High thermal stability

Pwo DNA polymerase is an enzyme that is widely used in PCR due to its thermal stability and proofreading activity (3′-5′ exonuclease activity) with high accuracy. Optimizing the expression and purification of the protein is crucial to reducing the cost of production for this important enzyme. In this study, the higher expression level of the gene encoding Pwo DNA polymerase in bacteria strain E. coli BL21(DE3) was achieved by optimizing codon usage.

11p xuanphongdacy06 18-09-2024 2 1   Download

The aim of the work "Activated carbon derived from rice husk by NaOH activation and its application in supercapacitor" is to prepare a low-cost, high specific surface area activated carbon with microporous and mesoporous ranges using rice husk as the raw material. The effects of activation temperature on the specific surface area, pore structure, morphology and thermal stability of the AC samples have been examined. The AC samples were proved to be excellent electrode material for supercapacitor, hence solve the disposal and pollution problems of rice husk.

9p runordie3 27-06-2022 12 3   Download

The catalyst 7.5% NiO/TiO2 and seven samples of NiO/γ-Al2O3 with NiO content from 7.5% to 60% have been obtained and studied. Physico-chemical characteristics of the catalysts were determined by methods of Adsorption (BET), X-ray Diffraction (XRD), TemperatureProgrammed Reduction (TPR) and Hydrogen Pulse Titration. The catalytic properties of the obtained samples were investigated in the reaction of CO methanation at temperatures 180 – 220◦C and mole ratios hydrogen/carbon monoxide 25-100.

16p 12120609 01-06-2020 6 1   Download

At the present, in order to increase octan number the izomerization of hydrocarbon has been studied in the world. Amongs metals and metal oxides on carriers, Pt on the alumina carrier is used most widely owing to the advantages such as thermal stability, high activity and high selectivity etc. The conversion and selectivity of the izomerization depend on the catalysts (the composition of the catalysts, the structure of the carriers, the distribution of metal, the acidity, the catalytic activity of the catalysts) and the reaction condition (temperature, volume flow, carrier gases ...).

4p uocvong04 24-09-2015 62 4   Download

Oxidation of benzyl alcohol plays an important role in organic synthesis for the purpose of producing benzandehyde - a valuable chemical intermediates. MCM-41 is an important member of M41S mesoporous materials family. It is proved to be very potential for being used as support materials due to its high specific surface area, thermal-hydrothermal stability, etc.

5p uocvong03 24-09-2015 95 6   Download

The thermal stabilityofperoxidase fromleavesof theAfrican oil palm treeElaeis guineensis(AOPTP) at pH 3.0 was studied by differential scanning calorimetry (DSC), intrinsic fluorescence, CD and enzymatic assays.The spectral parameters asmonitoredby ellipticity changes in the far-UV CD spectrum of the enzyme as well as the increase in tryp-tophan intensity emission upon heating, together with changes in enzymatic activity with temperature were seen to be good complements to the highly sensitive but integral methodofDSC....

7p research12 29-04-2013 45 3   Download

The proposed biological function ofb-lactoglobulins as transporting proteins assumes a binding ability for ligands andhighstabilityunder theacidic conditionsof the stomach. This work shows that the conformational stability of non-ruminant porcine b-lactoglobulin (BLG) is not consistent with this hypothesis. Thermal denaturation of porcine BLG was studied by high-sensitivity differential scanning calori-metry within the pH range 2.0–10.0.

11p research12 23-04-2013 43 5   Download

The crystal structures of thermophilic xylanases from Chaetomium thermophilumandNonomuraea flexuosawere determined at 1.75 and 2.1 A˚ resolution, respectively. Both enzymes have the overall fold typical to family 11 xylanases with two highly twistedb-sheets forminga large cleft. The comparison of 12 crystal structures of family 11 xylanases from both mesophilic and thermophilic organisms showed that the structures of different xylanases arevery similar.

14p tumor12 20-04-2013 35 3   Download

To probe the stability of the seven-iron ferredoxin from Thermus thermophilus(FdTt), we investigated its chemical and thermal denaturationprocesses insolution.Aspredicted from the crystal structure, FdTt is extremely resistant to perturbation. The guanidine hydrochloride-induced unfolding transition shows a midpoint at 6.5M(pH 7, 20
C), andthe thermalmidpoint is aboveboiling, at 114
C. The stability of FdTt is much lower at acidic pH, suggesting that electrostatic interactions are important for the high stability at higher pH.

8p fptmusic 12-04-2013 58 5   Download

Neuroglobin (Ngb) and cytoglobin (Cygb), recent additions to the globin family, display a hexa-coordinated (bis-histidyl) heme in the absence of external ligands. Although these proteins have the classical globin fold they reveal a very high thermal stability with a melting temperature (Tm)of 100
C for Ngb and 95
C for Cygb. Moreover, flash photolysis experi-ments at high temperatures reveal that Ngb remains functional at 90
C

9p awards 06-04-2013 32 3   Download

We have studied the stability of the histone-like, DNA-binding protein HU from the hyperthermophilic eubacteriumThermotoga maritimaand its E34D mutant by differential scanningmicrocalorimetry and CDunder acidic conditions at various concentrations within the range of 2–225lMof monomer. The thermal unfolding of both proteins is highly reversible and clearly follows a two-state dissociation/unfolding model from the folded, dimeric state to the unfolded, monomericone.

11p dell39 03-04-2013 47 4   Download

Life grows almost everywhere on earth, including in extreme environments and under harsh conditions. Organisms adapted to high temperatures are called thermophiles (growth temperature 45–75C) and hyperthermophiles (growth temperature ‡80C). Proteins from such organisms usually show extreme thermal stability, despite having folded structures very similar to their mesostable counterparts.

11p media19 04-03-2013 52 3   Download

The hypothesis is tested that Jun–Fos activator protein-1 coiled coil inter-actions are dominated during late folding events by the formation of intri-cate intermolecular electrostatic contacts. A previously derived cJun–FosW was used as a template as it is a highly stable relative of the wild-type cJun–cFos coiled coil protein (thermal melting temperature = 63C versus 16C), allowing kinetic folding data to be readily extracted.

14p vinaphone15 27-02-2013 42 4   Download

Post-translational glycosylation is one of the most abundant forms of cova-lent protein modification in eukaryotic cells. It plays an important role in determining the properties of proteins, and stabilizes many proteins against thermal denaturation. Protein glycosylation may establish a surface micro-environment that resembles that of unglycosylated proteins in concentrated solutions of sugars and other polyols.

9p mobifone23 21-01-2013 43 4   Download