In vitro cytotoxicity

New ruthenium(III) complexes with 3-(Benzothiazol-2-yliminomethyl)-phenol (HL) ligand have been synthesized and characterized with the aid of elemental analysis, IR, electronic, and electron paramagnetic resonance spectroscopic techniques.

12p tocectocec 25-05-2020 12 3   Download

The selectivity index of these compounds is similar to that of cidofovir. Although antiviral activity was evident in secondary assays, significant virus inhibition occurred at or near the cytotoxic concentration (SI90=1).

8p tocectocec 25-05-2020 12 1   Download

Eosinophil cationic protein (ECP), one of the major components of basic granules of eosinophils, is cytotoxic to tracheal epithelium. However, the extent of this e€ect on other cell types has not been evaluatedin vitro.Inthis study, we evaluated the e€ect of ECP on 13 mammalian cell lines. ECP inhibited the growth of several cell lines including those derived from carcinoma and leukemia in a dose-dependent manner.

10p research12 29-04-2013 36 4   Download

p-Hydroxyphenylacetate decarboxylase from Clostridium difficilecatalyses the decarboxylation ofp-hydroxyphenyl-acetate to yield the cytotoxic compoundp-cresol. The three genes encoding two subunits of the glycyl-radical enzyme and the activating enzyme have been cloned and expressed in Escherichia coli. The recombinant enzymes were used to reconstitute a catalytically functional system in vitro.In contrast with the decarboxylase purified fromC.

6p dell39 03-04-2013 43 4   Download

Chronic obstructive pulmonary disease is a major clinical disorder usually associated with cigarette smoking. A central feature of chronic obstructive pulmonary disease is inflammation coexisting with an abnormal protease/anti-protease balance, leading to apoptosis and elastolysis. In anin vitro study of rat lung alveolar L2 cells, cigarette smoke extract (CSE) induced apoptotic cell death. Expo-sure of L2 cells to CSE at a concentration of 0.25% resulted in a 50% increase of caspase-3 and matrix met-alloproteinase (MMP) activities.

11p dell39 03-04-2013 53 3   Download

Human amylin is a small fibrillogenic protein that is the major constituent of pancreatic islet amyloid, which occurs in most subjects with type 2 dia-betes. There is evidence that it can elicitin vitro apoptosis in isletb-cells, but the physical properties that underpin its cytotoxicity have not been clearly elucidated.

11p inspiron33 25-03-2013 42 5   Download

Protein oligomeric complexes have emerged as a major target of current research because of their key role in aggregation processes in living systems andin vitro. Hydrophobic and charged surfaces may favour the self-assembly process by recruiting proteins and modifying their interactions. We found that equine lysozyme assembles into multimeric complexes with oleic acid (ELOA) at the solid–liquid interface within an ion-exchange chromatography column preconditioned with oleic acid.

15p vinaphone15 25-02-2013 51 4   Download

Onconase  (ONC) is a member of the ribonuclease A superfamily that is toxic to cancer cells in vitro andin vivo. ONC is now in Phase IIIb clinical trials for the treatment of malignant mesothelioma. Internalization of ONC to the cytosol of cancer cells is essential for its cytotoxic activity, despite the apparent absence of a cell-surface receptor protein.

12p vinaphone15 25-02-2013 30 3   Download

To assessPseudomonasexotoxin A (ETA) compartmentalization, process-ing and cytotoxicity in vivo, we have studied the fate of internalized ETA with the use of thein vivo rodent liver model following toxin administra-tion, cell-free hepatic endosomes, and pure in vitro protease assays. ETA taken up into rat liver in vivo was rapidly associated with plasma mem-branes (5–30 min), internalized within endosomes (15–60 min), and later translocated into the cytosolic compartment (30–90 min).

15p viettel02 19-02-2013 36 3   Download

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài :In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells

19p dauphong3 03-01-2012 52 6   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo...

11p toshiba14 22-10-2011 59 4   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: An ultrastructural study on cytotoxic effects of mono(2-ethylhexyl) phthalate (MEHP) on testes in Shiba goat in vitro

6p hoami_266 16-09-2011 47 4   Download