Monooxygenases

Ngày nay, các công nghệ sản xuất các hóa chất có giá trị thƣơng mại từ các chất không tan trong nƣớc nhƣ các hydrocarbon từ nguồn dầu mỏ bằng xúc tác sinh học đang rất đƣợc quan tâm. Các tế bào thƣờng đƣợc sử dụng làm xúc tác sinh học vì có sẵn các cofactor (NADH,…) và có khả năng tái tạo các cofactor này. Khả năng tái tạo các cofactor của tế bào rất cần thiết cho các phản ứng oxy hóa và khử. Trong ngành công nghệ hóa chất, nguyên liệu để tổng hợp hóa chất thƣờng...

41p canhchuon_1 21-06-2013 90 13   Download

This paper describes the cloning of the genes coding for each component of the complex of toluene/o-xylene monooxy-genase fromPseudomonas stutzeriOX1, their expression, purification and characterization. Moreover, the reconsti-tution of the active complex from the recombinant subunits has been obtained, and the functional role of each compo-nent in the electron transfer from the electron donor to molecular oxygen has been determined.

11p tumor12 22-04-2013 44 3   Download

The catalytic activity of phenylalanine hydroxylase (PAH, phenylalanine 4-monooxygenase EC 1.14.16.1) is regulated by three main mechanisms, i.e. substrate (L-phenylalanine, L-Phe) activation, pterin cofactor inhibition and phos-phorylation of a single serine (Ser16) residue.To address the molecular basis for the inhibition by the natural cofactor (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin, its effects on the recombinant tetrameric human enzyme (wt-hPAH) was studied using three different conformational probes, i.e.

10p tumor12 20-04-2013 34 3   Download

Soluble methane monooxygenase (sMMO) is a three-com-ponent enzyme that catalyses dioxygen- and NAD(P)H-dependent oxygenation of methane and numerous other substrates. Oxygenation occurs at the binuclear iron active centre in the hydroxylase component (MMOH), to which electrons are passed from NAD(P)H via the reductase component (MMOR), along a pathway that is facilitated and controlledby the third component, proteinB (MMOB).

6p tumor12 20-04-2013 34 3   Download

Alkene monooxygenase (AMO) fromRhodococcus rhodochrous(formerly Nocardia corallina) B-276 belongs to a family of multicomponent nonheme binuclear iron-centre oxygenases that includes the soluble methane mono-oxygenases (sMMOs) found in some methane-oxidizing bacteria. The enzymes catalyse the insertion of oxygen into organic substrates (mostly hydrocarbons) at the expense of O2and NAD(P)H.

0p awards 06-04-2013 26 2   Download

Planarians are one of the simplest animal groups with a central nervous system. Their primitive central nervous system produces large quantities of a variety of neuropeptides, of which many are amidated at their C terminus. In vertebrates, peptide amidation is catalyzed by two enzymes [peptidylglycine a-hydroxylating monooxygenase (PHM) and peptidyl-a-hydroxylglycinea-amidating lyase] acting sequentially. In mammals, both enzymatic activities are contained within a single protein that is encoded by a single gene....

14p awards 05-04-2013 42 4   Download

The Baeyer–Villiger monooxygenase (BVMO), 4-hydroxy-acetophenone monooxygenase (HAPMO), uses NADPH andO2 to oxidize a variety of aromatic ketones and sulfides. The FAD-containing enzyme has a 700-fold preference for NADPH over NADH. Sequence alignment with other BVMOs, which are all known to be selective for NADPH, revealed three conservedbasic residues,whichcouldaccount for the observed coenzyme specificity. The corresponding residues in HAPMO (Arg339, Lys439 and Arg440) were mutated and the properties of the purified mutant enzymes were studied. ...

10p dell39 03-04-2013 28 2   Download

Vitamin C, a reducing agent and antioxidant, is a cofactor in reactions cata-lyzed by Cu + -dependent monooxygenases and Fe 2+ -dependent dioxygenas-es. It is synthesized, in vertebrates having this capacity, fromd-glucuronate. The latter is formed through direct hydrolysis of uridine diphosphate (UDP)-glucuronate by enzyme(s) bound to the endoplasmic reticulum mem-brane, sharing many properties with, and most likely identical to, UDP-glucuronosyltransferases.

22p inspiron33 23-03-2013 53 5   Download

Cytochrome P450 oxidase (thường viết tắt là CYP) là một thuật ngữ chung chỉ một nhóm enzyme ôxi hóa khử (EC 1.4) có liên quan, nhưng khác biệt nhau, quan trọng trong sinh lý động vật có xương sống. Hệ thống Cytochrome P450 monooxygenase hỗn hợp chức năng hầu như là yếu tố quan trọng nhất của Pha I biến dưỡng ở lớp thú.

2p teutomeo 08-09-2011 137 21   Download