Mutant generation

Ebook "p53 Protocols (Second edition)" expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.

251p dongmelo 27-05-2024 3 2   Download

Ebook "Hematopoietic stem cell development" brings together articles covering the biology of hematopoietic stem cells during embryonic development, reporting particular aspects of fly, fish, avian and mammalian models. Thus, this book allows a comparative overview of hematopoietic stem cell generation in the different classes, emphasizing the conserved trends in development. This book culminates with state-of –the art knowledge on hematopoietic development in human species and discussions of the most recent breakthroughs with relevance to both researchers and clinicians.

188p ladongphongthanh1008 22-04-2024 5 2   Download

Ebook "Transgenic microalgae as green cell factories (Advances in experimental medicine and biology, Volume 616)" book includes chapters focussing on biotechnological applications of transgenic microalgae. The enormous amount information generated by the genomic or cDNA sequencing projects and its implications on the development of new biotechnological applications of microalgae are analysed in Chapter 6.

145p duongthandue0501 28-02-2024 7 1   Download

Ebook "Ion channels (Topics in medicinal chemistry, Volume 3)" play a vital role in basic physiological functions such as generation of electrical activity in nerves and muscle, control of cardiac excitability, intracellular signaling, hormone secretion, cell proliferation and many other biological processes. Because of their prevalence and the critical role they play in virtually all tissue types and organs, ion channels are also involved in a number of pathophysiological conditions. The aim of this volume is to review recent advances in the field of ion channel related diseases.

177p manmanthanhla0201 27-02-2024 7 1   Download

Part 2 book "Transgenic mouse - Methods and protocols" includes content: Introduction - Engineering BAC reporter gene constructs for mouse transgenesis; targeting vector construction through recombineering; generating conditional knockout mice; hypomorphic mice; micer targeting vectors for manipulating the mouse genome; knock in approaches; generation of a series of knock in alleles using rmce in es cells; selection of targeted mutants from a library of randomly mutagenized ES cells.... and other contents.

190p oursky09 12-11-2023 7 2   Download

In the paper, a black knifefish-inspired robot with an elongated undulating fin is investigated for locomotion control using an improved CPG model. The proposed CPG network has sixteen fin rays connected Hopf oscillators for creating fish-like swimming gaits. To identify a set of optimal parameters for the modified CPG network, mutant particle swarm optimization (MPSO), an improved form of particle swarm optimization (PSO), is introduced.

9p giangnhanly 02-10-2023 12 5   Download

Part 2 book "Transgenic mouse, methods and protocols" includes content: Engineering BAC reporter gene constructs for mouse transgenesis; targeting vector construction through recombineering, generating conditional knockout mice, hypomorphic mice, knock in approaches, selection of targeted mutants from a library of randomly mutagenized ES cells,...and other contents.

190p oursky05 20-09-2023 1 1   Download

Part 2 book "Vertebrate embryogenesis - Embryological, cellular, and genetic methods" includes content: Assisted reproductive technology in nonhuman primates, ciona genetics, reptile embryology, mutant generation in vertebrate model organisms by tilling, laboratory guidelines for animal care, keeping two animal systems in one lab – a frog plus fish case study

293p oursky05 20-09-2023 4 1   Download

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) provide a better prognosis in EGFR-mutant non-small cell lung cancer (NSCLC). Nevertheless, the outcome of leptomeningeal metastasis (LM) remains poor. In addition, due to limited access to intracranial tumour tissue, gene alterations associated with leptomeningeal metastasis from lung adenocarcinoma (LM-LUAD) are unclear.

8p visusanwojcicki 28-06-2022 5 2   Download

Osimertinib—the third-generation epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI)—has been widely used as an first-line treatment for patients with metastatic EGFR-mutant non-small cell lung cancer (NSCLC).

