Medical data

The role of management and the capability to examine and provide medical care is reflected in the availability of advanced diagnostic and treatment equipment, integrated with information technology systems to transmit data and images. This article focuses on analyzing the effects of science and technology, the digital revolution, and medical equipment on space organization for providing medical services in modern hospitals.

4p vibecca 01-10-2024 2 0   Download

The general aims of the present study are to determine the risks involved in acupuncture practice in Victoria, Australia as it is the first state that has systematically registered a non-medical associated treatment regiment and from an analysis of these risks, to develop an integrated framework for clinical risk management of acupuncture.

222p runthenight05 01-03-2023 7 3   Download

This study is attempted to determine the students’ passing ratio in English speaking skills and explore the effects of group work in teaching English speaking skills to general nurse students at DUMTP. 282 third-year students were selected to participate in the research. The collected data included scores of the pre- and post-treatment of oral tests and information from the questionnaires.

8p vivirginia2711 10-12-2020 54 5   Download

To evaluate the prognosis value of facial nerve conduction study in Bell’s palsy outcome. Subjects and methods: A descriptive and cross-sectional study using electro diagnostic data and medical chart review on 29 patients diagnosed with Bell’s palsy in Department of Neurology, Military Hospital 103 from January 2017 to December 2017, were evaluated using the facial nerve grading system 2.0 (FNGS) during their initial visit and on day 20 and day 40. We performed facial nerve conduction studies (NCS) in the first 5 days and on the 20th day.

5p chauchaungayxua1 02-12-2019 51 3   Download

Guanosine triphosphate nucleotide analogues such as GppNHp (also named GMPPNP) or GTPcS are widely used to stabilize rapidly hydrolyzing protein-nucleotide complexes and to investigate biochemical reaction pathways. Here we describe the chemical synthesis of guanosine 5¢-O-(c-amidotriphosphate) (GTPcNH2) and a new synthesis of guanosine 5¢-O-(c-fluorotriphosphate) (GTPcF). The two nucleotides were characterized using NMR spectroscopy and isothermal titration calorimetry. Chemical shift data on 31P, 19F and 1H NMR resonances are tabulated.

9p system191 01-06-2013 32 5   Download

Previous data showing an increase of receptor binding activity of [R16]VIP, a vasoactive intestinal peptide (VIP) structural analogue containing arginine at the position 16 of its amino acid sequence, have pointed out the importance of a positive charge at this site. Here, the functional characterization of three VIP polyaminated adducts (VIPDap, VIPSpd, and VIPSpm), obtained by a transglutaminasecatalysed reaction between the VIP Gln16 residue and 1,3-diaminopropane (Dap), spermidine (Spd), or spermine (Spm), is reported. ...

9p system191 01-06-2013 42 4   Download

In order to study some of its enzymatic properties, phosphatidylinositol synthase 1 (AtPIS1) from the plant Arabidopsis thaliana was expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novo synthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Our data clearly show that the CDP-diacylglycerol-independent exchange reaction can occur using endogenous PtdIns molecular species or PtdIns molecular species from soybean added exogenously....

6p research12 01-06-2013 33 5   Download

Obesity is a central feature of the metabolic syndrome and is associated with increased risk for insulin resistance and type II diabetes. Here, we investigated the contribution of human apoliprotein E3 and mouse apoli-protein E to the development of diet-induced obesity in response to western-type diet. Our data show that apolipoprotein E contributes to the development of obesity and other related metabolic disorders, and that human apolipoprotein E3 is more potent than mouse apolipoprotein E in promoting obesity in response to western-type diet. ...

14p research12 29-04-2013 46 3   Download

The complete coding sequence of a BDIX rat gene homo-logous to the human ABO gene was determined. Identifi-cation of the exon–intron boundaries, obtained by comparison of the coding sequence with rat genomic sequences from data banks, revealed that the rat gene structure is identical to that of the human ABO gene. It localizes to rat chromosome 3 (q11-q12), a region homolo-gous to human 9q34.

8p research12 23-04-2013 39 2   Download

Metabolic fluxes provide a detailed metric of the cellular metabolic phenotype. Fluxes are estimated indirectly from available measurements and various methods have been developed for this purpose. Of particular interest are meth-ods making use of stable isotopic tracers as they enable the estimation of fluxes at a high resolution. In this paper, we present data validating the use of mass spectrometry (MS) for the quantification of complex metabolic flux networks.

