cấu trúc intron và exon của gene là một đặc điểm để phân biệt giữa ADN của sinh vật eukaryote và prokaryote. Các intron chiếm phần lớn trong cấu trúc hầu hết các gene của cơ thể eukaryote. Các đoạn intron này sẽ được các enzyme cắt một cách chính xác ra khỏi các phân tử mRNA trước khi chúng đi ra khỏi nhân tế bào. Vị trí cắt của các enzyme được xác định bởi các đoạn ADN được gọi là các đoạn đồng nhất (consensus sequences) (đoạn này có tên như...

4p butmauvang 29-08-2013 189 6   Download

Bài giảng Công nghệ Gene: Chương 3 Giải trình tự Gene, bộ Gene, cung cấp cho người học những kiến thức như: Giải trình tự gene và bộ gene bằng phương pháp Sanger (chain-termination DNA sequencing); Giải trình tự gene và bộ gene bằng phương pháp mới (NGS-next generation sequencing): Illumina; Ứng dụng của kỹ thuật giải trình tự DNA. Mời các bạn cùng tham khảo!

15p caphesuadathemhanh 03-12-2021 26 3   Download

Analysis of the rate of resistance to Amoxicillin, Clarithromycin and efficacy of Esomeprazole-Amoxicilin-Clarithromycin (EAC) regimen in Helicobacter pylori positive duodenal ulcer. Determinate the recrudescence and reinfection status of Helicobacter pylori after successful eradication treatment, by PCR-RFLP technique and UreC gene sequencing.

27p kequaidan12 03-06-2021 18 4   Download

Research purposes of the dissertation: Studying on Dalbergia tonkinensis Prain systematicly and fully about: plants (microsurgery, gene sequences), chemical compositions and biological activity.

27p capheviahe27 23-02-2021 18 3   Download

Evaluate genetic diversity of Lien Minh chickens based on sequences of mitochondrial DNA D-loop region; determine the genetic relationship and the origin of Lien Minh chickens; identif genetic relationships between candidate genes and egg yield traits in Lien Minh chickens.

27p kequaidan7 01-09-2020 33 3   Download

MicroRNAs (miRNAs) are small noncoding RNAs of about 19–25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known.

10p nutifooddau 27-01-2019 35 3   Download

Objectives of thesic: Isolation, point mutation of P5CS gene conferring drought tolerance and transformion into soybean varieties of Vietnam: Comparing the DNA sequence of P5CS isolated from different soybean varieties and producing mutant P5CS without feedback inhibition by proline.

21p nguyenthiminh32 12-07-2014 91 5   Download

We report here the direct evidence for the formation of alternative DNA structures in a plasmid DNA, termed pTIR10, containing a 0.23-kb pyrimidine/purine-biased (Pyr/Pur) stretch isolated from the rat genome. Long Pyr/Pur sequences are abundant in eukaryotic genomes, and they may modulate the biological activity of genes and genomes via formation of various types of triplex-related structures. The plasmid DNA in sodium acetate buffer (pH 4.35) was deposited on APS-modified mica, and after drying it was imaged with an atomic force microscope in air. ...

5p system191 01-06-2013 34 3   Download

Pyruvate decarboxylase (EC 4.1.1.1) was isolated and purified from the yeast Kluyveromyces lactis. The properties of this enzyme relating to the native oligomeric state, the subunit size, the nucleotide sequence of the coding gene(s), the catalytic activity, and protein fluorescence as well as circular dichroism are very similar to those of the well characterized pyruvate decarboxylase species from yeast.

8p system191 01-06-2013 45 3   Download

We report here the full coding sequence of a novel mouse putative membrane-associated mucin containing three extracellular EGF-like motifs and a mucin-like domain consisting of at least 20 tandem repeats of 124–126 amino acids. Screening a cosmid and a BAC libraries allowed to isolate several genomic clones. Genomic and cDNA sequence comparisons showed that the gene consists of 25 exons and 24 introns covering a genomic region of  52 kb. The first intron is  16 kb in length and is followed by an unusually large exon ( 9.5 kb) encoding Ser/Thr-rich tandemly repeated sequences....

10p system191 01-06-2013 49 4   Download

SHP-1, a protein-tyrosine phosphatase with two srchomology 2 domains, is expressed predominantly in hematopoietic and epithelial cells and has been implicated in numerous signaling pathways as a negative regulator. Two promoters direct the expression of human and murine SHP-1, and two types of transcripts (I) and (II) SHP-1, are initiated from each of these promoters. The cDNA sequences of (I)SHP-1 and (II)SHP-1 are identical except in the 5¢ untranslated region and in the first few coding nucleotides.

8p system191 01-06-2013 41 3   Download

Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of a genomic library of G. graminis yielded a full-length clone of the Mn-Lipoxygenase gene. cDNA analysis showed that the gene spanned 2.6 kb and contained one intron (133 bp). Northern blot analyses indicated two transcripts (2.7 and 3.1 kb). The deduced amino-acid sequence of the Mn-Lipoxygenase precursor (618 amino acids, 67.

8p system191 01-06-2013 41 4   Download

Two ORFs, cphA and cphB, encoding proteins CphA and CphB with strong similarities to plant phytochromes and to the cyanobacterial phytochrome Cph1 of Synechocystis sp. PCC 6803 have been identified in the filamentous cyanobacterium Calothrix sp. PCC7601. While CphA carries a cysteine within a highly conserved amino-acid sequence motif, to which the chromophore phytochromobilin is covalently bound in plant phytochromes, in CphB this position is changed into a leucine.

10p system191 01-06-2013 45 4   Download