Paracoccus

Luận án Tiến sĩ Hóa học "Nghiên cứu tối ưu quy trình tạo chế phẩm giàu canthaxanthin từ vi khuẩn ưa mặn Paracoccus carotinifaciens VTP20181 và bước đầu ứng dụng trong chăn nuôi cá hồi vân" trình bày nghiên cứu, tối ưu hóa quá trình lên men thu sinh khối giàu canthaxanthin từ vi khuẩn ưa mặn; Nghiên cứu tối ưu hóa quá trình chiết xuất canthaxanthin từ sinh khối vi khuẩn ưa mặn; Tổng hợp liposome có chứa canthxanthin và ứng dụng bổ sung vào thức ăn nhằm nâng cao chất lượng và mầu sắc thịt cá hồi vân (Oncorhynchus mykiss).

133p vineville 03-02-2023 22 9   Download

Đề tài đi sâu nghiên cứu thành phần loài rệp sáp, đặc điểm sinh vật học, sinh thái của loài rệp sáp giả Paracoccus marginatus hại cây đu đủ (trong Luận án này gọi tắt là rệp sáp giả P. marginatus hay rệp sáp giả hại đu đủ) và hiệu quả của các biện pháp phòng chống rệp sáp giả P. marginatus trên cây đu đủ. Trên cơ sở đó, xây dựng và đề xuất các biện pháp phòng chống rệp sáp giả P. marginatus đạt hiệu quả cao, đáp ứng yêu cầu kinh tế, thân thiện với môi trường tại một số vùng trồng đu đủ ở Hà Nội.

168p chuheodethuong 09-07-2021 49 9   Download

Introducing site-directed mutations in surface-exposed residues of subunit II of the heme aa3 cytochrome c oxidase of Paracoccus denitrificans, we analyze the kinetic parameters of electron transfer from reduced horse heart cytochrome c. Specifically we address the following issues: (a) which residues on oxidase contribute to the docking site for cytochrome c, (b) is an aromatic side chain required for electron entry from cytochrome c, and (c) what is the molecular basis for the previously observed biphasic reaction kinetics....

9p system191 01-06-2013 37 3   Download

Tightly coupled inside-out vesicles were prepared from Paracoccus denitrificanscells (SPP, sub-Paracoccusparticles) and characterized kinetically. The rate of NADHoxidation, catalysed by SPP, increases 6–8 times on addition of gram-icidin. The vesicles are capable of catalysing DlH + -dependent reverse electron transfer fromquinol toNAD + . The kinetic parameters of the NADH-oxidase and the reverse electron transfer carried out by membrane-bound P. denitrificanscomplex I were estimated and compared with those of the mitochondrial enzyme. ...

5p research12 23-04-2013 43 3   Download

The structure of cytochromec-550 from the nonphotosynthetic bacteria Paraccocus versutushas been solved by X-ray crystallography to 1.90 A˚ resolution, and reveals a high structural homology to other bacterial cyto-chromesc2. The effect of replacing the axial heme-iron methionine ligand with a lysine residue on protein structure and unfolding has been assessed using the M100K variant.

15p awards 06-04-2013 40 3   Download

In recent studies on heme-copper oxidases a particular glutamate residue in subunit II has been suggested to constitute the entry point of the so-called K pathway. In contrast, mutations of this residue (E78 II ) in the Paracoccus denitrificanscytochromecoxidase do not affect its catalytic activity at all (E78 II Q) or reduce it to about 50% (E78 II A); in the latter case, the mutation causes no drastic decrease in hemea3 reduction kinetics under anaerobic con-ditions, when compared to typical K pathway mutants....

9p awards 05-04-2013 29 4   Download

Two soluble enzymes (FerA and FerB) catalyzing the reduction of a number of iron(III) complexes by NADH, were purified to near homogeneity from the aerobically grown iron-limited culture ofParacoccus denitrificansusing a combination of anion-exchange chromatography (Seph-arose Q), chromatofocusing (Mono P), and gel permeation chromatography (Superose 12). FerA is a monomer with a molecular mass of 19 kDa, whereas FerB exhibited a molecular mass of about 55 kDa and consists of probably two identical subunits. ...

10p dell39 03-04-2013 41 2   Download

The heterologous expression of tryptophan trytophylquinone (TTQ)-dependent aromatic amine dehydrogenase (AADH) has been achieved in Paracoccus denitrificans. The aauBEDAgenes andorf-2from the aromatic amine utilization (aau) gene cluster of Alcaligenes faecaliswere placed under the regulatory control of themauFpromoter fromP. denitrificans and introduced intoP. denitrificansusing a broad-host-range vector.

16p dell39 27-03-2013 42 4   Download

The protonation state of residues around the Qo binding site of the cyto-chrome bc1 complex from Paracoccus denitrificansand their interaction with bound quinone(s) was studied by a combined electrochemical and FTIR difference spectroscopic approach. Site-directed mutations of two groups of conserved residues were investigated: (a) acidic side chains located close to the surface and thought to participate in a water chain leading up to the heme bLedge, and (b) residues located in the vicinity of this site. ...

13p inspiron33 23-03-2013 41 5   Download

The Rieske [2Fe)2S] protein (ISP) is an essential subunit of cyto-chromebc1 complexes in mitochondrial and bacterial respiratory chains. Based on the presence of two consecutive arginines, it was argued that the ISP ofParacoccus denitrificans, a Gram-negative soil bacterium, is inserted into the cytoplasmic membrane via the twin-arginine translocation (Tat) pathway.

14p inspiron33 23-03-2013 39 3   Download

Biogenesis of cytochromec oxidase (COX) is a highly complex process involving 30 chaperones in eukaryotes; those required for the incorpora-tion of the copper and heme cofactors are also conserved in bacteria. Surf1, associated with hemeainsertion and with Leigh syndrome if defec-tive in humans, is present as two homologs in the soil bacterium Paracoc-cus denitrificans, Surf1c and Surf1q.

10p cosis54 05-01-2013 40 4   Download