Organic acid

In this study, we investigated some conditions affecting the extraction of total carotenoids from R. mucilaginosa using environmentally friendly chemicals: cell disruption conditions with organic acids combined with ultrasound-assistant method, using ethanol and petroleum ether solvents to replace acetone in the process of extracting total carotenoids to obtain a benign product, easy to apply in food.

8p viling 11-10-2024 2 1   Download

The objective of this study was to determine the impact of the extraction conditions on the extraction yield of bioactive compounds from grape pomace using choline chloride and organic acid-based deep eutectic solvents (DES).

9p viling 11-10-2024 1 0   Download

Automated DNA sequencing is a core research tool used by almost every research biochemistry lab. It is used to determine the sequence of DNA, or the genetic code, that serves as the blueprint of life for every organism on Earth.

78p zingzing09 21-10-2012 63 8   Download

Trace element (iron, manganese, cobalt, copper, zinc, selenium, and molybdenum): chemical element that is needed in minute quantities for the proper growth, development, and physiology of the organism. In biochemistry, a trace element is also referred to as a micronutrient.

25p zingzing09 21-10-2012 87 8   Download

Aim of the thesis: Synthesis of several derivatives and tubulysin analogues with the replacement of the amino acid methylpipecolic (Mep) at the N-terminal, replacing N,O-acetyl group by methyl group, and researching the role of tubuphenylalanine (Tup) group at the C-terminal.

26p extraenglish 24-05-2021 18 4   Download

Synthesis of several derivatives and tubulysin analogues with the replacement of the amino acid methylpipecolic (Mep) at the N-terminal, replacing N,O-acetyl group by methyl group, and researching the role of tubuphenylalanine (Tup) group at the C-terminal .

26p capheviahe27 23-02-2021 20 5   Download

The goals of the dissertation: Synthesizing deep eutectic solvents based on choline chloride with the compounds of 2-alkylbenzimidazole/ethylene glycol and determining their structures; using synthetic deep eutectic solvents to enrich and separate Omega-3,6,9 from waste fat in accordance with the facrories’ procedure of processing Vietnamese basa fish for export.

31p capheviahe27 23-02-2021 33 3   Download

The total synthesis of mycophenolic acid (MPA), a potent immunosuppressant, was modified. The obtained mycophenolic acid was suitable for further preparation of new prospective immunosuppressants with improved therapeutic properties.

8p tocectocec 25-05-2020 31 1   Download

Comparative analysis of genome sequence data from mesophilic and hyperthermophilic micro-organisms has revealed a strong bias against specific thermolabile aminoacid residues (i.e. N and Q) in hyperthermophilic proteins. The N þ Q content of class II xylose isomerases (XIs) from mesophiles, moderate thermophiles, and hyperthermophiles was examined.

11p system191 01-06-2013 33 4   Download

Institute for Organic Chemistry, University of Karlsruhe, Germany; 2Institute for Organic Chemistry, Budapest University of Technology and Economics, Hungary Elucidation of the 3D structure of histidine ammonia-lyase (HAL, EC 4.3.1.3) from Pseudomonas putida by X-ray crystallography revealed that the electrophilic prosthetic group at the active site is 3,5-dihydro-5-methylidene-4H-i´ midazol-4-one (MIO) [Schwede, T.F., Retey, J., Schulz, G.E. (1999) Biochemistry, 38, 5355 –5361].

9p system191 01-06-2013 52 3   Download

From the antennae of the moth Mamestra brassicae, we have identified a lepidopteran G protein a subunit belonging to the Gq family, through immunological detection in crude antennal extract and antennal primary cell cultures, followed by molecular cloning. The complete cDNA sequence (1540 bp) contains an open reading frame encoding a protein of 353 amino acids.

10p research12 01-06-2013 27 4   Download

The osmoregulated periplasmic glucans (OPGs) produced byRhodobacter sphaeroides, a free-living organism, were isolated by trichloracetic acid treatment and gel permeation chromatography. Compounds obtained were characterized by compositional analysis, matrix-assisted laser desorption ionization mass spectrometry and nuclear magnetic resonance.R. sphaeroidespredominantly synthesizes acyclic glucan containing 18 glucose residues that canbe substituted by one to seven succinyl esters residues at the C6position of some of the glucose residues, and by one or two acetyl resi-dues....

