Kinetic parameters

This thesis derives closed form equations to describe electromagnetic device characteristics for a toroid, solenoid and a case study induction machine. By considering stored magnetic field energy, this approach allows the relationship between electrical, magnetic and kinetic energy to be quantified and analyzed in ways that allows improved accuracy compared with conventional magnetic circuit modeling methods. This thesis also provides insights into how geometric parameters impact energy transfer and operational characteristics of electromagnetic devices.

112p runthenight04 02-02-2023 6 2   Download

Building a model, setting up a system of dynamic equations of the circular saw cutting bamboo, solving the dynamic equations, from which to calculate and determine some reasonable kinetic and dynamic parameters of the circular saw to improve productivity and cutting surface quality

26p closefriend09 16-11-2021 12 4   Download

C2H5OH has been using as an alternative fuel for decades; HO2 also plays a pivotal role in the combustion. The kinetics and mechanism for the reaction between C2H5OH and HO2 radical have been investigated using the molecular parameters for the reactants, transition states and products predicted at the CCSD(T)//B3LYP/6-311++G(3df,2p) level of theory.

7p tamynhan6 14-09-2020 16 1   Download

Hepcidin (H25) is a hormone peptide synthesized by the liver that binds to ferroportin and blocks iron export. In this study, H25 was inhibited by administration of single and multiple doses of an anti-H25 monoclonal antibody Ab 12B9m in cynomolgus monkeys. The objective of this analysis was to develop a pharmacodynamic model describing the role of H25 in regulating iron homeostasis and the impact of hepcidin inhibition by Ab 12B9m. Total serum H25 and Ab 12B9m were determined in each animal. Corresponding measurements of serum iron and hemoglobin (Hb) were obtained.

15p caothientrangnguyen 09-05-2020 12 1   Download

Bài báo đã trình bày một số kết quả ban đầu về sự phụ thuộc các thông số trên vào liều chiếu và thời gian bảo quản. Phương pháp có thể sử dụng để nghiên cứu các mẫu thực phẩm khác và sử dụng để xác định liều chiếu các thực phẩm chiếu xạ. Mời các bạn cùng tham khảo.

8p bautroibinhyen16 09-02-2017 38 1   Download

Unstructured modelling growth of Lactobacillus acidophilus as a function of the temperature: We present modelling software developed under MATLAB in which parameter estimations are obtained by using non-linear regression techniques. The different parameters appear in a set of non-linear algebraic and differential equations representing the model of the process. From experimental data obtained in discontinuous cultures a representative mathematical model (unstructured kinetic model) of the macroscopic behaviour of Lactobacillus acidophilus has been developed.

9p nhoxtom 29-09-2015 58 5   Download

The gene fprA of Mycobacterium tuberculosis, encoding a putative protein with 40% identity to mammalian adrenodoxin reductase, was expressed in Escherichia coli and the protein purified to homogeneity. The 50-kDa protein monomer contained one tightly bound FAD, whose fluorescence was fully quenched. FprA showed a low ferric reductase activity, whereas it was very active as a NAD(P)H diaphorase with dyes. Kinetic parameters were determined and the specificity constant (kcat/Km) for NADPH was two orders of magnitude larger than that of NADH....

9p system191 01-06-2013 36 3   Download

Introducing site-directed mutations in surface-exposed residues of subunit II of the heme aa3 cytochrome c oxidase of Paracoccus denitrificans, we analyze the kinetic parameters of electron transfer from reduced horse heart cytochrome c. Specifically we address the following issues: (a) which residues on oxidase contribute to the docking site for cytochrome c, (b) is an aromatic side chain required for electron entry from cytochrome c, and (c) what is the molecular basis for the previously observed biphasic reaction kinetics....

9p system191 01-06-2013 36 3   Download

VanXYC, a bifunctional enzyme from VanC-phenotype Enterococcus gallinarum BM4174 that catalyses D,D-peptidase and D,D-carboxypeptidase activities, was purified as the native protein, as a maltose-binding protein fusion and with an N-terminal tag containing six histidine residues. The kinetic parameters of His6–VanXYC were measured for a variety of precursors of peptidoglycan synthesis involved in resistance: for D-Ala-D-Ala, the Km was 3.6 mM and kcat, 2.5 s)1; for UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-DAla (UDP-MurNAc-pentapeptide[Ala]), Km was 18.8 mM and kcat 6.

7p system191 01-06-2013 40 4   Download

Vanadium haloperoxidases and the bacterial class A nonspecific acid phosphatases have a conserved active site. It is shown that vanadate-substituted recombinant acid phosphatase from Shigella flexneri (PhoN-Sf) and Salmonella enterica ser. typhimurium (PhoN-Se) in the presence of H2O2 are able to oxidize bromide to hypobromous acid. Vanadate is essential for this activity. The kinetic parameters for the artificial bromoperoxidases have been determined. The Km value for H2O2 is about the same as that for the vanadium bromoperoxidases from the seaweed Ascophyllum nodosum. ...

