mRNA degradation
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Ebook "RNA exosome" is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of this volume to introduce this fascinating protein complex as well as to give a timely and rich account of its many functions.
161p cotieubac1004 15-03-2024 1 0 Download
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MicroRNAs (miRNAs) are a novel class of endogenous, non-coding, single-stranded RNAs capable of regulating gene expression by suppressing translation or degrading mRNAs. Single nucleotide polymorphisms (SNP) can alter miRNA expression, resulting in diverse functional consequences.
10p vinarcissa 21-03-2023 3 1 Download
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Hypoxia inducible factor-1α (HIF-1) and HIF-1 degrading prolyl-hydroxylases (PHD) are key regulators of the hypoxic response but are also involved in many cellular actions. Furthermore, we could recently show that leukocyte HIF-1α-mRNA-expression is decreased in severe sepsis and inversely correlated with disease severity.
9p viottohahn 30-03-2022 14 2 Download
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Cells have evolved quality control mechanisms to ensure protein homeostasis by detecting and degrading aberrant mRNAs and proteins. A common source of aberrant mRNAs is premature polyadenylation, which can result in non-functional protein products.
20p vielonmusk 30-01-2022 10 0 Download
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Alu elements are major contributors to lineage-specific new exons in primate and human genomes. Recent studies indicate that some Alu exons have high transcript inclusion levels or tissue-specific splicing profiles, and may play important regulatory roles in modulating mRNA degradation or translational efficiency. However, the contribution of Alu exons to the human proteome remains unclear and controversial.
14p viaristotle 29-01-2022 10 0 Download
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Gene expression is controlled at multiple levels, including transcription, stability, translation, and degradation. Over the years, it has become apparent that Plasmodium falciparum exerts limited transcriptional control of gene expression, while at least part of Plasmodium’s genome is controlled by post-transcriptional mechanisms.
18p viaristotle 29-01-2022 10 0 Download
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Nonsense-mediated mRNA decay (NMD) is a eukaryotic, translationdependent degradation pathway that targets mRNAs with premature termination codons and also regulates the expression of some mRNAs that encode full-length proteins.
23p viarchimedes 26-01-2022 16 0 Download
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Yaks that inhabit the Tibetan Plateau exhibit striking phenotypic and physiological differences from cattle and have adapted well to the extreme conditions on the plateau. However, the mechanisms used by these animals for the regulation of gene expression at high altitude are not fully understood.
13p vilarryellison 29-10-2021 6 1 Download
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In this study, we evaluated the performance of 3′ mRNA-Seq using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD with UMI, comparing with TruSeq Stranded mRNA-Seq and RNA Exome Capture kit. The fresh-frozen (FF) and FFPE tissues yielded nucleotide sizes range from 13 to > 70% of DV200 values; input amounts ranged from 1 ng to 100 ng for validation.
9p vilarryellison 29-10-2021 10 1 Download
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The main bottleneck for genomic studies of tumors is the limited availability of fresh frozen (FF) samples collected from patients, coupled with comprehensive long-term clinical follow-up. This shortage could be alleviated by using existing large archives of routinely obtained and stored Formalin-Fixed Paraffin-Embedded (FFPE) tissues. However, since these samples are partially degraded, their RNA sequencing is technically challenging.
11p vibeauty 23-10-2021 14 0 Download
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The genome-wide mRNA half-lives were determined by a dynamic analysis of transcriptomes after transcription arrest. We have combined the analysis of mRNA half-lives with the steady-state concentrations (transcriptome) to provide an integrated overview of the in vivo activity of these exoribonucleases at the genome-scale.
13p vitzuyu2711 29-09-2021 6 1 Download
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Anastomosis group 1 IA (AG1-IA) of Rhizoctonia solani is the major agent of banded leaf and sheath blight (BLSB) disease that causes severe yield loss in many worldwide crops. MicroRNAs (miRNAs) are ~ 22 nt noncoding RNAs that negatively regulate gene expression levels by mRNA degradation or translation inhibition.
18p vijichea2711 28-05-2021 11 1 Download
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MicroRNAs (miRNAs) are short (19-23 nucleotides) non-coding RNAs that bind to sites in the 3’untranslated regions (3’UTR) of a targeted messenger RNA (mRNA). Binding leads to degradation of the transcript or blocked translation resulting in decreased expression of the targeted gene.
10p vikentucky2711 26-11-2020 8 0 Download
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The regulation of all gene expression steps (e.g., Transcription, RNA processing, Translation, and mRNA Degradation) is known to be primarily encoded in different parts of genes and in genomic regions in proximity to genes (e.g., promoters, untranslated regions, coding regions, introns, etc.).
10p vioklahoma2711 19-11-2020 14 1 Download
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MicroRNAs carry out post-transcriptional gene regulation in animals by binding to the 3' untranslated regions of mRNAs, causing their degradation or translational repression. MicroRNAs influence many biological functions, and dysregulation can therefore disrupt development or even cause death.
13p viflorida2711 30-10-2020 15 2 Download
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Analysis of high-throughput multi-’omics interactions across the hierarchy of expression has wide interest in making inferences with regard to biological function and biomarker discovery. Expression levels across different scales are determined by robust synthesis, regulation and degradation processes, and hence transcript (mRNA) measurements made by microarray/RNA-Seq only show modest correlation with corresponding protein levels.
13p vicolorado2711 23-10-2020 9 1 Download
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Previously we have suggested that cancer cells develop a mechanism(s) which allows for either: Silencing of the wild-type TP53 transcription, degradation of the wild-type TP53 mRNA, or selective overproduction of the mutated TP53 mRNA, which is the subject of this article.
11p vilisa271 22-09-2020 11 0 Download
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ZFP36 is an mRNA binding protein that exerts anti-tumor activity in glioblastoma by triggering cell death, associated to an increase in the stability of the kinase RIP1. Methods: We used cell death assays, size exclusion chromatography, Co-Immunoprecipitation, shRNA lentivectors and glioma neural stem cells to determine the effects of ZFP36 on the assembly of a death complex containing RIP1 and on the induction of necroptosis.
7p vialabama2711 21-09-2020 9 1 Download
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MicroRNAs (miRNAs) are highly conserved class of short endogenous non-coding small RNA molecules of about 18–22 nucleotide in length. MicroRNAs negatively regulate the gene expression by degrading target mRNA. In the present study, comparative genomic based approach was used to identify and characterize new conserved microRNAs in “orphan legume crop” pigeon pea (Cajanus cajan L.) using expressed sequence tags (ESTs) by in silico method. A total of 4,621 unique previously reported microRNAs were used for homology search in 25,576 ESTs of pigeon pea for identifying conserved miRNAs.
11p nguaconbaynhay6 24-06-2020 12 2 Download
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MicroRNAs (miRNAs) are non-coding RNA molecules which have recently emerged as important gene regulators in plants and their gene expression analysis is becoming increasingly important. miRNAs regulate gene expression at the post-transcriptional level by translational repression or target degradation of specific mRNAs and gene silencing.
19p viminato2711 22-05-2020 9 1 Download