Optimal plant regeneration

In the study "Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/Cas9 genome editing of commercial tomato cultivars", we have optimized an Agrobacterium-mediated gene transfer and regeneration system for CRISPR/Cas9 genome editing in two commercial tomato cultivars for the first time. The effect of explant type, genotype, pre-transformation time, Agrobacterium concentration, infection time, and different co-culture periods of bacteria were evaluated to optimize the regeneration and transformation parameters.

14p lyhuyenthu 31-01-2023 8 2   Download

Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz.

9p hanthienngao 30-11-2021 9 1   Download

Success rate in selection of putative transformants is mainly dependent upon the optimum concentration of selective agent. Effect of antibiotic concentrations of hygromycin (10, 20, 30 and 50 mg/L) and paromomycin (10, 20, 30 and 50 mg/L) in callusing and regeneration media on immature embryo-derived calli was evaluated using wheat variety PBW621.

8p chauchaungayxua8 03-10-2020 13 2   Download

Aromatic rice is profitable farming venture and having high consumer demand. The main concerns about aromatic rice are low yield and susceptibility to pests and diseases. A rapid and reproducible genetic transformation protocol was optimized for three aromatic rice varieties using the established plant regeneration protocol.

30p nguaconbaynhay6 23-06-2020 11 1   Download

Gladiolus (Gladiolus hybrida L.) has been found as a potential cut flower cultivated world widely due to its attractive spikes and elegancy. The plant is propagated vegetatively through corms and cormels but more often its cultivation is hindered due to low multiplication rate of its corm and cormels. Gladiolus can grows through underground stems also, but it is more oftenly attacked by soil borne diseases. In vitro propagation techniques, assumes significance, especially for securing rapid multiplication of the novel cultivars using different explants sources and media.

10p angicungduoc5 12-06-2020 8 2   Download

The aim of the present study was to develop a simplified micropropagation protocol for this species, combining phases of in Vitro propagation and rooting and to assess the genetic stability of in Vitro regenerants using ISSR and flow cytometric analysis. The highest number of roots of all tested treatments was produced on MS medium supplemented with zeatin. Ten randomly chosen in Vitro regenerants were subjected to ISSR and flow cytometric analysis. Thus, the micropropagation method optimized here can be used for highly effective production of true-to-type plants of T.Leontopetaloides.

8p bichxuan01643027348 15-08-2019 22 1   Download