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Genotyping technique
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The incidence and prevalence of nontuberculous mycobacterial disease is increasing due to enhanced clinician awareness and improved detection methods. The species identification using molecular microbiology techniques allows a better understanding of the differences in pathogenicity and treatment response.
2p
viintuit
26-09-2023
0
0
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Genotyping-by-sequencing (GBS) has emerged as a powerful and cost-effective approach for discovering and genotyping single-nucleotide polymorphisms. The GBS technique was largely used in crop species where its low sequence coverage is not a drawback for calling genotypes because inbred lines are almost homozygous.
14p
vinarcissa
21-03-2023
3
1
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Genotype imputation is a common technique in genetic research. Genetic similarity between target population and reference dataset is crucial for high-quality results. Although several reference panels are available, it is often not clear which is the most optimal for a particular target dataset to be imputed.
16p
vinarcissa
21-03-2023
4
1
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Polyploidization is an important technique used in grape breeding to create new genetic resources and can be induced using some antimitotic agents, including colchicine. Plants with increased ploidy can gain better quality than their original diploids in some characteristics. "Induction of polyploidy in grapevine (Vitis vinifera L.) seedlings by in vivo colchicine applications" was carried out to obtain polyploid genotypes under in vivo conditions.
8p
lyhuyenthu
31-01-2023
3
2
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The correlation between various factors (geographical region, clinical incidence, and host type) and the genomic heterogeneity has been shown in several bacterial strains including Chlamydia abortus.
12p
vidarwin
22-02-2022
10
1
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Anther culture response of six wheat genotypes and their F1 crosses was investigated. Results indicated that considerable genetic variation among the tested genotypes was observed. Calli were obtained from all wheat genotypes studied.
10p
schindler
30-12-2021
5
0
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The SDS–PAGE and ISSRPCR techniques were used to detect some biochemical and molecular markers associated with drought tolerance in six local and exotic genotypes of lentil (Sinai 1, Giza 9, L 9, L 16, L 20 and L 25) under rainfed conditions at El-sheikh Zowaid, North Sinai.
8p
schindler
30-12-2021
7
0
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Genotypes not directly measured in genetic studies are often imputed to improve statistical power and to increase mapping resolution. The accuracy of standard imputation techniques strongly depends on the similarity of linkage disequilibrium (LD) patterns in the study and reference populations. Here we develop a novel approach for genotype imputation in low-recombination regions that relies on the coalescent and permits to explicitly account for population demographic factors.
12p
vilarryellison
29-10-2021
10
1
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Bread wheat has a large complex genome that makes whole genome resequencing costly. Therefore, genome complexity reduction techniques such as sequence capture make re-sequencing cost effective. With a highquality draft wheat genome now available it is possible to design capture probe sets and to use them to accurately genotype and anchor SNPs to the genome.
12p
vibeauty
23-10-2021
15
2
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Understanding the underlying genetic structure of human populations is of fundamental interest to both biological and social sciences. Advances in high-throughput genotyping technology have markedly improved our understanding of global patterns of human genetic variation. The most widely used methods for collecting variant information at the DNA-level include whole genome sequencing, which remains costly, and the more economical solution of array-based techniques, as these are capable of simultaneously genotyping a pre-selected set of variable DNA sites in the human genome.
13p
vitzuyu2711
29-09-2021
14
1
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Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR.
10p
vitzuyu2711
29-09-2021
11
1
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Host genetic backgrounds affect gene functions. The genetic backgrounds of genetically engineered organisms must be identified to confirm their genetic backgrounds identity with those of recipients. Marker-assisted backcrossing (MAB), transgenesis and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (CRISPR/Cas9) editing are three commonly used genetic engineering techniques.
12p
visilicon2711
20-08-2021
6
1
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Mixed infections of Mycobacterium tuberculosis and antibiotic heteroresistance continue to complicate tuberculosis (TB) diagnosis and treatment. Detection of mixed infections has been limited to molecular genotyping techniques, which lack the sensitivity and resolution to accurately estimate the multiplicity of TB infections.
16p
vijeeni2711
24-07-2021
9
0
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Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Production of pure lines by DH induction provides a new way to achieve homozygosity earlier in B.napus.
13p
vijichea2711
28-05-2021
19
1
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Chickpea is best nutritional crop in leguminous species, and biotic and abiotic stress affects the yield loss around 50 to 60 % .For preventing yield loss from this stress, it is necessary to developed high yield, qualitative variety of chickpea. In vitro methods are best technique for regeneration and development of transgenic variety of chickpea. In this investigation research to determine chickpea in vitro micropropagation was studied in genotypes; of chickpea JG62, JG63 JG315, VIJAY and Bhupdachana with explants immature cotyledonary and embryonic axis.
10p
cothumenhmong11
11-05-2021
5
1
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Analysis and comparison of the transcriptome of human hepatocytes infected with the Yellow Fever virus to verify the transcriptional profile of both genotypes, in response to viral infection. The sample used corresponds to strains of YFV code H622205, from the state of Goiás-GO (Brazil), a fatal human case isolated from the patient's blood and serum.
14p
trinhthamhodang11
27-04-2021
17
2
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The information in Indian goats in particular Surti, is less. So the present study was planned with the objective to study the polymorphism in growth hormone gene using PCR-RFLP technique and to explore the association of various genotypes of the gene with the growth related parameters in Surti goats.
6p
trinhthamhodang11
27-04-2021
8
1
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This study was undertaken to assess the selection criteria for identifying drought tolerance in cotton varieties, so that suitable varieties can be recommended for cultivation in drought prone areas.
10p
caygaocaolon11
21-04-2021
13
1
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=Sugarcane (Saccharum spp.) is an important commercial crop, cultivated across the world. Smut disease is one of the major diseases of sugarcane. Severe smut infection affects the sugar recovery as well as yield loss ranging from 10 to70 %. The present study was based on the evaluation of seventy five sugarcane genotypes against smut disease by following seed sett inoculation technique under field condition to screen out the newer sources of resistance as well as susceptible for the development of the smut resistant varieties.
11p
chauchaungayxua10
19-03-2021
7
2
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An experiment was conducted to evaluate salinity tolerance of promising sugarcane genotypes with microplot techniques at Agricultural Research Station, Perumallapalle, ANGRAU, Andhra Pradesh during 2018-19.
6p
chauchaungayxua10
18-03-2021
8
1
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