Mycobacterium smegmatis

A series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration and paper chromatography and TLC. Their structures were determined by HPLC and GLC to determine sugar composition and ratios, MALDI-TOF MS to measure molecular mass, methylation analysis to determine linkages, 1H-NMR to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and anomeric configurations of the monosaccharides....

8p system191 01-06-2013 36 5   Download

Trehalose synthase (TreS) catalyzes the reversible inter-conversion of trehalose (glucosyl-a,a-1,1-glucose) and maltose (glucosyl-a1-4-glucose). TreS was purified from the cytosol ofMycobacterium smegmatisto give a single protein band on SDS gels with a molecular mass of
68 kDa. However, active enzyme exhibited a molecular mass of
390 kDa by gel filtration suggesting that TreS is a hexa-mer of six identical subunits.

11p awards 05-04-2013 56 3   Download

Trehalose is a nonreducing disaccharide of glucose (a,a-1,1-glucosyl-glu-cose) that is essential for growth and survival of mycobacteria. These organisms have three different biosynthetic pathways to produce trehalose, and mutants devoid of all three pathways require exogenous trehalose in the medium in order to grow.

14p galaxyss3 21-03-2013 40 3   Download

CmtR is a repressor of the ArsR–SmtB family fromMycobacterium tuber-culosis that has been shown to sense Cd(II) and Pb(II) in Mycobacte-rium smegmatis. We establish here that CmtR binds cooperatively to multiple sites inM. tuberculosisDNA and protects an unusually long 90 bp AT-rich sequence from )80 bp to +10 with respect to its own initiation codon.

12p viettel02 22-02-2013 33 2   Download

Two structures of monomeric methionyl-tRNA synthetase, fromMycobac-terium smegmatis, in complex with the ligands methionine⁄adenosine and methionine, were analyzed by X-ray crystallography at 2.3 A˚ and at 2.8 A˚ , respectively. The structures demonstrated the flexibility of the multidomain enzyme. A new conformation of the structure was identified in which the connective peptide domain bound more closely to the catalytic domain than described previously.

16p viettel02 19-02-2013 42 3   Download

Mycobacterium tuberculosis, the most successful bacterial pathogen, causes tuberculosis, a disease that still causes more than 2 million deaths per year. ArylamineN-acetyltransferase is an enzyme that is conserved in most Mycobacteriumspp.

9p cosis54 08-12-2012 48 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?

9p thulanh20 10-11-2011 35 2   Download