Native fluorescence
-
Success of cancer prodrugs relying on a foreign gene requires specific delivery of the gene to the cancer, and improvements such as higher level gene transfer and expression. CNOB (3.3 mg/kg) was injected iv in mice implanted with humanized ChrR6 (HChrR6)-expressing 4T1 tumors.
10p vimale2711 25-08-2020 11 2 Download
-
Rice is an important staple food for more than half of the world’s population and is subject to diseases that often place major biological constraints on production. Sheath blight of rice is caused by a soil borne fungus and biological control of the disease is an efficient substitute to replace chemical methods. In the present study thirty native fluorescent Pseudomonads, an efficient biocontrol agent have been isolated and confirmed by cultural, morphological, biochemical characterization and visualization under UV light.
9p angicungduoc6 20-07-2020 11 1 Download
-
An increase in naturally-occurring porphyrins has been described in the blood of subjects bearing different kinds of tumors, including colorectal, and this is probably related to a systemic alteration of heme metabolism induced by tumor cells.
9p vimanila2711 29-06-2020 31 0 Download
-
Ultraviolet-B (UV-B) radiation can affect several aspects ranging from plant growth to metabolic regulation. Maca is a Brassicaceae crop native to the Andes growing in above 3500 m of altitude.
12p vishikamaru2711 25-04-2020 3 0 Download
-
Glycine soja is a halophytic soybean native to saline soil in Yellow River Delta, China. Photosystem I (PSI) performance and the interaction between photosystem II (PSII) and PSI remain unclear in Glycine soja under salt stress. This study aimed to explore salt adaptability in Glycine soja in terms of photosystems coordination.
13p viuchiha2711 21-04-2020 10 1 Download
-
The effects of water stress on some growth and chlorophyll a fluorescence parameters were investigated in two native black poplar (Populus nigra L.) clones (Kocabey and Gazi) and in two hybrid poplar (Populus × euramericana (Dode) Guinier) clones (I-214 and I-45/51) to determine which among the four clones is the best adapted to water-stress conditions.
9p danhdanh11 09-01-2019 16 1 Download
-
The structures and stabilities of recombinant chickenmuscle troponin I (TnI) and T (TnT) were investigated by a com-bination of bis-ANS binding and equilibrium unfolding studies.Unlike most folded proteins, isolated TnI and TnT bind the hydrophobic fluorescent probe bis-ANS, indicating the existence of solvent-exposed hydrophobic domains in their structures.
8p tumor12 22-04-2013 38 2 Download
-
S100A1 is a typical representative of a group of EF-hand calcium-binding proteins known as the S100 family. The protein is composed of twoasub-units, each containing two calcium-binding loops (N and C). At physiologi-cal pH (7.2) and NaCl concentration (100 mm), we determined the microscopic binding constants of calcium to S100A1 by analysing the Ca 2+ -titration curves of Trp90 fluorescence for both the native protein and its Glu32fiGln mutant with an inactive N-loop.
9p awards 06-04-2013 28 2 Download
-
The structural properties of EspB, a virulence factor of theEscherichia coli O157 type III secretion system, were characterized. Far-UV and near-UV CD spectra, recorded between pH 1.0 and pH 7.0, show that the protein assumesa-helical structures and that some tyrosine tertiary contacts may exist. All tyrosine side-chains are exposed to water, as determined by acryl-amide fluorescence quenching spectroscopy.
13p awards 05-04-2013 24 2 Download
-
The information required for successful assembly of an icosahedral virus is encoded in the native conformation of the capsidproteinand in its interactionwith the nucleic acid. Here we investigated how the packing and stability of virus capsids are sensitive to single amino acid substitutions in the coat protein. Tryptophan fluorescence, bis-8-anilinonaph-thalene-1-sulfonate fluorescence, CD and light scattering were employed to measure urea- and pressure-induced effects on MS2 bacteriophage and temperature sensitive mutants. ...
11p dell39 03-04-2013 34 3 Download
-
The conformational conversion of prion protein (PrP) from a native con-formation to the amyloid form is a hallmark of transmissible spongiform encephalopathies. Conversion is usually monitored by fluorescent dyes, which bind generic amyloids and are less suited for living cell imaging.
13p mobifone23 21-01-2013 38 3 Download
-
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule
3p panasonic01 13-12-2011 51 3 Download