Molecular genetic methods

Understanding the genetic complexity of traits is an important objective of small grain temperate cereals yield and adaptation improvements. Bi-parental quantitative trait loci (QTL) linkage mapping is a powerful method to identify genetic regions that co-segregate in the trait of interest within the research population. However, recently, association or linkage disequilibrium (LD) mapping using a genome-wide association study (GWAS) became an approach for unraveling the molecular genetic basis underlying the natural phenotypic variation.

17p partimesinhvien 08-05-2020 17 1   Download

Research objectives: Thesis was the first studied methodically systematic assessment of genetic diversity by morphological indicators combined with molecular markers, identification of Vietnamese native Dendroium species based on the ITS sequences. The results of the thesis have significance in the classification, the conservation service, and selection and breed new varieties; these will be contributing to improve Vietnam orchids.

27p change03 06-05-2016 74 6   Download

Research in diatom biology has entered the postgenomic era since the recent completion of the Thalassiosira pseudonanagenome project. How-ever, the molecular tools available for genetic manipulation of diatoms are still sparse, impeding the functional analysis of diatom genes in vivo. Here we describe the first method for inducible gene expression in transgenic diatoms.

11p fptmusic 11-04-2013 45 2   Download

In view of the increasing number of reported concentration oscillations in living cells, methods are needed that can identify the causes of these oscillations. These causes always derive from the influences that concentrations have on reaction rates. The influences reach over many molecular reaction steps and are defined by the detailed molecular topology of the network. So-called
autoinfluence paths, which quantify the influence of one molecular species upon itself through a particular path through the network, can have positive or negative values....

11p awards 05-04-2013 46 5   Download

Weak and transient protein–protein interactions are associated with biolog-ical processes, but many are still undefined because of the difficulties in their identification. Here, we describe a redesigned method to screen transient protein–protein interactions by using a novel signal amplification circuit, which is incorporated into yeast to artificially magnify the signal responding to the interactions.

9p cosis54 04-01-2013 53 4   Download

The cationic peptide antibiotic polymyxin has recently been reevaluated in the treatment of severe infections caused by gram negative bacteria. Methods: In this study, the genetic determinants for capsular polysaccharide level and lipopolysaccharide modification involved in polymyxin B resistance of the opportunistic pathogen Klebsiella pneumoniae were characterized. The expressional control of the genes responsible for the resistance was assessed by a LacZ reporter system.

16p toshiba23 18-11-2011 48 1   Download