Molecular identification

Master's thesis of Science "Investigation into the modulation of circadian clock proteins by dietary compounds and small molecules" is structured as follows: Chapter 1 Introduction; Chapter 2 Identification of novel bioactive compounds from Olea europaea by evaluation of chemical compounds in the OliveNetTM library: in silico bioactivity and molecular modelling, and in vitro validation of hERG activity; Chapter 3 Modulation of circadian core clock proteins by dietary compounds; Chapter 4 Molecular mechanisms of action of selected olive phenolics against epigenetic modifiers and the structur...

235p runthenight04 02-02-2023 5 3   Download

Campylobacter species are a leading cause of food-borne disease and C. jejuni highlight the most potential public health impact of Campylobacter contamination by pathogens originating from animals or animal products.. The total of 33meat samples comprising 8 from sheep (5) and goat (3) also 25 beef samples were screened by morphological, biochemical and molecular technique.

9p chauchaungayxua11 23-03-2021 17 2   Download

Evaluate genetic diversity of Lien Minh chickens based on sequences of mitochondrial DNA D-loop region; determine the genetic relationship and the origin of Lien Minh chickens; identif genetic relationships between candidate genes and egg yield traits in Lien Minh chickens.

27p kequaidan7 01-09-2020 32 3   Download

The present study was conducted to study the prevalence of the food borne zoonotic pathogen of animal origin, L. monocytogenes by isolation and identification, molecular detection and antibiotic sensitivity pattern from different samples of cattle sources in Aizawl district of Mizoram. A total 200 numbers of sample including cattle faeces (50), raw milk (50) and milk products (100) were collected randomly from different unorganized shop and farms.

15p gaocaolon5 14-06-2020 12 2   Download

Understanding the genetic complexity of traits is an important objective of small grain temperate cereals yield and adaptation improvements. Bi-parental quantitative trait loci (QTL) linkage mapping is a powerful method to identify genetic regions that co-segregate in the trait of interest within the research population. However, recently, association or linkage disequilibrium (LD) mapping using a genome-wide association study (GWAS) became an approach for unraveling the molecular genetic basis underlying the natural phenotypic variation.

17p partimesinhvien 08-05-2020 17 1   Download

Research objectives: Thesis was the first studied methodically systematic assessment of genetic diversity by morphological indicators combined with molecular markers, identification of Vietnamese native Dendroium species based on the ITS sequences. The results of the thesis have significance in the classification, the conservation service, and selection and breed new varieties; these will be contributing to improve Vietnam orchids.

27p change03 06-05-2016 74 6   Download

Diagnostic tests by molecular biology is made for studying the relations among Fusarium species for linking production of proteins, degree of relationship and occurrence of malformation. Determination of proteins for isolates causing-disease by SDS-PAGE explained there’s specific band for each fungus and there are common bands among some isolates of fungi. Since, band with MW 30 KDa represented only in F. proliferatum and F. oxysporum and F. subgluti-nans respectively. This band considered as specific band for these isolates, which released high patho-genisity effect.

7p zingzing09 24-04-2013 58 3   Download

Glucose transporters (GLUTs) have been implicated in adaptive and survival responses to hypoxic stress in mam-mals. In fish, the expression and regulation ofGLUT in relation to hypoxia remains unexplored. Here we describe the identification ofa hypoxia-responsive glucose transpor-ter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans
11 kb ofgenomic sequence and consists of12 exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a poly-peptide of533 amino acids, with a predictedmolecularmass of
57 kDa and a pI of8.34....

8p fptmusic 16-04-2013 40 3   Download

As a first step toward developing a zebrafish model for investigating the role of sulfation in counteracting environ-mental estrogenic chemicals, we have embarked on the identification and characterization of cytosolic sulfotrans-ferases (STs) in zebrafish. By searching the zebrafish expressed sequence tag database, we have identified two cDNA clones encoding putative cytosolic STs. These two zebrafish ST cDNAs were isolated and subjected to nuc-leotide sequencing. Sequence data revealed that the two zebrafishSTs are highlyhomologous, being
82%identical in their amino acid sequences....

