Molecular tools

In this paper a molecular dynamics simulation of nano-metric cutting of copper with a diamond tool is presented. MD simulations require the determination of the interaction of the involved atoms through a function of potential for the materials involved in the analysis and the accurate topography of the studied area, leading to high demand of computational time.

12p tohitohi 19-05-2020 27 1   Download

Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools.

17p vishikamaru2711 29-04-2020 19 3   Download

Objects of research: Appraising of the species diversity and the variation according to space of Chrysomelidae in Nui Chua national park. Determining the food of Chrysomelidae and appraising the change of their food according to space.

25p xacxuoc4321 09-07-2019 35 8   Download

Chapter 5 - Molecular tools for studying genes and gene activity. This chapter describe the most popular techniques that molecular biologists use to investigate the structure and function of genes. Most of these start with cloned genes. Many use gel electrophoresis. Many also use labeled tracers, and many rely on nucleic acid hybridization. We have already examined gene cloning techniques.

82p tangtuy05 30-03-2016 37 2   Download

Oceans provide enormous and diverse space for marine life. Invertebrates are conspicuous inhabitants in certain zones such as the intertidal; many are soft- bodied, relatively immobile and lack obvious physical defenses. These animals frequently have evolved chemical defenses against predators and overgrowth by fouling organisms. Marine animals may accumulate and use a variety of toxins from prey organisms and from symbiotic microorganisms for their own purposes. Thus, toxic animals are particularly abundant in the oceans.

23p uocvong01 24-09-2015 63 7   Download

The decoy approach against nuclear factor kB (NF-kB) is a useful tool to alter NF-kB dependent gene expression using synthetic oligonucleotides (ODNs) carrying NF-kB specific cis-elements. Unfortunately, ODNs are not stable and need to be extensively modified to be used in vivo or ex vivo. We have previously evaluated the possible use of peptide nucleic acids (PNAs) as decoy molecules. The backbone of PNAs is composed of N-(2-aminoethyl)glycine units, rendering these molecules resistant to both nucleases and proteases.

10p system191 01-06-2013 42 4   Download

The envelope protein gp64 of the baculovirusAutographa californicanuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein bothmediates pH-dependentmembrane fusionandbinds tohost cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology.

10p research12 23-04-2013 27 2   Download

Multi-protein complexes are emerging as important entities of biological activity inside cells that serve to create func-tional diversity by contextual combination of gene products and, at the same time, organize the large number of different proteins into functional units. Many a time, when studying protein complexes rather than individual proteins, the bio-logical insight gained has been fundamental, particularly in cases in which proteins with no previous functional anno-tation couldbe placed intoa functional context derived from their
molecular environment. ...

9p tumor12 20-04-2013 37 2   Download

Research in diatom biology has entered the postgenomic era since the recent completion of the Thalassiosira pseudonanagenome project. How-ever, the molecular tools available for genetic manipulation of diatoms are still sparse, impeding the functional analysis of diatom genes in vivo. Here we describe the first method for inducible gene expression in transgenic diatoms.

11p fptmusic 11-04-2013 44 1   Download

In view of the increasing number of reported concentration oscillations in living cells, methods are needed that can identify the causes of these oscillations. These causes always derive from the influences that concentrations have on reaction rates. The influences reach over many molecular reaction steps and are defined by the detailed molecular topology of the network. So-called
autoinfluence paths, which quantify the influence of one molecular species upon itself through a particular path through the network, can have positive or negative values....

11p awards 05-04-2013 46 5   Download

DNA helicases are molecular
motor enzymes that use the energy of NTP hydrolysis to separate transiently energetic-ally stable duplex DNA into single strands. They are there-fore essential in nearly all DNA metabolic transactions. They act as essential molecular tools for the cellular machinery. Since the discovery of the first DNA helicase in Escherichia coliin1976, several havebeen isolated fromboth prokaryotic and eukaryotic systems.

15p dell39 03-04-2013 50 4   Download

Amylosucrase is a transglycosidase which belongs to family 13 of the glyco-side hydrolases and transglycosidases, and catalyses the formation of amy-lose from sucrose. Its potential use as an industrial tool for the synthesis or modification of polysaccharides is hampered by its low catalytic efficiency on sucrose alone, its low stability and the catalysis of side reactions result-ing in sucrose isomer formation.

9p dell39 27-03-2013 25 3   Download

The formation of amyloid fibrils is associated with various human medical disorders of unrelated origin. Recent research indicates that self-assembled amyloid fibrils are also involved in physiological processes in several micro-organisms. Yet, the molecular basis for the recognition and self-assembly processes mediating the formation of such structures from their soluble protein precursors is not fully understood.

8p dell39 27-03-2013 38 5   Download

The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history.

12p viettel02 22-02-2013 36 3   Download

High-throughput metabolomics is a dynamically developing technology that enables the mass separation of complex mixtures at very high resolu-tion. Metabolic profiling has begun to be widely used in clinical research to study the molecular mechanisms of complex cell disorders.

11p viettel02 22-02-2013 33 4   Download

Phage display of antibody libraries is a powerful tool for antibody discov-ery and evolution. Recombinant antibodies have been displayed on phage particles as scFvs or Fabs, and more recently as bivalent F(ab¢)2.We recently developed a technology (E-clonal) for screening of combinatorial IgG libraries using bacterial periplasmic display and selection by fluores-cence-activated cell sorting (FACS) [Mazor Yet al.

13p mobifone23 21-01-2013 48 4   Download

Fluorescent proteins have become essential tools in molecular and biologi-cal applications. Here, we present a novel fluorescent protein isolated from warm water coral, Cyphastrea microphthalma. The protein, which we named vivid Verde fluorescent protein (VFP), matures readily at 37C and emits bright green light.

12p mobifone23 21-01-2013 53 4   Download

Peptidomics is a powerful set of tools for the identification, structural eluci-dation and discovery of novel regulatory peptides and for monitoring the degradation pathways of structurally and catalytically important proteins. Amphibian skin secretions, arising from specialized granular glands, often contain complex peptidomes containing many components of entirely novel structure and unique site-substituted analogues of known peptide families.

13p mobifone23 18-01-2013 48 3   Download

Cells have evolved complex and overlapping mechanisms to protect their proteins from aggregation. However, several reasons can cause the failure of such defences, among them mutations, stress conditions and high rates of protein synthesis, all common consequences of heterologous protein pro-duction.

11p cosis54 05-01-2013 53 4   Download

Proteinase inhibitors (PIs) are widely distributed in plants, microorganisms and mammalian species; they are known to play pivotal roles in regulating proteinase activities in these species. Accordingly, PIs are important molecular tools for many research disciplines. Studies of the correlation of PI structures and functions have lead to understanding of their inhibitory actions against physiological important proteases. There are potential therapeutic applications for such studies for diseases relating to excessive activities of proteases.

8p tuanloc_muido 11-12-2012 43 5   Download