Multiplex PCR method

Ebook "How to reliably test for GMOs" have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed.

110p nhanphanguyet 28-01-2024 5 2   Download

Ebook "T-cell differentiation: Methods and protocols" provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails,...

272p lucchinguyen 28-12-2023 6 2   Download

Part 1 book "Protocols for the diagnosis of pig viral diseases" includes content: Requirements and preparedness for attending a viral disease outbreak in pig farms; collection of samples, their preservation and transportation; methods for quantification of viruses; protocols for isolation of genetic materials from RNA viruses; multiplex pcr for diagnosis of porcine diseases; protocols for isolation of plasmid DNA,... and other contents.

203p muasambanhan02 18-12-2023 6 3   Download

Part 1 book "ELISA - Methods and protocols" includes content: Flow through assay for detection of antibodies using protein a colloidal gold conjugate as a probe; multiplexed microsphere suspension array based immunoassays; lateral flow immunoassay, immuno pcr assay for sensitive detection of proteins in real time; tyramide signal amplification for immunofluorescent enhancement;.... and other contents.

116p oursky05 20-09-2023 2 2   Download

Group B streptococcus (GBS) infections are still the leading cause of invasive infections in neonates, specically they also seriously cause mortality and morbidity with underlying diseases in adults. Curently, there are ten GBS serotypes (Ia, Ib, and II-IX) and the resistance characteristic of GBS is important to clinical treatment.

5p vicaptainmarvel 21-04-2023 8 2   Download

The incidence of hypervirulent epidemic Clostridium difficile has increased around the world and now in Australia. Assays that are capable of rapidly identifying these strains would enable earlier diagnosis and timely infection control response. The aims of the first part of the study were to validate and develop a molecular technique for the rapid diagnosis of toxigenic C.

134p runthenight07 01-03-2023 6 2   Download

This study was aimed to investigate the intestinal microbiota in racing pigeons with regard to Enterococcus species distribution, virulence factors and antibiotic susceptibility. Three methods (API, Multiplex sodA-PCR, 16S rRNA sequencing) were compared for Enterococcus species identification. Cloacal samples from 179 apparently healthy pigeons of 13 different flocks were tested.

14p vigalileogalilei 28-02-2022 22 1   Download

We describe a highly sensitive, quantitative, and inexpensive technique for targeted sequencing of transcript cohorts or genomic regions from thousands of bulk samples or single cells in parallel. Multiplexing is based on a simple method that produces extensive matrices of diverse DNA barcodes attached to invariant primer sets, which are all pre-selected and optimized in silico.

16p vielonmusk 30-01-2022 21 0   Download

This study was conducted to detect Ascaris suum, Toxocara canis, and Toxocara cati in naturally infected chicken livers, and then to provide information related to ascarid infections in humans. Ninetyfour chicken liver samples collected at a fresh market were used for this study. DNA was extracted from each minced liver sample (500 mg) by the alkaline lysis method using NaOH 50mM and Tris-HCl (pH 8.0).

6p chauchaungayxua12 09-07-2021 12 2   Download

Culturing of bronchoalveolar lavage (BAL) fluid is a commonly used method for pathogen detection in pneumonia. However, the sensitivity is low, especially in patients pre-treated with anti-infective agents. The early detection of a pathogen is crucial for the outcome of respiratory tract infections.

8p vimaine2711 26-03-2021 9 2   Download

The present study was undertaken with an objective of phenotypic and molecular detection of beta-lactam antimicrobial resistance in E. coli isolated from freshwater fish in Andhra Pradesh.

7p gaocaolon9 22-12-2020 11 2   Download

In this study, 100 specimens from tuberculosis confirmed patients and suspected patients who were treated in Hai Duong Tuberculosis and Lung Disease Hospital to perform 3 simultaneous M. tuberculosis detection methods as multiplex PCR assay, direct sputum smear (AFB) and bacterial culture (BK).

7p tamynhan9 02-12-2020 13 2   Download

Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more efficient than both the target enrichment method and Sanger sequencing.

9p viflorida2711 30-10-2020 15 1   Download

Standard RNAseq methods using bulk RNA and recent single-cell RNAseq methods use DNA barcodes to identify samples and cells, and the barcoded cDNAs are pooled into a library pool before high throughput sequencing. In cases of single-cell and low-input RNAseq methods, the library is further amplified by PCR after the pooling.

9p vicolorado2711 23-10-2020 11 0   Download

Persistent high-risk human papillomavirus (HPV) infection is correlated with an increased risk of developing intraepithelial lesion or malignancy (NILM). The aims of the current study is to establish a method named BioPerfectus Multiplex Real Time (BMRT) HPV assay for simultaneous typing and quantifying HPVs, and to evaluate it by comparison with HPV GenoArray test and PCR-sequencing method, as well as histological status.

11p vimoscow2711 29-08-2020 19 0   Download

Interrogate the impact of IKZF1 deletion on therapy-outcomes of adults with common B-cell acute lymphoblastic leukemia. Methods: One hundred sixty-five consecutive adults with common B-cell ALL were tested for IKZF1 deletion and for BCR/ABL. Deletions in IKZF1 were detected using multiplex RQ-PCR, multiplex fluorescent PCR, sequence analysis and multiplex ligation-dependent probe amplification (MLPA).

8p vinaypyidaw2711 26-08-2020 11 1   Download

Food authenticity is currently an issue of major concern for food authorities, since incorrect labelling of animal foods may have remarkable negative consequences. To circumvent this problem, DNA based method had been utilised. The present study was carried out for the detection of meat species by using variability in ATPase Subunit 6 and 8 genes by multiplex PCR.

7p nguaconbaynhay7 15-08-2020 14 2   Download

Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences.

12p vihamax2711 21-04-2020 7 0   Download

Amoebiasis, caused by Entamoeba histolytica, remains a serious public health issue worldwide. For decades, microscopy remains preferred method for detection of the pathogenic species. The recent advent of molecular biology has helped in developing appropriate diagnostic technologies, of which isothermal amplification is considered to be robust and cost-effective compared to PCR that requires expensive instruments and highly skilled personnel.

6p quenchua4 06-04-2020 11 1   Download

E. coli O157:H7 is an emerging food borne pathogen having zoonotic significance. Though the primary reservoir of this serotype is cattle; Sheep and goat are also considered to be the main reservoir for E. coli O157:H7. Conventional cultural methods are time consuming method and also the specificity is very less. Multiplex PCR (mPCR) tends to be specific, more rapid and reliable.

9p chauchaungayxua4 18-03-2020 17 0   Download