9p research12 29-04-2013 41 2   Download

Mammalian alcohol dehydrogenases (ADH) 3 form a com-plex enzyme system based on amino-acid sequence, func-tional properties, and gene expression pattern. At least four mouseAdhgenes are known to encode di€erent enzyme classes that share less than 60% amino-acid sequence iden-tity. Two ADH-containing and overlapping C57BL/6 bacterial arti®cial chromosome clones, RP23-393J8 and -463H24, were identi®ed in a library screen, physically mapped, and sequenced.

9p research12 29-04-2013 26 2   Download

OprM, the outer membrane component of the MexAB-OprM multidrug efflux pump ofPseudomonas aeruginosa, hasbeenassumed to facilitate the export of antibiotics across the outer membrane of this organism. Here we purified to homogeneity the OprM protein, reconstituted it into lipo-somemembranes, and tested its channel activitybyusing the liposome swellingassay. Itwas demonstrated thatOprMis a channel-forming protein and exhibits the channel property that amino acids diffuse more efficiently than saccharides....

8p research12 23-04-2013 43 4   Download

Hemolin, a plasma protein from lepidopteran insects, is composed of four immunoglobulin domains. Its synthesis is induced by microbial challenge. We investigated the biological functions of hemolin inManduca sexta.It was found to bind to the surface of bacteria and yeast, and caused these micro-organisms to aggregate. Hemolin was demonstrated to bind to lipopolysaccharide (LPS) from Gram-negativebacteriaand to lipoteichoic acid fromGram-positive bacteria.

8p research12 23-04-2013 39 2   Download

A nitroalkane-oxidizing enzyme gene (naoA) was cloned from a genomic DNA library ofStreptomyces ansochromo-genes7100. The deduced protein (NaoA) of this gene con-tains 363 amino acids and has high similarity to several nitroalkane-oxidizing enzymes from various micro-organ-isms.ThenaoAgenewas subcloned intoanexpressionvector pET23b and overexpressed inEscherichia coliBL21(DE3). The protein was then purified, and its characteristics were studied. Experimental results showed that NaoA can con-vert 1-nitropropane, 2-nitropropane and nitroethane into the corresponding carbonyl compounds....

6p tumor12 22-04-2013 44 2   Download

A new exocellular laccase was purified from the basidio-mycete C30. LAC2 is an acidic protein (pI¼3.2) prefer-entiallyproducedupona combined inductionby copper and p-hydroxybenzoate.The spectroscopic signature (UV/visible and EPR) of this isoform is typical of multicopper oxidases, but its enzymatic and physico-chemical properties proved to be markedly different from those of LAC1, the constitutive laccase previously purified from the same organism.

7p tumor12 22-04-2013 35 3   Download

Wehave identifiedanovelmammaliangene, termednicolin1 gene (NICN1), that is present in human, dog and mouse, whereas it is absent from the available genome sequences of nonmammalian organisms. TheNICN1gene consists of six exons and spans about 6 kb of genomic DNA. It encodes a 213 amino acid protein that does not belong to any known protein family. Experiments using green fluorescent protein (GFP)-taggednicolin1 fusionproteins indicate that nicolin1 is a nuclear protein.

6p tumor12 22-04-2013 53 2   Download

Nacre organic matrix has been conventionally classified as both
water-soluble and
water-insoluble,basedonits solubility in aqueous solutions after decalcificationwith acid or EDTA. Some characteristics (aspartic acid-rich, silk-fibroin-like content) were specifically attributed to either one or the other. The comparative study on the technique of extraction (extraction with water alone vs. demineralization with EDTA) presented here, seems to reveal that this gen-erally accepted classification may need to be reconsidered....

10p tumor12 22-04-2013 35 2   Download

Methyl-CpG-binding protein 2 (MeCP2) is a multifunc-tional protein involved in chromatin organization and silencing of methylated DNA. MAR-BD, a 125-amino-acid residue domain of chicken MeCP2 (cMeCP2, origin-ally named ARBP), is the minimal protein fragment required to recognize MAR elements and mouse satellite DNA. Here we report the solution structure of MAR-BD as determined by multidimensional heteronuclear NMR spectroscopy.

8p tumor12 20-04-2013 38 2   Download