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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex
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This paper describes the cloning of the genes coding for each component of the complex of toluene/o-xylene monooxy-genase fromPseudomonas stutzeriOX1, their expression, purification and characterization. Moreover, the reconsti-tution of the active complex from the recombinant subunits has been obtained, and the functional role of each compo-nent in the electron transfer from the electron donor to molecular oxygen has been determined.
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