Oxidoreductase

Đề tài nghiên cứu nhằm 2 mục tiêu: Tạo ra vector tái tổ hợp chứa đoạn gen mã hóa enzyme NQO1; thiết kế vector biểu hiện tạm thời thành công đoạn gen đó trong tế bào động vật HEK. Mời các bạn cùng tham khảo nội dung chi tiết.

78p closefriend03 13-10-2021 17 5   Download

Prolamellar bodies (PLB) contain two photochemically active forms of the enzyme protochlorophyllide oxidoreductase POR-PChlide640 and POR-PChlide650 (the spectral forms of POR-Chlide complexes with absorption maxima at the indicated wavelengths). Resuspension of maize PLB in media with a pH below 6.8 leads to a rapid conversion of POR-PChlide650 to POR-PChlide640 and a dramatic re-organization of the PLB membrane system. In the absence of excess NADPH, the absorption maximum of the POR complex undergoes a further shift to about 635 nm....

11p research12 01-06-2013 39 4   Download

Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. We have solved the crystal structure of the recombinant N-terminal catalytic domain (hTRXL-N) of ˚ hTRXL in its oxidized form at 2.2-A resolution.

9p research12 01-06-2013 41 4   Download

The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio alginolyticus was inactivated by reactive oxygen species. Highest Na+-NQR activity was observed in anaerobically prepared membranes that exhibited 1 : 1 coupling of NADH oxidation and Q reduction activities (1.6 UÆmg)1). Optical and EPR spectroscopy documented the presence of b-type cytochromes, a [2Fe)2S] cluster and an organic radical signal in anaerobically pre- pared membranes from V. alginolyticus.

6p research12 01-06-2013 46 4   Download

Protein molecular modelling and ligand docking were employed for the design of anthraquinone galactosyl-bio-mimetic dye ligands (galactosyl-mimodyes) for the target enzyme galactose dehydrogenase (GaDH). Using appro-priate modelling methodology, a GaDH model was build based on a glucose-fructose oxidoreductase (GFO) protein template.

15p tumor12 22-04-2013 46 2   Download

Tyrosinase (monophenol,L-DOPA:oxygen oxidoreductase) was isolated from the ink of the squid,Illex argentinus. Squid tyrosinase, termed ST94, was found to occur as a covalently linked homodimeric protein with a molecular mass of 140.2 kDa containing two copper atoms per a subunit. The tyrosinase activity of ST94 was enhanced by proteolysis with trypsin to form a protein, termed ST94t, with a molecular mass of 127.6 kDa.

13p tumor12 20-04-2013 30 3   Download

The plastid terminal oxidase (PTOX) encoded by theAra-bidopsis IMMUTANSgenewas expressed inEscherichia coli cells and its quinone/oxygen oxidoreductase activity moni-tored in isolated bacterial membranes using NADH as an electron donor. Specificity for plastoquinone was observed. Neither ubiquinone, duroquinone, phylloquinone nor ben-zoquinone could substitute for plastoquinone in this assay. However, duroquinol (fully reduced chemically) was an accepted substrate. Iron is also required and cannot be substituted by Cu 2+ ,Zn 2+ or Mn 2+ . ...

8p tumor12 20-04-2013 41 4   Download

The availability of a system for the functional expression of genes coding formolybdenumhydroxylases is a prerequisite for the construction of enzyme variants bymutagenesis. For the expression cloning of quinoline 2-oxidoreductase (Qor) fromPseudomonas putida86 – that contains the molybdo-pterin cytosine dinucleotide molybdenum cofactor (Mo-MCD), two distinct [2Fe)2S] clusters and FAD – the qorMSLgenes were inserted into the broad host range vector, pJB653, generating pUF1.P. putidaKT2440 and P. putida86-1Dqor were used as recipients for pUF1....

11p tumor12 20-04-2013 56 5   Download

Human novel reductase 1 (NR1) is an NADPH dependent diflavin oxidoreductase related to cytochrome P450reduc-tase (CPR). The FAD/NADPH- and FMN-binding domains of NR1 have been expressed and purified and their redox properties studied by stopped-flow and steady-state kinetic methods, and by potentiometry.

12p tumor12 20-04-2013 41 2   Download

The formation of disulfide bonds in the endoplasmic reti-culum requires protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin 1 (ERO1) that reoxi-dizes PDI. We report here that the expression of the rat, mouse andhumanhomologues ofERO1-Likeproteinabut not of the isoform ERO1-Lbare stimulated by hypoxia in rats vivo and in rat, mouse and human cell cultures.

8p fptmusic 16-04-2013 61 4   Download

Complex I (NADH:ubiquinone oxidoreductase) is the largest multiprotein enzyme of the oxidative phosphorylation system. Its assembly in human cells is poorly understood and no proteins assisting this process have yet been described. A good candidate is NDUFAF1, the human homologue of Neurospora crassacomplex I chaperone CIA30. Here, we demonstrate that NDUFAF1 is a mitochondrial protein that is involved in the complex I assembly process.

