Origin of replication

Hypoxia interrupts the initiation of simian virus 40 (SV40) replication in vivo at a stage situated before unwinding of the origin region. After re-oxygenation, unwinding followed by a synchronous round of viral replication takes place. To further characterize the hypoxia-induced inhibition of unwinding, we analysed the binding of several replication proteins to the viral minichromosome before and after re-oxygenation.

11p research12 01-06-2013 41 4   Download

Mammalian mitochondria contain strong nuclease activity. Endonuclease G (endoG), which predominantly resides in mitochondria, accounts for a large part of this nuclease activity. It has been proposed to act as an RNase H-like nuclease on RNAÆDNA hybrids (R-loops) in the D-loop region where the origins of mitochondrial replication are mapped, providing RNA primers for mtDNA replication.

6p tumor12 22-04-2013 37 2   Download

Host species specificity of the polyomaviruses simian virus 40 (SV40) and mouse polyomavirus (PyV) has been shown to be determined by the host DNA polymerase a-primase complex involved in the initiation of both viral and host DNA replication. Here we demonstrate that DNA repli-cation of the related human pathogenic polyomavirus JC virus (JCV) can be supportedin vitro by DNA polymerase a-primase of either human or murine origin indicating that the mechanism of its strict species specificity differs from that of SV40 and PyV.

8p fptmusic 16-04-2013 41 3   Download

Replication of DNA is essential for the propagation of life. It is somewhat surprising then that, despite the vital nature of this process, cellular organ-isms show a great deal of variety in the mechanisms that they employ to ensure appropriate genome duplication. This diversity is manifested along classical evolutionary lines, with distinct combinations of replicon architec-ture and replication proteins being found in the three domains of life: the Bacteria, the Eukarya and the Archaea.

10p fptmusic 11-04-2013 69 3   Download

Licensing origins for replication upon completion of mitosis ensures genomic stability in cycling cells. Cdt1 was recently discovered as an essential licensing factor, which is inhibited by geminin. Over-expression of Cdt1 was shown to predis-pose cells for malignant transformation. We show here that Cdt1 is down-regulated at both the protein and RNA level when primary human fibroblasts exit the cell cycle into G0, and its expression is induced as cells re-enter the cell cycle, prior to S phase onset. ...

11p awards 05-04-2013 28 3   Download

Mastreviridae, of the family geminiviridae, possess a monopartite genome and are transmitted by leafhoppers. Bean yellow dwarf dirus (BeYDV) is a mastrevirus which originated from South Africa and infects dicoyledenous plants, a feature unusual for mastreviridae. Previously, the nonstructural proteins Rep and RepA were examined with respect to their independent roles in BeYDV replication.

13p inspiron33 25-03-2013 30 4   Download

We have developed a novel plasmid vector, pEBTetD, for full establish-ment of doxycycline-inducible protein expression by just a single transfec-tion. pEBTetD contains an Epstein–Barr virus origin of replication for stable and efficient episomal propagation in human cell lines, a cassette for continuous expression of the simple tetracycline repressor, and a cytomega-lovirus-type 2 tetracycline operator (tetO2)-tetO2 promoter.

8p galaxyss3 21-03-2013 38 3   Download

Human papillomavirus type 16 (HPV16) DNA replication, which requires two viral proteins E1 and E2, occurs only in the differentiating epithelium. Besides the general factors necessary for cellular DNA synthesis, other unidentified cellular factors are assumed to be involved in the regulation of HPV DNA replication.

13p galaxyss3 07-03-2013 40 2   Download

Replicative helicases are major motor proteins essential for chromosomal DNA replication in prokaryotes. Usually hexameric in solution, their DNA binding property must have different roles at stages ranging from the load-ing onto a branched structure at initiation from the origin to the highly pro-cessive translocation during elongation.

17p cosis54 08-12-2012 38 2   Download

Prediction of Saccharomyces cerevisiae replication origins Adam M Breier*, Sourav Chatterji† and Nicholas R Cozzarelli‡ Addresses: *Graduate Group in Biophysics, University of California-Berkeley, Berkeley, CA 94720-3204, USA. †Department of Computer Science, University of California-Berkeley, Berkeley, CA 94720-3204, USA. ‡Department of Molecular and Cellular Biology, Barker Hall, University of California-Berkeley, Berkeley, CA 94720-3204, USA. Correspondence: Nicholas R Cozzarelli. E-mail: ncozzare@socrates.berkeley.

0p thulanh21 15-11-2011 61 4   Download