Inverse PCR

(BQ) Ebook Plant Reverse Genetics: Methods and Protocols – Part 2 presents the following content: Chapter 10: methods for rice phenomics studies; chapter 11 : development of an efficient inverse pcr method for isolating gene tags from t-dna insertional mutants in rice; chapter 12: transposon insertional mutagenesis in rice; chapter 13: reverse genetics in medicago truncatula using tnt1 insertion mutants; chapter 14: screening arabidopsis genotypes for drought stress resistance; chapter 15 : protein tagging for chromatin immunoprecipitation from arabidopsis.

151p runordie7 30-08-2022 6 2   Download

The expressions of Prunus dulcis C-repeat-binding factors (PdCBF1) and (PdCBF2) genes that are the major components in the cold responsive network of plants were studied in almonds. Real time PCR analysis of buds revealed the differential expression pattern of PpFT, PabSOC1, PdCBF1, PdCBF2 genes. The expressions of PpFT and PabSOC1 correlated with each other. Similarly, the expressions of PdCBF1 and PdCBF2 genes revealed a similar expression pattern in almonds. However, the expression of flowering genes were inversely correlated with the cold response genes in most of the almond accessions.

11p hanthienngao 30-11-2021 5 1   Download

Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations.

10p vibeauty 23-10-2021 13 0   Download

The genetic relationship among 14 spatially segregated populations of Karnataka and one each population from Kerala and Gujarat was established using polymerase chain reaction and arbitrary primers. A total of 24 random decamer DNA primers belonging A, B, C, G, H, I and ‘L’ series were used for RAPD-PCR. The lowest genetic similarity was noticed between the farthest populations and highest between the closest populations. Higher similarity index values were found among the populations within Karnataka. Pooled data revealed the lowest genetic similarity index of 0.

13p nguaconbaynhay1 04-12-2019 12 1   Download

Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated.

8p caplock2711 19-02-2019 28 0   Download

Đề tài này được tiến hành với mục tiêu nhằm xác định đột biến đảo đoạn intron 22 ở bệnh nhân hemophilia A thể nặng và người nữ dị hợp tử trong gia đình bệnh nhân. Mời các bạn cùng tham khảo bài viết để nắm rõ nội dung chi tiết của đề tài nghiên cứu này.

6p hanh_tv13 24-01-2019 61 2   Download

Bài viết Xác định đột biến đảo đoạn Intron 22 trên bệnh nhân Hemophillia A bằng kỹ thuật Inversion PCR trình bày bệnh Hemophilia A là bệnh rối loạn đông máu, di truyền lặn trên nhiễm sắc thể giới tính X, tần suất mắc bệnh là 1/5000 trẻ trai. Ở bệnh nhân Hemophilia A thể nặng, nồng độ protein yếu tố VIII trong máu rất thấp, chỉ ≤ 1% so với người bình thường, gây nên các biến chứng chảy máu nặng nề trong ổ khớp, cơ hay cơ quan nội tạng,... Mời các bạn cùng tham khảo.

7p baoyencute 11-05-2018 114 3   Download

uC31 integrase, a site-specific recombinase, can effectively mediate foreign genes bearing anattBsequence integrated into pseudoattPsites. We have previously identified two pseudoattP sites, BpsF1 and BpsM1 from the bovine genome. In this study, two new pseudoattPsites, BF4 and BF10, were discovered using half-nested inverse PCR from cow fibroblasts.

9p vinaphone15 27-02-2013 47 1   Download

(anchored PCR), kỹ thuật này yêu cầu chỉ cần biết trình tự nucleotide ở một đầu của khuôn mẫu và gắn một đuôi đồng trùng hợp nhân tạo (ví dụ: dA) kia (chưa biết trình tự). Sau đó đoạn oligo(dT) (Hình 3.5). (inverse PCR), đ với đoạn hạn chế nhờ enzyme DNA ligase đoạn đã 3.6).

18p iiduongii9 09-05-2011 103 16   Download

Assessment of Glomerular Filtration Rate Monitoring the GFR is important in both the hospital and outpatient settings, and several different methodologies are available (discussed below). In most acute clinical circumstances a measured GFR is not available, and the serum creatinine level is used to estimate the GFR in order to supply appropriate doses of renally excreted drugs and to follow short-term changes in GFR. Serum creatinine is the most widely used marker for GFR, and the GFR is related directly to the urine creatinine excretion and inversely to the serum creatinine (U Cr/PCr).

5p ongxaemnumber1 29-11-2010 77 3   Download