Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes
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We report the effects of stabilizing osmolytes (low molecular mass organic compounds that raise the midpoint of thermal denaturation) on the stabil-ity and function of RNase-A under physiological conditions (pH 6.0 and 25C). Measurements of Gibbs free energy change at 25C(DGD) and kinetic parameters, Michaelis constant (Km) and catalytic constant (kcat )of the enzyme mediated hydrolysis of cytidine monophosphate
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