Molecular genetic methods

Lung cancer is the most frequent cause of cancer deaths in both men and women in the U.S. (1). Although tobacco smoking is accepted as the number one cause of this devastating disease, our understanding of the acquired genetic changes leading to lung cancer is still rudimentary. Lung cancer is classifi ed into two major clinic-pathological groups, small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC) (2). Squamous cell carcinoma, adenocarcinoma, and large cell carcinoma are the major histologic types of NSCLC....

531p tom_123 15-11-2012 40 7   Download

Biotechnology is the scientific field of studying and applying the most efficient methods and techniques to get useful end-products for the human society by using viable micro-organisms, cells, and tissues of plants or animals, or even certain functional components of their organisms, that are grown in fully controlled conditions to maximize their specific metabolism inside fully automatic bioreactors.

288p ktct_1669 09-05-2012 181 51   Download

It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies.

469p codoc_pn87 24-11-2012 66 22   Download

During the last three decades, important advances have been made in the available treatments for the loss of skeletal tissue as a result of trauma or disease. The application of large skeletal allografts and total joint replacement have become successful and reproducible treatment options. Unfortunately there still is a significant incidence of failure because of mechanical or biological complications.

0p chuyenphimbuon 21-07-2012 39 14   Download

1 Molecular Genetics in Ecology What is Molecular Ecology? Over the past 20 years, molecular biology has revolutionized ecological research. During that time, methods for genetically characterizing individuals, populations and species have become almost routine, and have provided us with a wealth

30p thegun16 29-11-2010 51 6   Download

An ultimate goal of modern biology is to understand how the genetic blueprint of cells (genotype) determines the structure, function, and behavior of a living organism (phenotype). At the center of this scientific endeavor is characterizing the biochemical and cellular roles of proteins, the working molecules of the machinery of life. A key to understanding of functional proteins is the knowledge of their folded structures in a cell, as the structures provide the basis for studying proteins’ functions and functional mechanisms at the molecular level....

407p chuyenphimbuon 21-07-2012 14 4   Download

A suite of emerging techniques, known collectively as “molecular imaging,” now offer scientists an unprecedented opportunity to identify, follow, and quantify biologic processes at the cellular level with molecular specificity in intact organisms. For instance, it is now possible to evaluate, with imaging, the distribution, magnitude, and timing of gene expression in genetically altered animals (1–3).

508p gian_anh 18-10-2012 86 4   Download

The molecular definition of tumor antigens, costimulatory signals, and the possibility to genetically engineer tumor cells as well as simple protocols for efficient isolation and preparation of dendritic cells (DC) renew the interest in tumor immunotherapy and vaccination, in particular. Engineering of tumor cells with the gene of a particular cytokine is a way of releasing that cytokine at the tumor site. In contrast to bolus administration, it provides a constant supply of cytokine.

634p tom_123 15-11-2012 42 3   Download

Research objectives: Thesis was the first studied methodically systematic assessment of genetic diversity by morphological indicators combined with molecular markers, identification of Vietnamese native Dendroium species based on the ITS sequences. The results of the thesis have significance in the classification, the conservation service, and selection and breed new varieties; these will be contributing to improve Vietnam orchids.

27p change03 06-05-2016 26 2   Download

In chickpea breeding genetic studies of individual plants need to be evaluated at the DNA level using molecular markers. A simple and reliable DNA extraction method is a prerequisite. This small-scale method is cetyltrimethylammonium bromide (CTAB)-based and extracts DNA from 1 to 3 folded young leaves processed in a 1.5 ml tube with 0.5 ml of extraction buffer and homogenized using an electric drill.

5p vitamix 15-02-2019 4 1   Download

Despite advances in pharmacology, not all patients respond favourably to drugs. A proportion of patients under therapy don't benefit from their treatment or experience an adverse reaction to the medication. However, progress in the understanding of disease mechanisms and drug actions are opening opportunities to match therapies to patient populations, and thus pave the way towards a more personalised medicine.

0p quynho77 13-11-2012 22 0   Download

Turkey, the third leading apple producer in world, produces approximately 2.6 million tons of apples per year. Venturia inaequalis, the causal agent of apple scab, is a major fungal disease among apples varieties. This study was conducted in accordance with the plant protection method for isolates collected from the apple production areas in Isparta Province.

10p danhdanh11 09-01-2019 4 0   Download

An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated.

16p sony2711 28-01-2019 7 0   Download

Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants.

10p sony2711 01-02-2019 4 0   Download

Plant response to reduced water availability and other abiotic stress (e.g. metals) have been analysed through changes in water absorption and transport mechanisms and also by molecular and genetic approach. A relatively new aspects of fruit nutrition are presented in order to provide the basis for the improvement of some fruit quality traits. The involvement of hormones, nutritional and proteomic plant profiles together with some structure/function of sexual components have also been addressed.

398p conquynho32 14-09-2012 78 29   Download

The clinical and basic science of allergic disease is moving and evolving so rapidly that it is nearly impossible to keep up full of information regarding the news mechanisms, signalling cell markers, cellular plasticity and expression, biological mediators targets or molecular and genetic discovers aspects related to the allergy. Moreover the systemic impact of this pathology determines the complexity of the more recent evidence demonstrates that the rapid regulation of central immune organs in clinical response of the mucosa to the allergen or offending agent.

284p wqwqwqwqwq 21-07-2012 79 12   Download

Lung cancer is the leading cause of cancer death among men and women in the United States with 170,000 deaths per year. This exceeds the sum of the next three leading causes of death due to cancer: breast, colon, and prostate. There are over 1 million deaths worldwide due to lung cancer, making it truly an epidemic. Fewer than 15% achieve a 5-yr survival. The vast majority (85%) present with advanced disease, although stage I patients may have a 5-yr survival approaching 70% (1).

721p tom_123 15-11-2012 38 9   Download

Twenty-five years ago, Georges Köhler and César Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics (1). However, in spite of their early promise, monoclonal antibodies (MAbs) were largely unsuccessful as therapeutic reagents resulting from insufficient activation of human effector functions and immune reactions against proteins of murine origin.

456p tom_123 15-11-2012 54 7   Download

I would like to express my deepest gratitude to all theauthors who contributed to this book by sharing their valuable works with us. This book should prove useful to students, researchers, and experts in the area of conservation biology, genetic diversity, and molecular biology. The year 2010 was celebrated as the international year of biodiversity by the United Nations and it has been a unique opportunity to realize the vital role that biodiversity plays in sustaining the life on Earth.

284p conquynho32 18-09-2012 48 6   Download

There is huge uncertainty about future budget deficits and their financing. Economists disagree about how quickly deficits should be reduced: some would stress deflation risks and others inflation risks. Even if economists were to agree, there would still be great uncertainty about political choices on macroeconomic policy. It is nevertheless certain that government debt/GDP ratios in major countries will continue to rise over the next few years. Even the optimistic G20 pronouncements do not envisage debt/GDP ratios in the advanced countries stabilising before 2016.

97p taisaovanchuavo 23-01-2013 34 6   Download