Organic carbonyls

A nitroalkane-oxidizing enzyme gene (naoA) was cloned from a genomic DNA library ofStreptomyces ansochromo-genes7100. The deduced protein (NaoA) of this gene con-tains 363 amino acids and has high similarity to several nitroalkane-oxidizing enzymes from various micro-organ-isms.ThenaoAgenewas subcloned intoanexpressionvector pET23b and overexpressed inEscherichia coliBL21(DE3). The protein was then purified, and its characteristics were studied. Experimental results showed that NaoA can con-vert 1-nitropropane, 2-nitropropane and nitroethane into the corresponding carbonyl compounds....

6p tumor12 22-04-2013 44 2   Download

In organic chemistry, a carbonyl group is a functional group composed of a carbon atom double-bonded to an oxygen atom: C=O. It is common to several classes of organic compounds, as part of many larger functional groups. The term carbonyl can also refer to carbon monoxide as a ligand in an inorganic or organometallic complex. Other organic carbonyls are urea and the carbamates, the derivatives of acyl chlorides chloroformates and phosgene, carbonate esters, thioesters, lactones, lactams, hydroxamates, and isocyanates.

68p loc5heo 07-06-2011 122 14   Download