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Báo cáo khoa học: Fluorescent and colored trinitrophenylated analogs of ATP and GTP

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:7

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Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleo-tide. These analogs have strong binding affinities for most nucleotide-requiring systems. Their bindings are easily detected by absorption and fluorescence changes in the visi-ble region.Recent years have seendramatic developments in the application of the TNP nucleotide analogs as spectro-scopic probes for the study on the nucleotide-interacting properties of various enzymes and proteins including their mutants. This review is intended as a broad overview of currently extensively used applications of the nucleotide analogs in various biological systems....

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Nội dung Text: Báo cáo khoa học: Fluorescent and colored trinitrophenylated analogs of ATP and GTP

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