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Immature embryos
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Luận văn "Nghiên cứu ứng dụng công nghệ CRISPR/Cas9 chỉnh sửa promoter OsSWEET14 nhằm nâng cao tính kháng bệnh bạc lá ở giống lúa BT7" được hoàn thành với mục tiêu nhằm tối ưu quy trình chuyển gen vào phôi non (immature embryo – IE) giống lúa BT7 thông qua vi khuẩn Agrobacterium tumefaciens; Thiết kế cấu trúc T-DNA biểu hiện phức hệ CRISPR/Cas9 chỉnh sửa promoter OsSWEET14 của giống lúa BT7 (gọi tắt là SW14-BT).
169p
minhquan0790
28-10-2023
21
10
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Luận án Tiến sĩ Nông nghiệp "Nghiên cứu ứng dụng công nghệ CRISPR/CAS9 chỉnh sửa promoter OsSWEET14 nhằm nâng cao tính kháng bệnh bạc lá ở giống lúa Bắc Thơm 7" trình bày các nội dung chính sau: Tối ưu quy trình chuyển gen vào phôi non (immature embryo – IE) giống lúa BT7 thông qua vi khuẩn Agrobacterium tumefaciens; Thiết kế cấu trúc T-DNA biểu hiện phức hệ CRISPR/Cas9 chỉnh sửa promoter OsSWEET14 của giống lúa BT7 (gọi tắt là SW14-BT); Sàng lọc dòng lúa BT7 chỉnh sửa SW14-BT tăng cường tính kháng với vi khuẩn Xoo Việt Nam.
169p
vimulcahy
02-10-2023
10
7
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Tóm tắt Luận án Tiến sĩ Nông nghiệp "Nghiên cứu ứng dụng công nghệ CRISPR/Cas9 chỉnh sửa promoter OsSWEET14 nhằm nâng cao tính kháng bạc lá ở giống lúa Bắc Thơm 7" được nghiên cứu nhằm mục tiêu: Tối ưu quy trình chuyển gen vào phôi non (immature embryo – IE) giống lúa BT7 thông qua Agrobacterium tumefaciens; Sàng lọc dòng lúa BT7 chỉnh sửa SW14-BT tăng cường tính kháng với vi khuẩn Xoo Việt Nam.
27p
vimulcahy
02-10-2023
6
4
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Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth.
11p
vihagrid
30-01-2023
6
3
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The highest significant values of total soluble sugars and their fractions were noted in explant stage. Embryogenic callus stage induced a significant increase in total soluble sugars, free amino acids, phenols and indoles concentrations as compared to callus and mature somatic embryo.
8p
schindler
30-12-2021
13
0
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Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz.
9p
hanthienngao
30-11-2021
9
1
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The liver is mainly hematopoietic in the embryo, and converts into a major metabolic organ in the adult. Therefore, it is intensively remodeled after birth to adapt and perform adult functions. Long non-coding RNAs (lncRNAs) are involved in organ development and cell differentiation, likely they have potential roles in regulating postnatal liver development.
13p
vibeauty
23-10-2021
14
1
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After a short gestation, marsupials give birth to immature neonates with lungs that are not fully developed and in early life the neonate partially relies on gas exchange through the skin. Therefore, significant lung development occurs after birth in marsupials in contrast to eutherian mammals such as humans and mice where lung development occurs predominantly in the embryo.
14p
vitzuyu2711
29-09-2021
6
1
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Barley is known to be recalcitrant to tissue culture, which hinders genetic transformation and its biotechnological application. To date, the ideal explant for transformation remains limited to immature embryos; the mechanism underlying embryonic callus formation is elusive.
19p
vijichea2711
28-05-2021
10
1
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The most important factor for the success of plantation is readily availability of quality seeds. In favorable climatic conditions with optimum moisture and temperature most of the tree species germinate quickly and in unfavorable conditions some degree of seed dormancy occurs.
16p
trinhthamhodang9
16-12-2020
11
2
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Success rate in selection of putative transformants is mainly dependent upon the optimum concentration of selective agent. Effect of antibiotic concentrations of hygromycin (10, 20, 30 and 50 mg/L) and paromomycin (10, 20, 30 and 50 mg/L) in callusing and regeneration media on immature embryo-derived calli was evaluated using wheat variety PBW621.
8p
chauchaungayxua8
03-10-2020
13
2
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Aromatic rice is profitable farming venture and having high consumer demand. The main concerns about aromatic rice are low yield and susceptibility to pests and diseases. A rapid and reproducible genetic transformation protocol was optimized for three aromatic rice varieties using the established plant regeneration protocol.
30p
nguaconbaynhay6
23-06-2020
11
1
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In Quercus suber, cork oak, a Mediterranean forest tree of economic and social interest, rapid production of isogenic lines and clonal propagation of elite genotypes have been achieved by developing in vitro embryogenesis from microspores and zygotic embryos respectively.
18p
viminato2711
22-05-2020
11
0
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Microspore embryogenesis describes a stress-induced reprogramming of immature male plant gametophytes to develop into embryo-like structures, which can be regenerated into doubled haploid plants after whole genome reduplication. This mechanism is of high interest for both research as well as plant breeding.
16p
vihashirama2711
21-05-2020
14
1
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The relatively low efficiency of biolistic transformation and subsequent integration of multiple copies of the introduced gene/s significantly complicate the genetic modification of wheat (Triticum aestivum) and other plant species.
8p
vichoji2711
29-04-2020
9
1
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Domesticated einkorn (Triticum monococcum L.) is one of the oldest cultivated cereal crops in the world. Its small genome size (~ 5.7 GB), low ploidy (2n = 2x = 14, AmAm) and high genetic polymorphism make this species very attractive for use as a diploid model for understanding the genomics and proteomics of Triticeae.
13p
vihamax2711
21-04-2020
11
0
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Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana „Virupakshi‟. Immature male flower bud explant was used for embryogenic callus induction.
8p
chauchaungayxua3
07-02-2020
15
2
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The CRISPR/Cas9 system has been used predominantly for precise editing of the plant genomes. In the present study, we have made an application of CRISPR/Cas9 system for targeted mutagenesis in rice(Oryza sativa L.) targeting the Phytochrome and Flowering Time 1 (PFT1) gene known to be involved in disease susceptibility in Arabidopsis for rootinfecting hemibiotrophic fungal pathogen Fusarium oxysporum. Hence, to confirm the role of OsPFT1 gene in rice sheath blight disease, CRISPR-Cas9 gene editing tool is being employed for generating OsPFT1 gene knock out in rice.
9p
chauchaungayxua3
07-02-2020
8
0
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Hox genes regulate organ formation and identity of the embryo, and expressed in specific temporo-spatial patterns in the developing embryo. We compared the expression levels of the Hoxa5, Hoxb5, surfactant protein (SP)-A, and SP-B genes in immature and mature rabbit fetal lung tissues, and to uncover roles for Hoxa5, Hoxb5, SP-A, and SP-B.
8p
vicaracas2711
27-11-2019
16
0
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Somatic embryogenesis was initiated from immature zygotic embryos of Abies cilicica Carr. and its hybrid A. cilicica x A. nordmanniana. Schenk and Hildebrandt medium (SH) supplemented with 5 µM benzylaminopurine was used as the initiation medium.
6p
viminotaur2711
30-10-2019
14
0
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