MS medium

In this study, the effect of plant growth regulators (BA and NAA), ripe bananas in combination with potato, humic acid, and silver nanoparticles (AgNPs) were investigated to find a suitable condition for in vitro plant regeneration from R. imschootiana protocorm-like body (PLB). The results showed that after 45 days of culture, MS medium supplemented with 100 g ripe banana/liter and 100 g potato/liter was suitable for regenerating shoots from the protocorm-like body (PLB) (17.56 shoots/PLBs and 100.00% of shoot-regenerating PLBs).

13p dianmotminh02 03-05-2024 4 1   Download

This study investigated the effects of different PGRs on in vitro plant regeneration of Anthurium scherzerianum Schott and acclimatization in the ex vitro stage. Depending on the objective of each experiment, the in vitro shoots were induced from callus cultured on a modified Murashige and Skoog (MS) medium containing cytokinins and auxins.

11p vicaptainmarvel 21-04-2023 3 2   Download

In this study the effectiveness of plant growth regulators on micropropagation, total phenol content and metabolic profiles of Melissa officinalis L., important medicinal herb was assessed. The stem segments derived from one-month old in vitro germinated seedlings were used for initial explants. Of the eight different nutrient media tested the most favorable for micropropagation were found to be MP2 (MS medium enriched with 1mg/L BAP and 0.1 mg/L IBA) and MP3 (MS supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA), which yielded 3.25- 4.0 shoots per explant within 4 weeks of culture.

5p mudbound 10-12-2021 16 1   Download

An efficient regeneration protocol was established for a rare plant species, Begonia coptidifolia. Using leaf explants, adventitious shoots were induced on Murashige and Skoog (MS) basal medium using various concentrations (1.0–10.0 mM) of three cytokinins, 6-benzyladenine (BA), thidiazuron (TDZ) and kinetin (KIN). However, adventitious shoots could not be induced on MS medium supplemented with auxins [α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)] tested in the 1.0–10.0 mM range.

8p hanthienngao 30-11-2021 11 1   Download

Water availability is a very important factor for the growth and development of plants, which limits the plant production capacity. Rootstocks are widely utilized to improve plants tolerance to various biotic and abiotic stresses. In this study, physiological and biochemical responses of fifteen almond rootstock candidates to drought stress were investigated under in vitro conditions. The shoot tips from fifteen almond rootstock candidates were cultured in MS medium containing 1.0 mg/L BAP, 0.01 mg/L IBA, 30 g/L sucrose and 7 g/L agar.

11p hanthienngao 30-11-2021 14 1   Download

The cotyledons are suitable materials to create multiple shoot for GmCHI transgenic T. paniculatum when using the MS medium supplemented with kinetin. The addition of 2.0 mg/l of BAP gave a higher multi-shooting effect than the addition of 1.5 mg/l of kinetin or the combination of 1.5 mg/l of kinetin with 0.8 mg/l of IBA. Using 0.5mg/l IAA had better rooting ability than combining 0.5mg/l IAA with kinetin.

8p princessmononoke 28-11-2021 9 1   Download

The obtained results show that FeNPs added to MS medium was higher growth, chlorophyll (a, b and a+b) content, antioxidant activity of SOD and APX enzymesthan Fe-EDTA in MS medium as control treatment. The effect of FeNPs are differences between culture systems. In vitro solid and microponic culture systems, the optimal concentration is 75 mM FeNPs and in vitro hydroponic culture system is 100 mM FeNPs.

13p spiritedaway36 25-11-2021 3 1   Download

Roses are one of the most popular fl owers in the world because of their beauty, color, aroma as well as their various uses and Red Eden rose is one of the most popular hybrid roses. Results of the study showed the most optimal medium for shoot multiplication, which was MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 0.5 mg/l of BA. The optimal medium for growth and fl owering was ½MS + 30 g/l of sucrose + 8 g/l of agar supplemented with 30 μM of AgNO3 . However, NAA at all tested concentrations did not show any eff ect on in vitro plant growth.

7p nguaconbaynhay12 03-06-2021 23 2   Download

Production of bamboo plants by using vegetative propagation is not simple to establish a commercial fields however micropropagation techniques has much ability to achieve the bamboo seedling demands. In vitro cultures of Bambusa balcooa were achieved by using nodal segment as an explant. Eight different combinations of plant growth regulators with MS basal medium were used for shoot induction and multiplication.

7p trinhthamhodang11 27-04-2021 15 2   Download

The micropropagation protocol of tropical, small evergreen, true mangrove, and woody shrub or tree species, Aegiceras corniculatum, has been standardized. Axillary shoot proliferation was induced in vitro from nodal explants excised from 3-4 years field-grown old plant which was then used as explants for the establishment of in vitro cultures. Surface-sterilized explants were cultured on the Murashige and Skoog (MS) basal medium supplemented with different concentrations and a combination of growth regulators.

