Isolator system

This study was carried out to provide scientific proof to justify the intended medicinal use of PDDT tablets with two main objectives: 1) Investigate the effects of PDDT on mice’ intestinal motility; 2) Evaluate the effects of PDDT on rabbits’ isolated intestinal segments.

7p viyamanaka 06-02-2025 2 2   Download

The authors develop a system of differential equations describing the motion of the structural system equipped with DFP bearings. The Runge-Kutta numerical method, specifically the ode15s function in MATLAB, was used to solve these equations. The results of the calculations provide a comparison of the damping efficiency of the structural system with and without the use of DFP bearings, assessing the effectiveness of vibration isolation during an earthquake, with three components X, Y, and vertical considered.

5p vibenya 31-12-2024 9 2   Download

Interface converter, for converting RS-232 to RS-422 and RS-485, with electrical isolation, 2 channels Bidirectional, wireless, 2400 MHz transmission system (European version), consisting of 2 transceivers (transmitter and receiver) and two antennas with connecting cable Fieldline Modular Wireless IO set, consisting of: Wireless IO base station for one Wireless IO terminal and Wireless IO device, 8 digital inputs, 8 digital outputs

23p buisontdtq 04-10-2012 53 9   Download

In the course of current research the procedure of obtaining of the piecewise linear vibration systems time and frequency responses have been developed. The method is partly analytical and partly numerical and is based on combining of linear solutions of different sections in a cycle of motion. In a row with the method of obtaining of the exact steady state time and frequency response, also it is provided sensitivity analysis of the vibration isolator.

86p runthenight07 01-03-2023 13 3   Download

The increasing use of Intelligent Transport System (ITS) can enable very close vehicle spacings which generally results in a net drag reduction for the resulting convoys. The majority of vehicle development has, to date, been for vehicles in isolation, thus the study of interaction effects is becoming increasingly important. The main objective of this research is to investigate the use of Computational Fluid Dynamics (CFD) for understanding convoy aerodynamics and to further the understanding of airflow interaction between vehicles via CFD.

154p runthenight07 01-03-2023 10 3   Download

The objectives of the thesis: Identify the pathogenicity potential and classificate at species level of Colletotrichum spp. isolated from hot chilli in Vietnam; produce stable oligochitosan-silica nano, which potentially highly induced effectively the systemic resistance against anthranose disease on hot chilli, based on the combination of low molecular weight chitosan with nano silica.

28p capheviahe27 23-02-2021 35 4   Download

When a satellite is in orbit, micro vibration generated by its actuators (such as reaction wheels, deployable mechanisms, etc.) will affect the imaging quality of the camera. Viscoelastic materials are utilized as passive isolators to reduce these micro vibrations due to their simple construction and excellent energy dissipation capacity.

8p tohitohi 19-05-2020 16 1   Download

From the antennae of the moth Mamestra brassicae, we have identified a lepidopteran G protein a subunit belonging to the Gq family, through immunological detection in crude antennal extract and antennal primary cell cultures, followed by molecular cloning. The complete cDNA sequence (1540 bp) contains an open reading frame encoding a protein of 353 amino acids.

10p research12 01-06-2013 26 4   Download

Cytochrome P450foxy (P450foxy, CYP505) is a fused protein of cytochrome P450 (P450) and its reductase isolated from the fungus Fusarium oxysporum, which catalyzes the subterminal (x-1x-3) hydroxylation of fatty acids. Here, we produced, purified and characterized a fused recombinant protein (rP450foxy) using the Escherichia coli expression system. Purified rP450foxy was catalytically and spectrally indistinguishable from the native protein, but most of the rP450foxy was recovered in the soluble fraction of E. coli cells unlike the membrane-bound native protein....

8p research12 01-06-2013 60 4   Download

Random PCR mutagenesis was applied to theThermus thermophilus xylA gene encoding xylose isomerase. Three cold-adapted mutants were isolated with the following amino-acid substitutions: E372G, V379A (M-1021), E372G, F163L (M-1024) and E372G (M-1026). The wild-type and mutated xylAgenes were cloned and expressed in Escherichia coliHB101 using the vector pGEMÒ-T Easy, and their physicochemical and catalytic properties were determined.

7p research12 29-04-2013 55 3   Download

All living organisms contain redox systems involving thior-edoxins (Trx), proteins featuringanextremelyconservedand reactive active site that perform thiol-disul®de interchanges with disul®de bridges of target proteins. In photosynthetic organisms, numerous isoforms of Trx coexist, as revealedby sequencing ofArabidopsisgenome. The speci®c functions of many of them are still unknown.