13p visusanwojcicki 28-06-2022 10 2   Download

Long intergenic RNAs (lincRNAs) play critical roles in eukaryotic cells, but systematic analyses of the lincRNAs of an animal for phenotypes are lacking. We generate CRISPR knockout strains for Caenorhabditis elegans lincRNAs and evaluate their phenotypes.

19p vigalileogalilei 27-02-2022 7 1   Download

A new study finds that splicing disruption is a frequent consequence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can express aberrant proteins. This new information allows enhanced quality control procedures to select the best mutant alleles generated by CRISPR/Cas9.

4p vialfrednobel 29-01-2022 14 0   Download

Drought is a major threat to wheat production worldwide and development of drought-tolerant wheat genotypes may improve the production of this cereal crop in drought affected regions. In the present study, 24 mutant lines were developed by treating a wheat variety ‘NN-Gandum-1(NN-1) with ethyl methane sulfonate (EMS). The M5 , M6 , and M7 generations were evaluated for quantitative traits under well irrigated and rainfed conditions. Furthermore, best performing mutants of M6 and M7 generations were examined for biochemical parameters.

17p hanthienngao 30-11-2021 16 1   Download

The colonies expressed the highest cellulose hydrolysis capacity with maximum HC value were obtained at dose range of 700-1500 Gy. It is important to notice the 5 potential mutants including 3 mutants of Aspergillus (TTG-700, TTG-1000 and TTG-1200) and 2 mutants of Trichoderma (VTCC-1000, VTCC-1500) demonstrated the higher CMCase secretion (1.78 – 2.48 times) compared to the wild types. After 5 generations.

8p spiritedaway36 25-11-2021 10 1   Download

Photorhabdus luminescens is an enteric bacterium, which lives in mutualistic association with soil nematodes and is highly pathogenic for a broad spectrum of insects. A complete genome sequence for the type strain P. luminescens subsp. laumondii TT01, which was originally isolated in Trinidad and Tobago, has been described earlier. Subsequently, a rifampicin resistant P. luminescens strain has been generated with superior possibilities for experimental characterization.

17p vitzuyu2711 29-09-2021 8 1   Download

Plasmodium falciparum exhibits resistance to the artemisinin component of the frontline antimalarial treatment Artemisinin-based Combination Therapy in South East Asia. Millions of lives will be at risk if artemisinin resistance (ART-R) spreads to Africa. Single non-synonymous mutations in the propeller region of PF3D7_1343700,“K13” are implicated in resistance. In this work, we use transcriptional profiling to characterize a laboratory-generated k13 insertional mutant previously demonstrated to have increased sensitivity to artemisinins to explore the functional role of k13.

14p vitzuyu2711 29-09-2021 9 1   Download

The effective use of mutant populations for reverse genetic screens relies on the population-wide characterization of the induced mutations. Genome- and population-wide characterization of the mutations found in fast neutron populations has been hindered, however, by the wide range of mutations generated and the lack of affordable technologies to detect DNA sequence changes.

16p visilicon2711 20-08-2021 8 1   Download

Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of various DNA regulatory elements and their mutant variants. The assays are based on construction of highly diverse plasmid libraries containing two variable fragments, a region of interest (a sequence under study; ROI) and a barcode (BC) used to uniquely tag each ROI, which are separated by a constant spacer sequence.

10p visilicon2711 20-08-2021 9 1   Download

Integration of T-DNA into plant genomes via Agrobacterium may interrupt gene structure and generate numerous mutants. The T-DNA caused mutants are valuable materials for understanding T-DNA integration model in plant research.

11p visilicon2711 20-08-2021 7 1   Download

New breeding technologies (NBT) using CRISPR/Cas9-induced homology directed repair (HDR) has the potential to expedite genetic improvement in aquaculture. The long generation time in Atlantic salmon makes breeding an unattractive solution to obtain homozygous mutants and improving the rates of perfect HDR in founder (F0) fish is thus required.

8p vijeeni2711 24-07-2021 12 0   Download