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Lateral flow immunoassay test strip
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Among several methods to detect the toxic and cancerous aflatoxins in agricultural products used in foods and animal feeds, only the lateral flow immunodipstick method is suitable for field usage. The aim of this study is to determine optimal conditions for the conjugation of aflatoxin B1 (AFB1) with bovine serum albumin (BSA) to develop a lateral flow immunoassay test strip for detection of AFB1.
9p
viuzumaki2711
08-05-2019
13
0
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Lateral flow assay (LFA), or the immunochromatographic strip test, is popular to use for rapid and sensitive immunoassays. Gold nanoparticles (GNPs), due to tunable optical characteristics and easy manipulation of size or shape, represent an attractive approach for LFA technology.
15p
nutifooddau
27-01-2019
20
2
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This study was designed to construct a lateral flow immunoassay test strip for rapid detection of rubella virus. Rabbit anti-rubella antibody and pig anti-rubella antibody were successfully purified from sera of rabbit and pig infected with rubella virus. The yields of IgG affinity purification were 1.74 mg/ml and 3.23 mg/ml serum, respectively.
6p
trangcham1896
10-12-2018
40
1
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In the study, we showed the result of covalent conjugation of antirotavirus antibody and AuNP for generating a lateral flow immunoassay strip to detect rotavirus in fecal samples. The suitable conditions for coating polyethylene glycol (PEG) on the surface of AuNP were 10.0 M PEG for 3 hours at room temperature (25 oC). Optimized conditions for covalent conjugation of antibody and AuNP were pH 4.0, 0.1 g antibody/conjugate, 0.01 mM reactant EDC/NHS [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/(N-hydroxy sulfosuccinimide].
8p
cumeo3000
01-08-2018
40
0
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