11p research12 29-04-2013 35 2   Download

Xanthomonas campestrispv.vitians is a Gram-negative plant-associated bacterium that acts as causative agent of bacterial leaf spot and headrot in lettuce. The lipopolysac-charide of this bacterium is suspected to be an important molecule for adhesion to and infection of the plants. The lipopolysaccharide has been isolated from the phenol phase and the O-specific chain characterized by compositional analysis, high fieldNMRandMALDI-TOFMS.

9p research12 23-04-2013 45 2   Download

Plant cell wall proteins called expansins are thought to disrupt hydrogen bonding between cell wall polysaccha-rides without hydrolyzing them. We describe here a novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei. The pro-tein named swollenin has an N-terminal fungal type cel-lulose binding domain connected by a linker region to the expansin-like domain.

10p research12 23-04-2013 36 4   Download

RNA aptamers with anity for the Ras-binding domain (RBD) of Raf-1 were isolated from a degenerate pool by in vitroselection.These aptamers eciently inhibited theRas interaction with the Raf-1 RBD, and also inhibited Ras-induced Raf-1 activation in a cell-free system. The RNA aptamer with the most potent inhibitory e€ect speci®cally inhibited the RasáRaf-1 interaction and had no anity for the RBD of the RGL protein, a homolog of the Ral GDP dissociation stimulator.

8p research12 23-04-2013 40 3   Download

Cytochrome P450BM3is a self-sufficient fatty acid mono-oxygenase consisting of a diflavin (FAD/FMN) reductase domain and a heme domain fused together in a single polypeptide chain. The multidomain structure makes it an ideal model system for studying the mechanism of electron transfer and for understanding P450 systems in general. Here we report the redox properties of the cyto-chrome P450BM3 wild-type holoenzyme, and its isolated FADreductase andP450 heme domains, when immobilized in a didodecyldimethylammonium bromide film cast on an edge-plane graphite electrode....

7p tumor12 20-04-2013 51 3   Download

The cytochromec nitrite reductase is isolated from the membranes of the sulfate-reducing bacteriumDesulfovibrio desulfuricansATCC 27774 as a heterooligomeric complex composedby two subunits (61 kDa and19 kDa) containing c-type hemes, encoded by the genes nrfA and nrfH, respectively. The extracted complex has in average a 2NrfA:1NrfH composition. The separation of ccNiR sub-units from one another is accomplished by gel filtration chromatography in the presence of SDS.

12p tumor12 20-04-2013 41 3   Download

Liver microsomal preparations are routinely used to predict drug interactions that can occurin vivo as a result of inhi-bition of cytochrome P450 (CYP)-mediated metabolism. However, the concentration of free drug (substrate and inhibitor)at its intrahepatic site of action, a variable that cannot be directly measured, may be significantly different from that in microsomal incubation systems.

10p tumor12 20-04-2013 48 4   Download

Antimicrobial peptides form one of the first lines of defense against invading pathogens by killing the microorganisms and/ormobilizing the host innate immune system. Although over 800 antimicrobial peptides have been isolated from many different species, especially insects, few have been reported frommarinefish. Sequenceanalysisof twogenomic clones (15.6 and 12.5 kb) from the winter flounder, Pseudopleuronectes americanus(Walbaum) resulted in the identification of multiple clustered genes for novel pleuro-cidin-like antimicrobial peptides....

11p tumor12 20-04-2013 28 2   Download

Asheat shockproteins (Hsps) are involved inprotecting cells and also in the pathophysiology of diseases such as inflam-mation, cancer and neurodegenerative disorders, modula-tors ofHsp expression inmammalian cells would seemto be useful for the treatment of various diseases. In this study, we isolated mammalian cell lines for screening of Hsp modu-lators; mouse C3H10T1/2 cells stably transfected with a plasmid containing the mouse Hsp105 or human Hsp70B promoter upstream of a luciferase or b-galactosidase reporter gene, respectively....

8p tumor12 20-04-2013 33 5   Download

NAD-dependent formate dehydrogenase (FDH1) was isolated from the a-proteobacterium Methylobacterium extorquensAM1 under oxic conditions. The enzyme was found to be a heterodimer of two subunits (a1b1)of107 and 61 kDa, respectively. The purified enzyme contained per mol enzyme
5 mol nonheme iron and acid-labile sulfur, 0.6 mol noncovalently bound FMN, and
1.8 mol tungsten.

9p tumor12 20-04-2013 31 3   Download