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Nucleotide analogs
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RNA levels detected at steady state are the consequence of multiple dynamic processes within the cell. In addition to synthesis and decay, transcripts undergo processing. Metabolic tagging with a nucleotide analog is one way of determining the relative contributions of synthesis, decay and conversion processes globally.
17p
viaristotle
29-01-2022
11
0
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There is increasing concern about the potential for nephrotoxicity in patients with chronic hepatitis B (CHB) treated long-term with nucleotide analogs.
9p
vienzym2711
30-03-2020
10
2
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The thermal unfoldingofmyosinsubfragment 1 (S1) cleaved by trypsin was studied by differential scanning calorimetry. In the absence of nucleotides, trypsin splits the S1 heavy chain into three fragments (25, 50, and 20 kDa). This cleavage has no appreciable influence on the thermal unfolding of S1 examined in the presence of ADP, in the ternary complexes of S1 with ADP and phosphate analogs, such as orthovanadate (Vi) or berylliumfluoride (BeFx), and in the presence of F-actin.
11p
tumor12
22-04-2013
32
2
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Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleo-tide. These analogs have strong binding affinities for most nucleotide-requiring systems. Their bindings are easily detected by absorption and fluorescence changes in the visi-ble region.Recent years have seendramatic developments in the application of the TNP nucleotide analogs as spectro-scopic probes for the study on the nucleotide-interacting properties of various enzymes and proteins including their mutants.
7p
tumor12
20-04-2013
37
2
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Eight adenosine analogs, 3-deaza-adenosine (DZA), 3-deaza-(±)aristeromycin (DZAri), 2¢,3¢-dideoxy-adenosine (ddAdo), 2¢,3¢-dideoxy-3-deaza-adenosine (ddDZA), 2¢,3¢-dideoxy-3-deaza-(±)aristeromycin (ddDZAri), 3-deaza-5¢-(±)noraristeromycin (DZNAri), 3-deaza-neplanocin A (DZNep), and neplanocin A (NepA), were tested as inhibi-tors of human placentaS-adenosylhomocysteine (AdoHcy) hydrolase. The order of potency for the inhibition of human placental AdoHcy hydrolase was: DZNep NepA DZAriDZNAri DZA ddAdo ddDZAddDZAri....
11p
tumor12
20-04-2013
36
3
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UMP-CMP kinase catalyses an important step in the phosphorylationofUTP, CTPanddCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies. The reactivity of human UMP-CMP kinase towards natural substrates and nucleotide analogs was reexamined. The expression of the recombinant enzyme and conditions for stability of the enzyme were improved. Substrate inhibition was observed for UMP and CMP at concentrations higher than 0.2 mM, but not for dCMP.
7p
fptmusic
16-04-2013
35
2
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Co 2+ binding RNA aptamers were chosen as research models to reveal the structural basis underlying the recognition of Co 2+ by RNA, with the application of two distinct methods. Using the nucleotide analog interfer-ence mapping assay, we found strong interference effects after incorpora-tion of the 7-deaza guanosine phosphorotioate analog into the RNA chain at equivalent positions G27 and G28 in aptamer no.
12p
media19
06-03-2013
26
1
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