18p tumor12 20-04-2013 44 2   Download

Even though more than 200 three-dimensional structures of HIV-1 protease complexed to a variety of inhibitors are available in the Protein Data Bank; very few struc-tures of unliganded protein have been determined. We have recently solved structures of unliganded HIV-1 protease tethered dimer mutants to resolutions of 1.9 A˚ and 2.1 A ˚ , and have found that the flaps assume closed-flap conformation even in the absence of any bound lig-and.

9p tumor12 20-04-2013 21 2   Download

The process of pressure-induced modification of horse liver alcohol dehydrogenase (HLADH) was followed by measuringin situ catalytic activity (up to 250 MPa), intrinsic fluorescence (0.1–600 MPa) and modifications of FTIR spectra (up to 1000 MPa). The tryptophan fluor-escence measurements and the kinetic data indicated that the pressure-induced denaturation of HLADH was a process involving several transitions and that the observed transient states have characteristic properties of molten globules. ...

10p tumor12 20-04-2013 22 2   Download

The O-specific polysaccharide ofCitrobacter braakiiPCM 1531 (serogroupO6) was isolated bymild acid hydrolysis of the lipopolysaccharide (LPS) and found to containD-fucose, L-rhamnose, 4-deoxy-D-arabino-hexose andO-acetyl groups in molar ratios 2 : 1 : 1 : 1. On the basis of methylation analysis and 1 Hand13 C NMR spectroscopy data, the structure of the branched tetrasaccharide repeating unit of theO-specific polysaccharidewas established.

7p fptmusic 16-04-2013 51 4   Download

As a first step toward developing a zebrafish model for investigating the role of sulfation in counteracting environ-mental estrogenic chemicals, we have embarked on the identification and characterization of cytosolic sulfotrans-ferases (STs) in zebrafish. By searching the zebrafish expressed sequence tag database, we have identified two cDNA clones encoding putative cytosolic STs. These two zebrafish ST cDNAs were isolated and subjected to nuc-leotide sequencing. Sequence data revealed that the two zebrafishSTs are highlyhomologous, being
82%identical in their amino acid sequences....

8p fptmusic 16-04-2013 40 2   Download

The availability of extensive genomic information and content has spawned an era of high-throughput screening that is generating large sets of func-tional genomic data. In particular, the need to understand the biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins.

14p fptmusic 12-04-2013 29 2   Download

Heteronuclear NMR relaxation measurements and hydro-gen exchange data have been used to characterize protein dynamics in the presence or absence of stabilizing solutes from hyperthermophiles. Rubredoxin from Desulfovibrio gigaswas selected as a model protein and the effect of diglycerol phosphate on its dynamic behaviour was studied. The presence of 100 mMdiglycerol phosphate induces a fourfold increase in the half-life for thermal denaturation ofD.

9p fptmusic 12-04-2013 38 3   Download

Due to the unavoidable nonbiological variations accompanying many experiments, it is imperative to consider a way of unravelling the functional interaction structure of a cellular network (e.g. signalling cascades or gene networks) by using the qualitative information of time-series experimental data instead of computation through the measured absolute values.

10p fptmusic 11-04-2013 49 3   Download

A wide range of approaches has been applied to examine the quaternary structure of G protein-coupled receptors, the basis of such protein–protein interactions and how such interactions might modulate the pharmacology and function of these receptors. These include coimmunoprecipitation, var-ious adaptations of resonance energy transfer techniques, functional com-plementation studies and the analysis of ligand-binding data.

12p fptmusic 11-04-2013 49 3   Download

A yeast model was generated to study the mechanisms and phenotypical repercussions of expression of a-synuclein as well as the coexpression of protein tau. The data show that aggregation ofa-synuclein is a nucleation– elongation process initiated at the plasma membrane. Aggregation is con-sistently enhanced by dimethyl sulfoxide, which is known to increase the level of phospholipids and membranes in yeast cells.

15p awards 06-04-2013 40 2   Download

With the amount of genetic information available, a lot of attention has focused on systems biology, in particular biomolecular interactions. Con-sidering the huge number of such interactions, and their often weak and transient nature, conventional experimental methods such as X-ray crystal-lography and NMR spectroscopy are not sufficient to gain structural insight into these. A wealth of biochemical and⁄or biophysical data can, however, readily be obtained for biomolecular complexes.

20p awards 05-04-2013 46 3   Download