![](images/graphics/blank.gif)
Mouse cells and embryos
-
Ebook "p53 Protocols (Second edition)" expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.
251p
dongmelo
27-05-2024
3
2
Download
-
Part 1 book "Transgenic mouse - Methods and protocols" includes content: Introduction - Strategies for developing genetically modified mice; genetic modification of the mouse - general technology – pronuclear and blastocyst injection; generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos; cryopreservation of mouse spermatozoa and in vitro fertilization; autopsy and histological analysis of the transgenic mouse; transgene design; inducible transgenic mouse models; lentiviral transgenesis; transgenesis in mouse embryonic stem cells.
175p
oursky09
12-11-2023
4
3
Download
-
Part 1 book "RT-PCR Protocols" includes content: Single cell RT-PCR on mouse embryos - A general approach for developmental biology; detection of influenza a virus neuraminidase and pb2 gene segments by one step reverse transcription polymerase chain reaction; RT-PCR amplification and cloning of large viral sequences,... and other contens.
189p
oursky05
20-09-2023
1
0
Download
-
Part 1 book "Transgenic mouse, methods and protocols" includes content: Introduction - Atrategies for developing genetically modified mice; genetic modification of the mouse - general technology – pronuclear and blastocyst injection; generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos; cryopreservation of mouse spermatozoa and in vitro fertilization,.... and other contents.
175p
oursky05
20-09-2023
3
1
Download
-
Part 1 book "Avian embryology" includes content: Operations on primitive streak stage avian embryos; quail–chick transplantations, other chimeras - quail–duck and mouse–chick; manipulations of neural crest cells or their migratory pathways; embryo slices and strips - guidance and adhesion assays in the avian embryo; embryo slices and strips - guidance and adhesion assays in the avian embryo; cell division, diverentiation, and death in avian embryos;.... and other contents.
227p
oursky05
20-09-2023
2
2
Download
-
In this research, we evaluated the preimplantation development, embryo morphology, the time of cell division and the cell number of the blastocyst stage of single mouse blastomeres biopsied from 8-cell embryos. One blastomere was biopsied from each 8-cell embryo using micromanipulation system with XYClone laser.
8p
vimalfoy
08-02-2023
1
1
Download
-
This study examined the effects of Scriptaid, a histone deacetylase inhibitor (HDAC) during the first mitotic cell cycle on preimplantation development of mouse embryos generated by injection of round spermatid into mature oocytes.
8p
vimalfoy
08-02-2023
4
1
Download
-
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing.
11p
viarchimedes
26-01-2022
8
0
Download
-
In this study, three alkaloid fractions from Huperzia serrata (Thunb.) Trevis, Lycopodium clavatum L. and Huperzia squarrosa (G. Forst.) Trevis were tested in two toxicity models: mouse primary neuronal cell (in vitro) and zebrafish (Danio rerio) embryos (in vivo).
9p
guitaracoustic02
08-12-2021
15
1
Download
-
Long non-coding RNAs (lncRNAs), a type of epigenetic regulator, are thought to play important roles in embryonic development in mice, and several developmental defects are associated with epigenetic modification disorders.
13p
vitzuyu2711
29-09-2021
19
1
Download
-
Genetic studies in mouse have demonstrated the crucial function of PAX4 in pancreatic cell differentiation. This transcription factor specifies β- and δ-cell fate at the expense of α-cell identity by repressing Arx gene expression and ectopic expression of PAX4 in α-cells is sufficient to convert them into β-cells.
16p
vimariana2711
22-12-2020
7
0
Download
-
Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox17 and Cxcr4 are useful markers of the DE. Previously, we identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos.
9p
viwyoming2711
16-12-2020
14
1
Download
-
C-lectin family 14 Member A (Clec14a) is a transmembrane protein specifically expressed in vascular endothelial cells during embryogenesis. Previous in vitro and in vivo studies have provided conflicting data regarding Clec14a role in promoting or inhibiting angiogenesis, therefore its functional role in vascular development remains poorly understood.
10p
viwyoming2711
16-12-2020
9
2
Download
-
Mammalian early embryo development requires a well-orchestrated interplay of cell signaling pathways. Notch is a major regulatory pathway involved in cell-fate determination in embryonic and adult scenarios. However, the role of Notch in embryonic pre-implantation development is controversial.
16p
viwyoming2711
16-12-2020
13
1
Download
-
Analysis of single cells in their native environment is a powerful method to address key questions in developmental systems biology. Confocal microscopy imaging of intact tissues, followed by automatic image segmentation, provides a means to conduct cytometric studies while at the same time preserving crucial information about the spatial organization of the tissue and morphological features of the cells.
19p
vioklahoma2711
19-11-2020
16
2
Download
-
The in vitro antimicrobial potency of the bacteriocin AS-48 is well documented, but its clinical application requires investigation, as its toxicity could be different in in vitro (haemolytic and antibacterial activity in blood and cytotoxicity towards normal human cell lines) and in vivo (e.g. mice and zebrafish embryos) models. Overall, the results obtained are promising.
11p
trinhthamhodang1
14-11-2019
18
1
Download
-
We have previously reported a mouseRnf33⁄Trim60gene that is temporally expressed in the pre-implantation embryo. TheRnf33structural gene is com-posed of a short noncoding exon 1 and an intronless coding exon 2. In the present work,Rnf33was shown to be expressed in the mouse testis and in the testicular cell lines TM3 and TM4.
14p
mobifone23
08-01-2013
48
2
Download
-
Human embryonic stem cell (hESC) lines, like those from other species, are pluripotent cell lines derived from the inner cell mass (ICM) of the developing embryo. Due to their exceptional capability of proliferating indefi nitely as undifferentiated cells when cultured in appropriate conditions, and of sustaining a normal karyotype, hESCs may have broad applications for industrial uses; clinical purposes, namely, cell-based therapy; and research of early human development, differentiation mechanisms, and lineage commitment.
157p
thienbinh1311
13-12-2012
50
7
Download
CHỦ ĐỀ BẠN MUỐN TÌM
![](images/graphics/blank.gif)