10p fptmusic 12-04-2013 46 4   Download

Ech hydrogenase fromMethanosarcina barkeriis a member of a distinct group of membrane-bound [NiFe] hydrogenases with sequence similarity to energy-conserving NADH:quinone oxidoreductase (complex I). The sequence of the enzyme predicts the binding of three [4Fe-4S] clusters, one by subunit EchC and two by subunit EchF. Previous studies had shown that two of these clusters could be fully reduced under 10 5 Pa of H2at pH 7 giving rise to two distinct S½ electron paramagnetic resonance (EPR) signals, designated as the g¼1.89 and theg¼1.92 signal....

13p fptmusic 12-04-2013 36 2   Download

Complex I (NADH: ubiquinone oxidoreductase) is the first complex in the respiratory electron transport chain. Homologs of this complex exist in bacteria, mitochondria and chloroplasts. The minimal complex I from mitochondria and bacteria contains 14 different subunits grouped into three modules: membrane, connecting, and soluble subcomplexes. The com-plex I homolog (NADH dehydrogenase or Ndh complex) from chloroplasts from higher plants contains genes for two out of three modules: the mem-brane and connecting subcomplexes. ...

12p awards 06-04-2013 26 3   Download

We foundN-methyl-l-amino acid dehydrogenase activity in various bacter-ial strains, such as Pseudomonas putidaandBacillus alvei, and cloned the gene fromP. putidaATCC12633 intoEscherichia coli. The enzyme purified to homogeneity from recombinant E. colicatalyzed the NADPH-dependent formation of N-alkyl-l-amino acids from the corresponding a-oxo acids (e.g. pyruvate, phenylpyruvate, and hydroxypyruvate) and alkylamines (e.g. methylamine, ethylamine, and propylamine).

7p awards 05-04-2013 42 3   Download

The ESSS protein is a recently identified subunit of mam-malian mitochondrial complex I. It is a relatively small integral membrane protein (122 amino acids) found in the b-subcomplex. Genomic sequence database searches reveal its localization to theX-chromosome inhumans andmouse. TheESSScDNAfromChinesehamster cellswas clonedand shown to complement one complementation group of our previously described mutants with a proposed X-linkage. Sequence analyses of the ESSS cDNA in these mutants revealed chain termination mutations. ...

9p awards 05-04-2013 35 5   Download

This work reports on the direct electrochemistry of the Desulfovibrio gigasaldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: two [2Fe-2S] centers and a molybdopterin cytosine dinucleotide cofactor. The voltammetric behavior of the enzyme was analyzed at gold and carbon (pyrolytic graphite and glassy carbon) elec-trodes. Two different strategies were used: one with the molecules confined to the electrode surface and a second withDgAOR in solution....

10p dell39 03-04-2013 41 4   Download

Aldehyde oxidoreductase ofEubacterium acidaminophilum was purified to homogeneity under strict anaerobic condi-tions using a four-step procedure. The purified enzyme was present as a monomer with an apparent molecular mass of 67 kDa and contained 6.0± 0.1 iron, 1.1 ± 0.2 tungsten, about 0.6 mol pterincofactor andzinc, but nomolybdenum. The enzymeactivitywas induced if amolar excessof electron donors, such as serine and/or formate, were supplied in the growth medium compared to readily available electron acceptors such as glycine betaine....

8p dell39 03-04-2013 38 4   Download

Protein disulfide oxidoreductases are ubiquitous redox enzymes that catalyse dithiol–disulfide exchange reactions with a CXXC sequence motif at their active site. Adisulfide oxidoreductase, a highly thermostable protein, was isolated from Pyrococcus furiosus(PfPDO), which is characterized by two redox sites (CXXC) and an unusual molecularmass. Its 3D structure at high resolution suggests that it may be related to the multidomain protein disulfide-isomerase (PDI), which is currently known only in eukaryotes....

12p dell39 03-04-2013 35 3   Download

The last step of the biosynthesis ofl-ascorbic acid (vitamin C) in plants and animals is catalyzed by l-gulono-1,4-lactone oxidoreductases, which use bothl-gulono-1,4-lactone and l-galactono-1,4-lactone as substrates. l-Gul-ono-1,4-lactone oxidase is missing in scurvy-prone, vitamin C-deficient ani-mals, such as humans and guinea pigs, which are also highly susceptible to tuberculosis.

11p inspiron33 25-03-2013 52 4   Download

Disulfide bonds are required for the stability and function of a large num-ber of proteins. Recently, the results from genome analysis have suggested an important role for disulfide bonds concerning the structural stabilization of intracellular proteins from hyperthermophilic Archaea and Bacteria, contrary to the conventional view that structural disulfide bonds are rare in proteins from Archaea.

16p inspiron33 25-03-2013 36 5   Download