Guanine nucleotide exchange factors
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Small GTP binding protein Rac1 is a component of NADPH oxidases and is essential for superoxide-induced cell death. Rac1 is activated by guanine nucleotide exchange factors (GEFs), and this activation can be blocked by regulator of chromosome condensation 2 (RCC2), which binds the switch regions of Rac1 to prevent access from GEFs.
11p vibaku2711 22-07-2020 14 1 Download
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Plants perceive UV-B through the UV RESISTANCE LOCUS 8 (UVR8) photoreceptor and UVR8 activation leads to changes in gene expression such as those associated with UV-B acclimation and stress tolerance.
11p vihashirama2711 21-05-2020 14 1 Download
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Erythroid dematin is a major component of red blood cell junctional complexes that link the spectrin±actin cytoskel-eton to the overlying plasma membrane. Transcripts of dematinarewidelydistributed includinghumanbrain, heart, lung, skeletalmuscle, andkidney.Invitro, dematinbinds and bundles actin ®laments in a phosphorylation-dependent manner. The primary structure of dematin consists of a C-terminal domain homologous to theÔheadpieceÕ domain of villin, an actin-binding protein of the brush border cyto-skeleton. ...
12p research12 23-04-2013 40 1 Download
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Elongation factor Ts (EF-Ts) is the guanine nucleotide-exchange factor for elongation factor Tu (EF-Tu) that is responsible for promoting the binding of aminoacyl-tRNA to the mRNA-programmed ribosome.The struc-ture of the Escherichia coliEF-Tu–EF-Ts complex reveals a protruding antiparallel coiled-coil motif in EF-Ts, which is responsible for the dimerization of EF-Ts in the crystal. In this study, the sequence encoding the coiled-coil motif in EF-Ts was deleted from the genome in Escherichia coli by gene replacement....
12p tumor12 20-04-2013 24 2 Download
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We have previously reported the Ras-dependent activation of the mitogen-activated protein kinases p44 and p42, also termed extracellular signal-regulated kinases (ERK)1 and 2 (ERK1⁄2), mediated through Gs-coupled serotonin receptors transiently expressed in human embryonic kidney (HEK) 293 cells. Whereas G i- and Gq-coupled receptors have been shown to activate Ras through the guanine nucleotide exchange factor (GEF) called Ras-GRF1 (CDC25 Mm ) by binding of Ca 2+ ⁄calmodulin to its N-terminal IQ domain, ...
0p awards 06-04-2013 32 1 Download
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RasGRF is a family of guanine nucleotide exchange factors with dual spe-cificity for both Ras and Rac GTPases. In this study, using mouse brain extracts, we show that both RasGRF1 and RasGRF2 interact with micro-tubules in an in vitro microtubule assembly system and this binding is very tight. To characterize this association, recombinant purified proteins con-taining different regions of RasGRF1 were tested for their ability to bind microtubules preassembled from pure tubulin.
12p inspiron33 26-03-2013 47 4 Download
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The voltage-gated potassium channel Kv4.3 was coexpressed with itsb-sub-unit Kv channel-interacting protein 2 and the angiotensin type 1 receptor in HEK-293 cells. Proteomic analysis of proteins coimmunoprecipitated with Kv4.3 revealed that Kv4.3 is associated with Rap guanine nucleotide exchange factors MR-GEF and EPAC-1.
10p media19 04-03-2013 34 1 Download
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The highly conserved RasGEF1 family of proteins contain a C-terminal CDC25-Ras exchange motif domain and an N-terminal RasGEF-N domain, and are of unknown function and specificity. Using purified Ras-GEF1A and RasGEF1B proteins, as well as Ras family proteins, we estab-lished that RasGEF1A and RasGEF1B function as very specific exchange factors for Rap2, a member of the Rap subfamily of Ras-like G-proteins.
10p vinaphone15 25-02-2013 32 1 Download
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The kinase noncatalytic C-lobe domain (KIND) is a putative protein–protein interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclear factor), PTPN13 (protein tyrosine phosphatase), FRMPD2 (scaffold protein) and very-KIND (v-KIND) (brain-specific Ras guanine nucleotide exchange factor), have been identified to date.
11p cosis54 05-01-2013 35 2 Download
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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue...
13p thulanh15 18-10-2011 57 1 Download
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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs...
19p toshiba3 07-09-2011 52 4 Download