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Báo cáo khoa học: Biochemical characteristics of C-terminal region of recombinant chitinase from Bacillus licheniformis – implication of necessity for enzyme properties
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The functional and structural significance of the C-terminal region ofBacil-lus licheniformis chitinase was explored using C-terminal truncation muta-genesis. Comparative studies between full-length and truncated mutant molecules included initial rate kinetics, fluorescence and CD spectrometric properties, substrate binding and hydrolysis abilities, thermostability, and thermodenaturation kinetics.
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