7p caygaocaolon11 21-04-2021 4 1   Download

The present research was focused in standardizing the callus induction technique and studies the behaviour of callus in secondary metabolite production in Andrographis echioides (L.) Nees. In this study, the leaf bits of Andrographis echioides responded positively for callus induction. Earliest (21.00 days) and profuse callusing was observed on MS medium supplemented with 2, 4-D (2.0 mgl-1 ) + NAA (2.0 mgl-1 ) + BAP (2.0 mgl-1 ).

8p chauchaungayxua10 19-03-2021 11 2   Download

The basic MS containing 20g/l sucrose, 8g/l agar, TDZ 4μM and NAA 1μM was the most optimum medium for shoot propagation from seedlings and gave the best shoot quality. NAA had little effect on generating roots of Dendrobium chrysotoxum in vitro.

7p trinhthamhodang1218 18-03-2021 8 2   Download

In this study, the removal of matrix and isobaric interferences for the quantitative determination of 87 Sr/86 Sr isotopic ratio using ICP-MS was focused. The matrix effect caused by calcium at high concentration in petroleum drill-holes water samples was eliminated by using anion exchanger (BioRad AG1x8) in HNO3-methanol (95%) medium, meanwhile the isobaric effect due to rubidium presented in sample was removed by cation resin Bio-Rad AG50x8.

10p trinhthamhodang1218 14-03-2021 13 1   Download

The present investigation was carried out to study the influence of antioxidants on in vitro culture of Gliricidia sepium. Soft nodal stem segments were inoculated on MS medium supplemented with desired concentrations plant growth regulator such as cytokinins (BAP/Kinetin) and different antioxidants. Antioxidants viz., activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation.

6p trinhthamhodang1218 26-02-2021 11 1   Download

In addition, the net photosynthetic rate (Pn) of plants under PA condition was statistically higher and increased over the culture period while Pn of plants under PM condition remained constant. This study demonstrated that MS salts with half concentration of NH4NO3 and photoautotrophic culture method were appropriate for in vitro growth of hydrangea.

11p nguaconbaynhay10 20-02-2021 11 2   Download

An experiment on callus induction and indirect regeneration of strawberry cv. Chandler was conducted by taking different explants (internodal cuttings, matured leaves, young leaves, flower buds) were cultured on MS medium supplemented with different growth regulators and incubated in culture room for callus induction. Expansion and swelling of explants at the cut surface were noted and soft crystal, greenish compact calli were emerged from the cut surface within 3-4 weeks culture on MS medium supplemented with 2, 4- D (1.0 mg/l) along with BAP (0.1 mg/l).

8p cothumenhmong9 18-01-2021 8 2   Download

In this study, we investigated four factors to improve the hairy root induction in Urena lobata L. These factors include: age of plant (15-day-old in vitro plants, 45-day-old in vitro plants and after two subculture generations plants), different parts of plant (roots, stems, and leaves), infection time (10, 20 and 30 minutes), and culture medium (Murashige and Skoog (MS), Gamborg B5 medium (GB5) and Woody plant medium (WPM)).

8p larachdumlanat127 02-01-2021 5 2   Download

The present experiment was conducted to standardize the protocol for in vitro mutagenesis of gladiolus hybridus varieties ‘Punjab Glance’ and ‘Sylvia’. The nodal buds were irradiated with different doses of gamma rays (Co60) 0, 5, 8, 10 and 12 Gy. Irradiated explants were then cultured aseptically on MS basal medium supplemented with 5 mg/l (BAP) + 2mg/l (IBA) and agar-agar to induce shoot and root proliferation.

11p gaocaolon9 22-12-2020 9 3   Download

The Multi-stripe Travelling Salesman Problem (Ms-TSP) is an extension of the Travelling Salesman Problem (TSP). In the q-stripe TSP with q ≥ 1, the objective function sums the costs for traveling from one vertex to each of the next q vertices along the tour. To solve medium to large-sized instances, a metaheuristic approach is proposed. The proposed method has two main components, which are construction and improvement phases. The construction phase generates an initial solution using the Greedy Randomized Adaptive Search Procedure (GRASP).

18p nguathienthan9 08-12-2020 13 2   Download

Different nutrient solutions alone or in combinations were applied as foliar spray on Asiatic lilium hybrid Tresor and evaluated for different characters. Plants sprayed with only water were taken as control. Other treatments were done with the dose of nutrients used in preparation of tissue culture Murashige and Skoog (MS) medium (1962) in different combinations.

7p caygaocaolon8 07-11-2020 15 2   Download