8p fptmusic 16-04-2013 41 2   Download

Numerous adhesion molecules have been described, and the molecular mechanisms of lymphocyte trafficking across the endothelium is starting to be elucidated. Identification of the molecules involved in the organoselec-tivity of this process would help in the targeting of drug therapy to specific tissues. Adhesion-regulating molecule-1 (ARM-1) is an adhesion-regulating molecule previously identified on T cells. It does not belong to any known families of adhesion molecules.

12p awards 06-04-2013 35 4   Download

We report here the identification of a tachykinin-related peptide (TRP) in crustaceans. Direct MALDI-TOF MS withbrainslicesof the crayfishProcambarus clarkiiindicated the presence of a peptide having an MS number of around 934. Quadrupole orthogonal acceleration time-of-flight (Q-TOF) MS/MS analysis implied the sequence to be APSGFLGMRamide, identical to that of CabTRP Ia, isolated previously from the crab Cancer borealis,and Pev-tachykinin, from the shrimpPenaeus vannamei.

11p dell39 03-04-2013 36 2   Download

Reliable detection and identification of pathogens in complex biological samples, in the presence of contaminating DNA from a variety of sources, is an important and challenging diagnostic problem for the development of field tests. The problem is compounded by the difficulty of finding a single, unique genomic sequence that is present simultaneously in all genomes of a species of closely related pathogens and absent in the genomes of the host or the organisms that contribute to the sample background. ...

11p dell39 27-03-2013 35 4   Download

Anserinase (Xaa-methyl-His dipeptidase, EC 3.4.13.5) is a dipeptidase that mainly catalyzes the hydrolysis ofNa-acetylhistidine in the brain, retina and vitreous body of all poikilothermic vertebrates. The gene encoding anserinase has not been previously identified. We report the molecular identification of anserinase, purified from brain of Nile tilapia Oreochromis niloticus.

13p dell39 27-03-2013 42 5   Download

The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history.

12p viettel02 22-02-2013 37 3   Download

The isovaleric acid-emanating silkworm mutantskunk (sku) was first stud-ied over 30 years ago because of its unusual odour and prepupal lethality. Here, we report the identification and characterization of the gene responsi-ble for theskumutant.

12p viettel02 19-02-2013 31 2   Download

Peptidomics is a powerful set of tools for the identification, structural eluci-dation and discovery of novel regulatory peptides and for monitoring the degradation pathways of structurally and catalytically important proteins. Amphibian skin secretions, arising from specialized granular glands, often contain complex peptidomes containing many components of entirely novel structure and unique site-substituted analogues of known peptide families.

13p mobifone23 18-01-2013 48 3   Download

In the early 1990s, breakthrough discoveries on the genetics of Alzheimer’s disease led to the identification of missense mutations in the amyloid-b precursor proteingene. Research findings quickly followed, giving insights into molecular pathogenesis and possibilities for the development of new types of animal models.

21p mobifone23 18-01-2013 41 5   Download

In the present study, we report the identification of a new gene from the Bacillus subtilisB3 strain (aatB3), which comprises 1308 bp encoding a 436 amino acid protein with a monomer molecular weight of 49.1 kDa. Phylo-genetic analyses suggested that this enzyme is a member of the Ib subgroup of aspartate aminotransferases

13p cosis54 05-01-2013 51 4   Download

Weak and transient protein–protein interactions are associated with biolog-ical processes, but many are still undefined because of the difficulties in their identification. Here, we describe a redesigned method to screen transient protein–protein interactions by using a novel signal amplification circuit, which is incorporated into yeast to artificially magnify the signal responding to the interactions.

9p cosis54 04-01-2013 53 4   Download

Phage display technology is a powerful selection approach to identify strong and specific binders to a large variety of targets. In this study, we compared the efficacy of a phage library displaying human heavy chain complementarity determining region 3 (HCDR3) repertoires with a set of conventional random peptide libraries for the identification of CXCR4

12p cosis54 04-01-2013 45 3   Download