Microbiological tests for organisms

In view of large number of infections caused by biofilm forming organism, a reliable method for its detection is necessary. Highly accurate methods like Polymerase Chain Reaction (PCR) are available for detection of biofilm, but these are beyond the scope of majority of microbiology laboratories in developing nations like India.

5p nguathienthan8 20-10-2020 10 1   Download

The nanocapsules were prepared by the interfacial deposition technique proposed in the year 1989. The required quantity of the drug and benzyl benzoate were mixed and added dropwise in the organic phase containing the polymer PLGA dissolved in the two organic solvents. With continuous stirring at 4000rpm for 1 hour, soyalecithin was added in the above mixture. Finally, the later was added in the solution of Polaxamer 188 with a continuous stirring speed of 4000rpm for 30 minutes.

8p angicungduoc6 22-07-2020 6 1   Download

Breast milk is the best source of nutrition as it is composed of all bio active components required for the growth and development of infant. Even after the advances in infant formula mixes, breast milk is universally accepted to be best suited to infants. The objective of this study is to ensure quality of donated breast milk as a safe end product. The study is done to determine the prevalence of potentially pathogenic micro-organism that indicates hygiene and sanitary conditions of human milk collected at milk bank.

4p chauchaungayxua6 26-06-2020 9 0   Download

India accounts for 30% of neonatal deaths globally. Bacterial sepsis is a significant cause of morbidity and mortality in newborns. The study helps to make antibiotic policy in neonatal sepsis. The main objective is to study the incidence of multidrug resistant gram negative and gram positive organisms causing neonatal septicemia and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology over a period of one year. Sample of blood was collected under aseptic precautions and processed by standard techniques.

5p gaocaolon5 14-06-2020 20 2   Download

Nowadays the technology has rapidly advanced in the detection of mycobacterium tuberculosis and drug susceptibility clinical sample. The examination that include the advances microscopic, in viro-culture and application in molecular base. In developing countries the world health organization is played a role for evaluating and developing the technology of their efficiency, feasibility at present in India, natural network and designed microbiology centre, and also intermediate reference laboratories implemented by the revised national tuberculosis control programme RNTCP.

9p nguaconbaynhay5 11-05-2020 8 0   Download

The accumulation of Cr in soil is a major concern because of its possible phytotoxicity or increased movement of metals into food chain and the potential for surface and ground water contamination. Excessive metal accumulation in contaminated soil can result in decrease in soil microbiological activities, soil fertility and overall soil quality which may lead to reduction in yield.

13p caygaocaolon4 04-04-2020 14 2   Download

The present study was conducted with the aim to record seroprevalence and microbiological studies in serum and milk samples and vaginal swabs respectively, of brucellosis in goats. A total of 110 serum samples, 13 milk samples and 50 vaginal swabs were collected randomly from various organized and unorganized goat farms, regardless of breed and age (1year-9 years) of goats. Out of total 110 serum samples examined, 06 (05.45%) and 04 (03.63%) samples were found to be positive by Rose Bengal Plate Test and Standard Tube Agglutination Test, respectively. Out of 13 milk samples 04 (30.

9p caygaocaolon2 11-03-2020 15 1   Download

Infection complicates the pathological picture of diabetic foot and plays a main role in development of moist gangrene. A total sample size of 100 patients with diabetic foot ulcer was included in this cross sectional study. A detailed history was obtained and assessment of clinical symptoms and signs were made. Discharge from the lesions, pus aspirates from the abscesses and debrided necrotic materials were collected. Aerobic culture anerobic culture and antibiotic susceptibility testing was done. Males were the predominant group in this study 66% as compared to females 34%.

5p cothumenhmong3 22-02-2020 26 0   Download

Chronic Suppurative Otitis Media (CSOM) is a well known disease for its persistence and recurrence in spite of treatment. It was found to be the single major cause of conductive deafness, speech disorders. It involves considerable morbidity and can cause extra and intracranial complications. Due to development of beta lactamase producing microorganisms, drug resistant strains emerge making drug resistance a major cause of treatment failure. The isolation of Methicillin Resistant Staphylococcus aureus (MRSA) was reported within 1year of introduction of methicillin.

9p trinhthamhodang3 22-02-2020 25 0   Download

Marbofloxacin is a third generation quinolone developed exclusively for veterinary use. The disposition kinetic profile of marbofloxacin was generated after a single intravenous bolus injection in male Sahiwal calves (n = 5) at the dose level of 2 mg.kg-1 body weight. Marbofloxacin concentrations in plasma were determined by microbiological assay using Escherichia coli MTCC 443 as the test organism. The plasma concentration–time profile following intravenous administration was best described by a two-compartment open model. The plasma drug concentration ≥ 0.12 μg.ml-1 was detected at 24 h.

