Leaf explant

Seed propagation of Mycetia balansae Drake is not easy, due to low germination rate and low viability, so it is very rare in nature. In order to conserve this plant as well as to use for commercial purposes in cooperation with Tue Linh Co.Ltd, providing a large number of plants is necessary and in vitro tissue culture technology could be solve this problem. Therefore, the study on "Callus induction and plant regeneration in vitro from leaf explants of mycetia balansae drake" was carried out.

8p runordie2 07-06-2022 16 2   Download

Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz.

9p hanthienngao 30-11-2021 9 1   Download

An efficient regeneration protocol was established for a rare plant species, Begonia coptidifolia. Using leaf explants, adventitious shoots were induced on Murashige and Skoog (MS) basal medium using various concentrations (1.0–10.0 mM) of three cytokinins, 6-benzyladenine (BA), thidiazuron (TDZ) and kinetin (KIN). However, adventitious shoots could not be induced on MS medium supplemented with auxins [α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)] tested in the 1.0–10.0 mM range.

8p hanthienngao 30-11-2021 11 1   Download

The present study demonstrates a simple and efficient method for callus induction of Bamboo. The leaf, node and seed are used as explants for induction of callus. These explants were subjected to sterilization treatment and it is found that Tween -20, bavistin and ethanol are sufficient for proper sterilization of explants. Then these explants were subjected to various concentrations of growth hormone 2,4-Dichlorophenoxyacetic acid (2,4-D) added in Murashige and Skoog Media. It was concluded that the seeds are suitable explants for rapid induction of callus.

10p chauchaungayxua10 18-03-2021 7 1   Download

This paper focuses on researching of Spilanthes acmella (L.) Murr. callus culture. Different parts of the plant such as leaf, stem, flower and root have been cultured. Based on antimicrobial activity (5 - 20 mm on Staphylococcus and Vibrio) and radish root tip inhibition (82%), the callus derived from flowers is the best.

6p larachdumlanat127 20-12-2020 10 2   Download

This study presents mass multiplication system through callus mediated organogenesis in popular Anthurium andreanum cv. Tropical Red, Acropolis and Esmeralda.

6p caygaocaolon8 07-11-2020 7 1   Download

The present study was conducted during 2013- 2014 to find out the effect of induced mutation on in vivo growth parameters in „Camarosa‟ strawberry by following CRD factorial design. For the induction of variation explants (Runner tips, shoot tips and leaf disc) were subjected to different concentrations (0.1%, 0.2%, 0.3% and 0.4%) of EMS along with control for various treatment durations (1.5 hr, 2.5 hr and 3.5 hr).

12p angicungduoc8 07-11-2020 14 2   Download

The objective of the present investigation was to study the effect of hygromycin and cefotaxime on cultured tissues of lettuce and based on these results, an efficient in vitro selection system for the further transformation was developed.

12p chauchaungayxua8 03-10-2020 10 2   Download

The present investigation was carried out in Biotechnology Laboratory, Department of Olericulture, KAU, Vellanikkara with four genotypes of cucumber comprising gynoecious, monoecious and two parthenocarpic genotypes for in vitro plant regeneration. Seeds were grown in the culture bottles quarterly filled with half strength MS basal medium. Cotyledonary leaf explants were then transferred to eight media compositions of half strength MS medium with varying IAA and BAP concentrations in the culture test tubes.

10p chauchaungayxua8 03-10-2020 11 3   Download

The aim of this study was to develop an efficient protocol to standardize the concentration of sterilants for seed and explants sterilization for in vitro germination and regeneration respectively. The explants used are cotyledonary leaf, stem and hypocotyl of in vitro grown brinjal var.KKM1. For sterilization of seeds and explants, 5% sodium hypochlorite treatment for 3 minutes is the most effective sterilant concentration against the microbiological contamination. In subsequence, the regeneration protocol was optimised for the brinjal var.KKM1.

