Báo cáo khoa học: Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase
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p-Hydroxyphenylacetate decarboxylase from Clostridium difficilecatalyses the decarboxylation ofp-hydroxyphenyl-acetate to yield the cytotoxic compoundp-cresol. The three genes encoding two subunits of the glycyl-radical enzyme and the activating enzyme have been cloned and expressed in Escherichia coli. The recombinant enzymes were used to reconstitute a catalytically functional system in vitro.In contrast with the decarboxylase purified fromC. difficile, whichwas an almost inactive homo-dimeric protein (b2), the recombinant enzyme was a hetero-octameric (b4c4 ), cata-lytically competent complex, ...
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