In vitro culture

Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance.

17p vishanshan 27-06-2024 1 1   Download

Ebook "Plant cell culture protocols (Third edition)" is taught around the world as part of undergraduate and graduate courses in agronomical and biological sciences. However, the apparent simplicity of cell culture technology should not lead to mistakingly consider it as trivial. It would be very diffi cult to understand modern plant biotechnology without in vitro cell cultures and even today, the application of recombinant DNA technology to important crops is hampered by the poor embryogenic or morphogenetic response of in vitro cultured tissues.

445p dongmelo 27-05-2024 5 2   Download

In this study, the effect of plant growth regulators (BA and NAA), ripe bananas in combination with potato, humic acid, and silver nanoparticles (AgNPs) were investigated to find a suitable condition for in vitro plant regeneration from R. imschootiana protocorm-like body (PLB). The results showed that after 45 days of culture, MS medium supplemented with 100 g ripe banana/liter and 100 g potato/liter was suitable for regenerating shoots from the protocorm-like body (PLB) (17.56 shoots/PLBs and 100.00% of shoot-regenerating PLBs).

13p dianmotminh02 03-05-2024 4 1   Download

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants. These were free-disease symptoms that were collected for surface sterilization.

8p vijaychest 24-04-2024 2 2   Download

The procedure reported in this article is useful for regeneration of drumstick plantlets from shoots which is a mean of propagation of plants with the identical genetic traits for field studies.

10p vilarry 01-04-2024 1 1   Download

Ebook "Plant-based remediation processes (Soil biology, Volume 35)" is on the recent advances of technologies using green plants for remediation of various metals and metalloids. Topics include biomonitoring of heavy metal pollution, amendments of higher uptake of toxic metals, transport of heavy metals in plants, and toxicity mechanisms. Further chapters discuss agro-technological methods for minimizing pollution while improving soil quality, transgenic approaches to heavy metal remediation and present protocols for metal remediation via in vitro root cultures.

304p manmanthanhla0201 26-02-2024 5 1   Download

Ebook "Studies on periodontal disease (Oxidative stress in applied basic research and clinical practice series)" comprise four parts. Part 1 provides basic sciences from the general to the particular. The findings of cell culture and animal models supply the mechanism between periodontal disease and oxidative stress. In part 2, human clinical studies are mainly addressed. The effects of periodontal therapy on circulating oxidative stress are also discussed. The relationships between periodontal disease and systemic diseases are examined in part 3.

368p manmanthanhla0201 26-02-2024 6 1   Download

Part 1 book "Biomedical tissue culture" includes content: Culture conditions and types of growth media for mammalian cells, cell handling and culture under controlled oxygen concentration, contamination of tissue cultures by mycoplasmas, isolation of breast cancer stem cells by single cell sorting, in vitro, tissue based models as a replacement for animal models in testing of drugs at the preclinical stages, use of cell culture to prove syncytial connection and fusion of neurons.

123p muasambanhan07 20-02-2024 3 0   Download

Part 2 book "Biomedical tissue culture" includes content: Placental structure and biological aspects of fetal membranes cultured in vitro; tissue development and mechanical property in the regenerated cartilage tissue; the art of animal cell culture for virus isolation; tissue based model of HCV replication as a replacement for animal models in drug testing; tissue culture to assess bacterial enteropathogenicity; viral DNA and CDNA array in the diagnosis of respiratory tract infections.

124p muasambanhan07 20-02-2024 2 0   Download

Ebook "Plant biotechnology for health: From secondary metabolites to molecular farming" emphasis will be put in the relevance of Plant Biotechnology for producing compounds of pharmaceutical and industrial relevance specifically the contribution of in vitro plant cell cultures for producing recombinant proteins (molecular farming) and compounds produced by plants useful for human and animal health (secondary metabolites) will be discussed.

174p nhanphanguyet 28-01-2024 3 2   Download

Ebook "Bioprocessing of plant in vitro systems" presents how plant in vitro technologies can overcome current limitations in the production of important plant-derived substances. It explains the advantages of plant in vitro technologies, notably the independence from climatic and soil conditions and the ability to synthesize diverse bioactive substances under controlled conditions.

591p lamquandat 28-12-2023 8 2   Download

The aim of the present study was to evaluate the effect of foetal calf serum (FCS) on the production of bovine embryo in vitro. We conducted two experiments: (1) to evaluate the effect of FCS on the production of bovine embryo in vitro and (2) to evaluate the effect of duration of FCS supplementation on the production of bovine embryo in vitro.

5p vimichaelfaraday 28-12-2023 3 2   Download

Ebook "Retinal gene therapy: Methods and protocols" describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies.

373p lucchinguyen 28-12-2023 9 2   Download

Ebook "Hepatitis B virus: Methods and protocols" details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery.

301p lucchinguyen 28-12-2023 5 2   Download

Osteosarcoma (OS) mainly happens in children and youths. Surgery, radiotherapy and chemotherapy are the common therapies for osteosarcoma treatment but all their anti-tumor effects are limited.

12p vileonardodavinci 23-12-2023 5 4   Download

Recapitulating the tumor microenvironment (TME) in vitro remains a major hurdle in cancer research. In recent years, there have been significant strides in this area, particularly with the emergence of 3D spheroids as a model system for drug screening and therapeutics development for solid tumors. However, incorporating macrophages into these spheroid cultures poses specific challenges due to the intricate interactions between macrophages and cancer cells.

17p vileonardodavinci 23-12-2023 4 2   Download

Part 2 book "Animal models of epilepsy, methods and innovations" includes content: Imaging seizure propagation in vitro, complexity untangled - large scale realistic computational models in epilepsy, organotypic hippocampal slice cultures as a model of limbic epileptogenesis, seizure analysis and detection in vivo,.... and other contents.

129p oursky06 13-10-2023 6 2   Download

Part 1 book "Oocyte physiology and development in domestic animals" includes content: Regulation of oocyte meiotic resumption by somatic cells; oocyte development before and during folliculogenesi; the in vitro culture of ovarian follicles - a brief history and current considerations; oocyte secreted factors in domestic animals, MicroRNAs in oocyte physiology and development,.... and other contents.

129p oursky06 17-10-2023 2 2   Download

Part 1 book "Immunobiology of the shark" includes content: Shark complement - genes, proteins and function; MHC molecules of cartilaginous fishes; shark immunoglobulin light chains, shark T-cell receptors; the shark family (cartilaginous fish) immunogenome; in vitro culture of elasmobranch cells,...and other contents.

161p oursky05 20-09-2023 4 1   Download

Part 1 book "Animal cell technology, from biopharmaceuticals to gene therapy" includes content: Introduction to animal cell technology, animal cells - basic concepts, cloning and expression of heterologous proteins in animal cells, cell metabolism and its control in culture, culture media for animal cells, post-translational modification of recombinant proteins, mechanisms of cell proliferation and cell death in animal cell culture in vitro, mathematical models for growth and product synthesis in animal cell culture.

259p oursky05 20-09-2023 6 2   Download