10p kethamoi7 26-08-2020 13 2   Download

In addition, the effects of explant age and lighting condition on shoot regeneration were also investigated. After 8 weeks of culture, the results showed that shoot regeneration rate (100%) and shoot multiplication coefficient (13.33) of the in vitro leaf-tTCL-4 were higher than those of other treatments and control. The shoot regeneration rate of P. edulis Sims. also varied with the change of explant age. The highest shoot regeneration rate (100%) was obtained from leaf explants of 1.5-month-old shoots after 8 weeks of culture.

9p nguyenxuankha_bevandan 14-08-2020 16 2   Download

Conventional propagation of Anthurium (Anthurium andraeanum [Lind.]) plant through seeds and suckers is a tedious and very slow process and not practical when carried out commercially on large scale. Hence, biotechnological approach such as micropropagation is considered as a novel method for production of pathogen-free true-to-type plantlets at a faster rate.

7p kethamoi5 03-06-2020 15 0   Download

Chrysanthemum is a second most important flower crop globally. Marigold is a introduced cultivar gaining popularity among farmers and consumers of southern part of India due to its colour and high shelf life. Due to its commercial importance as quality planting material and cut flowers it is difficult to mitigate the gradually increasing demand by only vegetative means of propagation which is time consuming with low multiplication rate. Plant tissue culture is a better alternative tool to overcome conventional limitations.

7p trinhthamhodang1213 27-05-2020 13 0   Download

Anthurium is an important ornamental plant grown around the globe and also in India. Seed and vegetative propagation of this plant are tedious and very slow process and not practical when carried out on a large scale. Therefore, biotechnological approach such as in- vitro cloning is found to be a novel method for production of pathogen free true-to-type plantlets at a faster rate. In this study, a competent surface sterilization procedure was standardized for aseptic in-vitro culture from leaf explants of Anthurium adraeanum (Hort.) cv. Fire under in vitro conditions.

7p trinhthamhodang5 16-05-2020 13 1   Download

The present experiment was conducted to find out the most suitable surface sterilant and timing for controlling contamination in the leaf segment explants of Ashoka (Saraca asoca Roxb. De Wilde) using MS as basal medium with BAP (2.0 mg/l) and NAA (0.5mg/l).

7p trinhthamhodang5 16-05-2020 14 1   Download

Unexpanded leaf rolls were used as explants for callus induction with different concentrations of mutagenic chemicals (sodium nitrite and ethylmethane sulfonate). The effect of these mutagenic chemicals on callusing, shooting and rooting was studied. Callus induction was observed in different concentrations of sodium nitrite (3 mg l-1 , 5 mg l-1 and 7 mg l-1 ) and EMS (0.6 µM, 0.8 µM, 1.0 µM) along with controls. Among all the treatments 3 mg l-1 (T4) SN and 0.6 µM EMS (T10) in 2009T5 were found to be the best for callus induction traits.

8p chauchaungayxua5 08-05-2020 17 2   Download

V. jatamansi Jones is an important medicinal wild herb of North Eastern Himalayan Region belonging to family Valerianaceae. The herb is widely used in treatment of leprosy, epilepsy, hysteria and asthma and also in making perfumed powder. Due to overexploitation of roots and rhizomes, V. jatamansi is included in endangered species as per National Medicinal Plant Board, New Delhi, India. The harvest of roots and rhizomes causes destruction of whole plant at its natural habitat.

7p kequaidan4 21-04-2020 11 0   Download

Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations.

5p caygaocaolon4 04-04-2020 12 1   Download

Regeneration protocol is pre-requisite for the development of transgenic plants. Maize (Zea mays L) crop is severely affected by fungal disease turcicum leaf blight. In the present study, standardizing the regeneration protocol for maize genotype was carried for the development of transgenic maize against turcicum leaf blight. The direct regeneration of maize involved nodal explants from the maize coleoptiles.

11p chauchaungayxua4 18-03-2020 10 0   Download

An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1 ) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1 . Rooting was initiated with full or half media supplemented with 0.

7p cothumenhmong3 22-02-2020 14 1   Download