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Genetic perturbations
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A complex set of perturbations occur in cytokines and hormones in the etiopathogenesis of obesity and related cardiometabolic conditions such as type 2 diabetes (T2D). Evidence for the genetic regulation of these cytokines and hormones is limited, particularly in African-ancestry populations. In order to improve our understanding of the biology of cardiometabolic traits.
21p
vibransone
28-03-2024
2
2
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Identification of causal genes for polygenic human diseases has been extremely challenging, and our understanding of how physiological and pharmacological stimuli modulate genetic risk at disease-associated loci is limited.
16p
viellison
28-03-2024
7
2
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Genetic screens have been the lifeblood of forward genetics. They have enabled widespread discoveries of gene function, leading to meaningful advances in medicine, biotechnology, and agriculture. Nevertheless, technologies for performing these screens have been limited by scale, specificity, and targeting range of tools for investigating and perturbing the genome.
4p
vimichaelfaraday
25-03-2022
11
1
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Systems for CRISPR-based combinatorial perturbation of two or more genes are emerging as powerful tools for uncovering genetic interactions. However, systematic identification of these relationships is complicated by sample, reagent, and biological variability.
10p
vigalileogalilei
27-02-2022
7
1
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Pooled CRISPR screens are a powerful tool to probe genotype-phenotype relationships at genome-wide scale. However, criteria for optimal design are missing, and it remains unclear how experimental parameters affect results. Here, we report that random decreases in gRNA abundance are more likely than increases due to bottle-neck effects during the cell proliferation phase. Failure to consider this asymmetry leads to loss of detection power.
13p
viarchimedes
26-01-2022
5
0
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An important unanswered question in chromatin biology is the extent to which long-range looping interactions change across developmental models, genetic perturbations, drug treatments, and disease states. Computational tools for rigorous assessment of cell type-specific loops across multiple biological conditions are needed.
44p
viarchimedes
26-01-2022
10
1
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Understanding the consequences of thermal and chemical variations in aquatic habitats is of importance in a scenario of global change. In ecology, the sex ratio is a major population demographic parameter. So far, research that measured environmental perturbations on fish sex ratios has usually involved a few model species with a strong genetic basis of sex determination, and focused on the study of juvenile or adult gonads. However, the underlying mechanisms at the time of gender commitment are poorly understood.
13p
vilarryellison
29-10-2021
9
1
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Large-scale genetic screening using CRISPR-Cas9 technology has emerged as a powerful approach to uncover and validate gene functions. The ability to control the timing of genetic perturbation during CRISPR screens will facilitate precise dissection of dynamic and complex biological processes.
15p
visilicon2711
20-08-2021
4
1
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Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function.
15p
vijeeni2711
24-07-2021
1
0
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In bacteria, pan-genomes are the result of an evolutionary “tug of war” between selection and horizontal gene transfer (HGT). High rates of HGT increase the genetic pool and the effective population size (Ne), resulting in open pan-genomes.
18p
vijeeni2711
24-07-2021
11
0
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Gene perturbation experiments in combination with fluorescence time-lapse cell imaging are a powerful tool in reverse genetics. High content applications require tools for the automated processing of the large amounts of data.
10p
viwyoming2711
16-12-2020
8
1
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The combination of time-lapse imaging of live cells with high-throughput perturbation assays is a powerful tool for genetics and cell biology. The Mitocheck project employed this technique to associate thousands of genes with transient biological phenotypes in cell division, cell death and migration.
10p
viwyoming2711
16-12-2020
8
1
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Transcription factor (TF) networks play a key role in controlling the transfer of genetic information from gene to mRNA. Much progress has been made on understanding and reverse-engineering TF network topologies using a range of experimental and theoretical methodologies.
13p
viflorida2711
30-10-2020
18
2
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Gene regulatory networks (GRNs) can be inferred from both gene expression data and genetic perturbations. Under different conditions, the gene data of the same gene set may be different from each other, which results in different GRNs.
13p
vicolorado2711
23-10-2020
14
1
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Ewing sarcoma is a malignancy of primitive cells, possibly of mesenchymal origin. It is probable that genetic perturbations other than EWS-FLI1 cooperate with it to produce the tumor. Sequencing studies identified STAG2 mutations in approximately 15% of cases in humans.
14p
vikuala271
13-06-2020
4
0
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Molecular characterization is an essential step of risk/safety assessment of genetically modified (GM) crops. Holistic approaches for molecular characterization using omics platforms can be used to confirm the intended impact of the genetic engineering, but can also reveal the unintended changes at the omics level as a first assessment of potential risks.
12p
viminato2711
22-05-2020
7
1
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Osmotic homeostasis is a fundamental requirement for life. In general, the effector mechanisms that mediate cellular and extracellular osmoregulation in animals are reasonably well defined. However, at the molecular level, little is known about how animals detect osmotic and ionic perturbations and transduce them into regulatory responses.
8p
media19
04-03-2013
29
1
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Proteins hosting mainb-sheets adopt specific strategies to avoid intermolec-ular interactions leading to aggregation and amyloid deposition. Human beta-2 microglobulin (b2m) displays a typical immunoglobulin fold and is known to be amyloidogenicin vivo.
10p
cosis54
05-01-2013
32
3
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Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA. †Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA. ‡Current address: DOE Joint Genome Institute, 2800 Mitchell Drive, Bldg 400, Walnut Creek, CA 94596, USA. Correspondence: Stephanie W Ruby. E-mail: sruby@unm.edu reviews Published: 30 March 2004 Genome Biology 2004, 5:R29 The electronic version of this article is the complete one and can be found online at http://genomebiology.
0p
thulanh21
15-11-2011
47
3
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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Prioritizing functional modules mediating genetic perturbations and their phenotypic effects: a global strategy...
0p
thulanh20
12-11-2011
49
1
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