In vitro culture

In this study, stem nodes with dorminant shoots were cultured on different media after selecting an optimized medium from experiments supplemented with BA in combination with IBA with different concentrations.

6p vifilm 11-10-2024 2 1   Download

Seed propagation of Mycetia balansae Drake is not easy, due to low germination rate and low viability, so it is very rare in nature. In order to conserve this plant as well as to use for commercial purposes in cooperation with Tue Linh Co.Ltd, providing a large number of plants is necessary and in vitro tissue culture technology could be solve this problem. Therefore, the study on "Callus induction and plant regeneration in vitro from leaf explants of mycetia balansae drake" was carried out.

8p xuanphongdacy09 29-09-2024 3 1   Download

This study was carried out to evaluate the effect of Cobalt chloride (CoCl2) on cardiac mitochondrial function in an in vitro model. In the study, H9C2 cardiomyocytes were cultured in a medium containing different concentrations of CoCl2. Cell viability, cardiolipin content, mitochondrial function, and mitochondrial oxidative stress were assessed by using Cell Counting Kit-8 and suitable fluorescence kits.

6p guitaracoustic02 08-12-2021 5 2   Download

Objectives of the study: Survey of biological activity of alkaloid isolated from hairy roots of E.longifolia to find new activities. Determining optimal culture conditions for E.longifolia hairy roots to grow and develop. Determining the concentration of three elicitors (Salicylic acid, Jasmonic acid and Yeat extract) on the production and accumulation of alkaloids isolated from hairy roots of E.longifolia in vitro.

26p extraenglish 24-05-2021 30 5   Download

Optimazize the in vitro Ngoc Linh ginseng lateral root culture and multiplicate the root biomass is more stable and higher in bioreactor system. Determine and evalute the effect of different types, and concentrations of five elicitors (SA, JA, ABA, CHN và YE) on producing saponin compounds. Next, combine in various types of elicitors to enhance effectiveness of the saponin accumulation, and determine the best elicitation time to develop the highest saponin in LRs of Ngoc Linh từ ARs in vitro.

27p nguathienthan12 23-05-2021 16 3   Download

The objective of the topic: The objective of this project is determination the role of a number of factors such as LED light, type and concentration of sugar, plant growth regulators, mineral medium, amino acids and polyamine in the flowering of T.fournieri in vitro.

27p extraenglish 24-05-2021 31 5   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p capheviahe27 23-02-2021 21 5   Download

The objective of this project is determination the role of a number of factors such as LED light, type and concentration of sugar, plant growth regulators, mineral medium, amino acids and polyamine in the flowering of T. fournieri in vitro.

27p larachdumlanat129 20-01-2021 35 5   Download

In this study, we investigated four factors to improve the hairy root induction in Urena lobata L. These factors include: age of plant (15-day-old in vitro plants, 45-day-old in vitro plants and after two subculture generations plants), different parts of plant (roots, stems, and leaves), infection time (10, 20 and 30 minutes), and culture medium (Murashige and Skoog (MS), Gamborg B5 medium (GB5) and Woody plant medium (WPM)).

8p larachdumlanat127 02-01-2021 6 2   Download

In order to set up a system for production of recombinant proteins using suspension cell culture of Nicotiana tabacum cv. Bright Yellow 2 (BY-2), in vitro culture and genetic transformation protocols of such cells were optimised at the laboratory of Plant Cell Biotechnology, Institute of Biotechnology.

10p tamynhan8 04-11-2020 9 1   Download

Với các ưu điểm vượt trội của cây Keo lai so với các loài cây lâm nghiệp khác mà hiện nay loài cây này chiếm phần lớn trên diện tích trồng rừng của Việt Nam. Từ đó nhu cầu về giống cho trồng rừng là vô cùng lớn. Tuy nhiên việc nhân giống bằng các phương pháp truyền thống như giâm hom, gieo hạt không đáp ứng được nhu cầu về cây giống sạch bệnh, đồng đều về phẩm chất. Cùng với những kết quả về cải thiện giống, công nghệ nhân giống bằng phương pháp nuôi cấy mô (tissue culture) được xem là giải pháp công nghệ hàng đầu để duy trì chất lượng di truyền của cây giống.

7p cothumenhmong8 05-11-2020 36 3   Download

Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium.

13p kequaidan7 01-09-2020 15 3   Download

To identify species, biological traits, chemical composition, and bioactive activities of essential oil and to develop propagation technical process using tissue culture techniques from shoot bud cutting to contribute to the conservation and development of genetic sources of native ginger plants in Bac Kan.

27p trinhthamhodang7 31-08-2020 45 2   Download

Three bio-agents i.e. Trichoderma viridae, Trichoderma harzianum and Trichoderma aspergillum were evaluated against Botrytis cinerea under in vitro condition. Culture disc of fungal bio-agents and pathogen were taken from the growing cultures and transferred to PDA contained in 70 mm petridishes on opposite sides approximately at one cm from the wall of the plate. Among these three bio-gents Trichodema harzianum showed best mycelial growth inhibition of Botrytis cinerea.

5p nguaconbaynhay7 15-08-2020 14 2   Download

Foot rot of tomato is an important disease which causes severe loss in tomato production. The present study was carried out to evaluate five bioagents and six fungicides against Sclerotium rolfsii. Among the five antagonists tested the maximum inhibition was observed in Trichoderma virens (67%) and contrarily, no inhibition was observed in Bacillus subtilis. Six fungicides viz., carbendazim, captan, tebuconozole, copper oxychloride, hexaconozole and Tebuconazole +Trifloxytrobin at different concentrations (50, 100, 150, 200, 250 and 500 ppm), were tested.

8p chauchaungayxua6 26-06-2020 18 4   Download

Among the diseases of safflower; dry root rot disease caused by Macrophomina phaseolina (Tassi.) Goid is one of the most destructive and wide spread disease of Safflower (Carthamous tinctorius L.) causing accountable losses of about 25-60 per cent. Eleven Botanicals evaluated in vitro were found fungistatic/antifungal against M. phaseolina. Amongs these A. sativum followed by A. cepa and V. negundo caused significantly highest mean mycelial inhibition 83.57, 72.09 and 65.85 per cent respectively.

15p gaocaolon5 14-06-2020 12 3   Download

An efficient lighting system (Compact 3U) was successfully applied to the micropropagation of gloxinia and potato plants. To compare the in vitro growth of plantlets under Neon and Compact 3U lighting systems, gloxinia and potato shoots were cultured on suitable media at different light intensities: (1) 45 µmol m-2s -1 (under Neon lighting system as a control); (2) 45 µmol m-2s -1, (3) 60 µmol m-2s -1, and (4) 75 µmol m-2s -1 (under Compact 3U lighting systems).

9p 12120609 01-06-2020 6 1   Download

Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations.

5p caygaocaolon4 04-04-2020 12 1   Download

The results showed that protocorms were formed from seeds culture on the ½ MS medium supplemented with 10% potatoes extract within 8 weeks. Protocorms which were cultured on the ½ MS medium containing 30 g/l sucrose, 0.5 g/l AC, 7 g/l agar, 0.1 mg/l NAA and 1 mg/l BA were optimal for PLB formation (8.67 PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, 0.5 g/l AC, and 7 g/l agar after about 90 days.

8p slimzslimz 08-12-2019 14 2   Download

General objective: To get mutant flowering flowers that have more number of petals than the study samples, larger flowers and more aromatic. To evaluate growth culture technique to generate calli and shoots for irradiation of gamma ray;

31p phongtitriet000 08-08-2019 33 4   Download