8p chauchaungayxua3 07-02-2020 13 1   Download

he aim of this study was to assess the toxicity of dispersants (OSD/SEACARE and OSD/LT) with different concentrations on the growth of Aspergillus and Rhizopus species in fresh water (Ocdee River) in KegbaraDereGokana Local Government Area, Rivers State. The Fresh water sample was collected by submerging 10liters of jerry Can (sterile) into a depth of 30cm in K-Dere River in Gokana Local Government Area, Rivers State. The samples were transported to the laboratory in sterile containers packed in coolers in an ice pack for analyses within six hours of collection.

10p nguaconbaynhay3 07-02-2020 9 0   Download

NFGNB also known as Non-fermenters (NFs) are emerging with increasing frequency as agents of opportunistic and often, serious infection as well as nosocomial infection. NFGNB are innately resistant to many antibiotics and are known to produce extended spectrum β-lactamases and metallo β-lactamases (MBL).Very few laboratories in India identify these organisms as routine as they are slow growing and requires special media and biochemical tests for identification. The rates of isolation of NFGNBs are increasing in Clinical Microbiology Laboratory, hence this study was done.

10p nguaconbaynhay3 07-02-2020 24 1   Download

Bacterial infections are most commonly encountered by clinicians in developing countries. Area-specific monitoring studies done to assess the type of pathogens and their antibiotic sensitivity patterns which help the clinician to opt for best empirical treatment. The hospital based cross sectional study is conducted on various samples collected using sterile techniques from 2474 clinically-suspected cases attending RDBP, Jaipuriya Hospital from Jan 2016 to Dec 2017 and tested bacteriologically using standard conventional procedures.

9p chauchaungayxua3 07-02-2020 24 1   Download

Over the period of years infections caused by multidrug resistance organisms has emerged as a major public health problem. Their prevalence rates vary in different parts of the world and hence local data regarding these pathogens were important. Our study was aimed to identify the presence of ESBL and AmpC producing Enterobacteriaceae isolates from various clinical samples in our hospital setup various clinical samples were processed consecutively during the study period for microbiological analysis as per standard operating procedure.

9p quenchua2 16-12-2019 21 1   Download

Ear infections usually results from bacterial, fungal, viral infections. Ear infections are major health problem and occur with a high incidence and prevalence in both developed and developing countries which leads to deafness and intra-cranial complications. It may be acute, chronic or recurrent. It can be suppurative or non-suppurative. The Aim of study is to determine Bacterial and Mycological profile and their antimicrobial sensitivity pattern of Ear Infections. Total 95 patients of Ear Infections are included in this study.

8p kethamoi2 15-12-2019 24 1   Download

Very few therapeutic options are available from the time of emergence of MRSA. Macrolide lincosamide streptogramin B (MSLB) antibiotics can be used in such scenarios with clindamycin being the preferred agent due to its excellent pharmacokinetic properties. Staphylococcal resistance to clindamycin may be inducible (iMLSB - inducible MacrolideLincosamide Streptogramin B resistance) or constitutive. The treatment of patients harbouring iMLSB Staphylococci with clindamycin leads to the development of constitutive resistance, subsequently leading to therapeutic failure.

8p kequaidan2 13-12-2019 32 0   Download

Pseudomonas aeruginosa are established pathogens predominantly in various nosocomial infections which are often life threatening due to limited therapeutic options. The present study was undertaken to analyze the antibiotic susceptibility pattern in the clinical isolates of Pseudomonas aeruginosa, so as to establish the current therapeutic options available for treatment in this geographical area of North East India. The study was conducted on 150 strains of Pseudomonas aeruginosa isolated from various clinical samples.

8p kequaidan2 11-12-2019 13 0   Download

Staphylococcus haemolyticus is part of the human normal skin microflora and is also found in nonhuman primates. This organism has been documented as a cause of primary and nosocomial bacteremia in clinical health care settings. Though CONS is ruled out as a contaminant, the emergence of multidrug-resistant isolates to glycopeptides, methicillin, fluoroquinolones, macrolides, lincosamides, streptogramin B and linezolid results in treatment failure in primary bacteremia.

8p kequaidan2 11-12-2019 14 0   Download

Among medical care equipments Stethoscope is one of the most frequently used device. Stethoscopes are mainly used by the health professionals for the assessment of the patients and have been reported to be one of the common vectors for the nosocomial infection in various parts of the world. The contamination of Stethoscope particularly the diaphragm is mainly reported due to the lack of regular disinfection.

7p nguaconbaynhay1 04-12-2019 15 1   Download

Cefquinome is fourth generation cephalosporin developed exclusively for veterinary use. The present research was aimed to investigate the disposition kinetic profile of cefquinome at the dose level of 2 mg.kg-1 body weight following single intramuscular administration. Cefquinome concentrations in plasma were determined by microbiological assay technique using Kocuria rhizophila MTCC 1541 as the test organism. The plasma concentration– time profile following intramuscular administration was best described by onecompartment open model. The peak plasma concentration (Cmax) of 5.79 ± 0.35 μg.

7p chauchaungayxua1 04-12-2019 9 1   Download