6p research12 01-06-2013 29 4   Download

A comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal binding site of human and bovine serum albumins (HSA and BSA, respectively) and short peptide analogues was performed using potentiometry and spectroscopic techniques. It was found that while qualitative aspects of interaction (spectra and structures of complexes, order of reactions) could be reproduced, the quantitative parameters (stability and rate constants) could not.

9p research12 01-06-2013 38 4   Download

Arecombinant streptococcalC5apeptidasewas expressed in Escherichia coliand its catalytic properties and thermal sta-bility were subjected to examination. It was shown that the NH2 -terminal region ofC5a peptidase (Asn32–Asp79/ Lys90) forms the pro-sequence segment. Upon maturation the propeptide is hydrolyzed either via an autocatalytic intramolecular cleavage or by exogenous protease strepto-pain. At pH 7.4 the enzyme exhibited maximum activity in the narrow range oftemperatures between 40 and 43
C....

13p research12 29-04-2013 46 3   Download

Tightly coupled inside-out vesicles were prepared from Paracoccus denitrificanscells (SPP, sub-Paracoccusparticles) and characterized kinetically. The rate of NADHoxidation, catalysed by SPP, increases 6–8 times on addition of gram-icidin. The vesicles are capable of catalysing DlH + -dependent reverse electron transfer fromquinol toNAD + . The kinetic parameters of the NADH-oxidase and the reverse electron transfer carried out by membrane-bound P. denitrificanscomplex I were estimated and compared with those of the mitochondrial enzyme. ...

5p research12 23-04-2013 43 3   Download

The kinetics of the reversible thermal unfolding, irreversible thermal unfolding, and reductive unfolding processes of bovine pancreatic ribonuclease A (RNase A) were investi-gated in NaCl/Pi solutions. Image parameters including Shannon entropy, Hamming distance, mutual information and correlation coefficient were used in the analysis of the CD and 1D NMR spectra. The irreversible thermal unfolding transition of RNase A was not a cooperative process, pretransitional structure changes occur before the main thermal denaturation....

9p tumor12 22-04-2013 43 4   Download

The glyoxalase pathway ofLeishmania infantumwas kinetically character-ized as a trypanothione-dependent system. Using time course analysis based on parameter fitting with a genetic algorithm, kinetic parameters were estimated for both enzymes, with trypanothione derived substrates. A Kmof 0.253 mmand aVof 0.21lmolÆmin )1 Æmg )1 for glyoxalase I, and a Kmof 0.098 mmand aVof 0.18lmolÆmin )1 Æmg )1 for glyoxalase II, were obtained.

11p awards 06-04-2013 46 2   Download

Substitution oftrans-proline at three positions in ubiquitin (residues 19, 37 and 38) produces significant context-dependent effects on protein stability (both stabilizing and destabilizing) that reflect changes to a combination of parameters including backbone flexibility, hydrophobic interactions, solvent accessibility to polar groups and intrinsic backbone conformational preferences. Kinetic analysis of thewild-type yeast protein reveals a predominant fast-folding phase which conforms to an apparent two-state folding model. ...

11p awards 05-04-2013 49 4   Download

In this work we compared two plant ureases, jackbean urease (JBU) and embryo-specific soybean urease (SBU) and a bacterial (Bacillus pasteurii) urease, for kinetic parameters and other biological properties described recently for ureases that are independent of the ureolytic activity. The insecticidal effect of ureases was investigated in feedingtrials with the cotton sucker bug,Dysdercus peruvi-anus (Hemiptera) as an insect model. Contrastingwith B. pasteuriiurease (PBU), both plant ureases presented potent insecticidal activity, with LD50 values of 0.017% (w/w) and 0.

7p dell39 03-04-2013 43 2   Download

The control properties of biochemical pathways can be described by control coefficients and elasticities, as defined in the framework of metabolic control analysis. The determination of these parameters using the traditional metabolic control analysis relationships is, however, lim-ited by experimental difficulties (e.g. realizing and meas-uring small changes in biological systems) and lack of appropriate mathematical procedures (e.g. when the more practical large changes are made).

12p dell39 03-04-2013 62 3   Download

The enzymatic kinetics of glycoside hydrolase family 7 cellobiohydrolase (Cel7A) towards highly crystalline celluloses at the solid–liquid interface was evaluated by applying the novel concept of surface density (q) of the enzyme, which is defined as the amount of adsorbed enzyme divided by the maximum amount of adsorbed enzyme.

10p inspiron33 25-03-2013 58 4   Download

Thrombi, which are dissolved primarily by plasmin (EC 3.4.21.7.), contain up to millimolar concentrations of fatty acids and these are known to affect the action of the protease. In the present study the modulation of plasmin activity was characterized quantitatively in a continuous amidolytic assay based on synthetic plasmin substrate (Spectrozyme-PL).

9p media19 06-03-2013 41 5   Download