PCR assays

We aimed to screen novel gene signatures for ovarian cancer (OC) and explore the role of biomarkers in OC via regulating pyroptosis using bioinformatics analysis. The proliferation, migration, and invasion were determined using cell counting kit and transwell assay. Enzyme-linked immunosorbent assay was applied to measure infammatory factors. The mRNA and protein expression were detected using real-time quantitative PCR and western blot.

15p vishanshan 27-06-2024 1 1   Download

This study was conducted to determine the composition of Toxocara species in dogs and cats in Vietnam. One hundred and twenty-seven Toxocara adult worms were collected from dogs and cats and were analyzed by PCR-RFLP assay and DNA sequencing of a partial section of the cox1 gene.

7p vigojek 02-02-2024 2 0   Download

Ebook "Early, rapid and sensitive veterinary molecular diagnostics - Real time PCR applications" gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system.

319p tachieuhoa 28-01-2024 4 2   Download

Ebook "PCR for clinical microbiology: An Australian and international perspective" provides detailed and easy step by step protocols for diagnostic assays in the field of molecular microbiology; provides a valuable overview of molecular diagnostics in infectious diseases; medical criteria for choice of molecular test; guide to set up a clinical molecular diagnostic laboratory;...

420p tachieuhoa 28-01-2024 3 2   Download

This study was conducted to determine the composition of Toxocara species in dogs and cats in Vietnam. One hundred and twenty-seven Toxocara adult worms were collected from dogs and cats and were analyzed by PCR-RFLP assay and DNA sequencing of a partial section of the cox1 gene.

7p viplato 02-01-2024 2 0   Download

Ebook "Genome editing in animals: Methods and protocols" details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal.

258p lucchinguyen 28-12-2023 4 2   Download

In this study, we found that LINC00852 was highly expressed in the tissue of prostate cancer using quantitative reverse transcription PCR (qRT-PCR). CCK-8 assay, colony formation experiment, Transwell migration and invasion experiments were performed to prove that the up-regulation of LINC00852 could promote the proliferation, migration and invasion of prostate cancer cells in vitro.

13p vileonardodavinci 23-12-2023 4 2   Download

To investigate the potential role of Long Non-coding RNAs (lncRNAs) in the progression of osteosarcoma. Methods: The candidate lncRNAs were screened with RNA-seq and confirmed with quantitative real-time PCR. Using MTS, transwell assay, and fow cytometric analysis, the effects of overexpressed lnc-SELPLG-2:1 on cell functions were determined.

15p vialfrednobel 23-12-2023 6 3   Download

Part 2 book "Protocols for the diagnosis of pig viral diseases" includes content: Lateral flow assay for diagnosis of pig viral diseases; droplet digital PCR based diagnosis for porcine viral diseases; protocols for immunofluorescence techniques; polymerase spiral reaction (PSR) for the diagnosis of porcine viral diseases; recombinase polymerase amplification based diagnostics of porcine viral diseases; cell culture system for porcine virus isolation and propagation,... and other contents.

180p muasambanhan02 18-12-2023 5 4   Download

Part 2 book "Animal influenza virus" includes content: A brief introduction to influenza a virus in swine; sample types, collection, and transport for influenza a viruses of swine; isolation of swine influenza virus in cell cultures and embryonated chicken eggs; RNA extraction from swine samples and detection of influenza a virus in swine by real time RT PCR; hemagglutinin inhibition assay with swine sera; enzyme linked immunosorbent assay for detection of serum or mucosal isotype specific igg and iga whole ­virus antibody to influenza a virus in swine,.... and other contents.

175p oursky10 07-12-2023 5 2   Download

As clinical signs and symptoms of nasopharyngeal carcinoma (NPC) are often nonspecific, it is difficult to detect in the early stage, leading to high risk of relapse or recurrent metastasis. Plasma cell-free DNA of Epstein-Barr virus (cf-EBV DNA) is markedly associated with the development of undifferentiated NPC, and its level prior and subsequent to treatment is reliable for the screening, diagnosis, monitoring and prognosis of NPC.

5p visoros 01-11-2023 3 1   Download

To investigate how the negative regulation of CD44st by miR-138-5p affects the invasive ability of breast cancer cell lines and prognosis in postoperative breast cancer patients. RT-PCR, qRT-PCR, and western blot assays were used to detect the expression of CD44s, CD44v6, and CD44st at both mRNA and protein levels. The expression of miR-138-5p in breast cancer cell lines was also evaluated.

14p vischultz 20-10-2023 1 1   Download

Part 1 book "Molecular diagnostic PCR handbook" includes content: Background; PCR – the basic reaction; amplicon detection, analysis and PCR evaluation; limitations and troubleshooting, specific types of PCR assays.

118p oursky06 17-10-2023 5 1   Download

Part 2 book "Molecular diagnostic PCR handbook" includes content: The PCR laboratory, disease diagnosis using PCR specific procedures for important veterinary pathogens, the detection of Mycoplasma, contagious bovine pleuropneumonia and contagious caprine pleuropneumonia, using a PCR assay, the detection of Rift valley fever virus using a PCR assay, the detection of lumpy skin disease virus using a PCR assay.

207p oursky06 17-10-2023 5 1   Download

Part 1 book "PCR Technology - Current innovations" includes content: Pre-PCR processing strategies, use of a liposomal internal control vehicle for whole process quality assurance of nucleic acid amplification, using scorpion primers for genotyping, thermocycler calibration and analytical assay validation, dropletization of bioreactions - how single cell quantitative analyses may improve gene measurement and cancer diagnosis,.... and other contents.

192p oursky06 17-10-2023 3 1   Download

Part 2 book "PCR Technology - Current innovations" includes content: Assay design for Real-Time qPCR, primer design for large scale multiplex PCR and arrayed primer extension, hypothesis driven approaches to multivariate analysis of qPCR data, Experiment Design, data management, and univariate statistical analysis of gene expression data obtained by real time quantitative PCR,.... and other contents.

261p oursky06 17-10-2023 3 1   Download

Part 2 book "Quantitative real-time PCR - Methods and protocols" includes content: Real-Time PCR detection of mycoplasma pneumoniae in the diagnosis of community acquired pneumonia, a sensible technique to detect mollicutes impurities in human cells cultured in GMP condition, real time quantification assay to monitor BCR ABl1 transcripts in chronic myeloid leukemia, a reliable assay for rapidly defining transplacental metastasis using quantitative PCR,... and other contents.

129p oursky05 20-09-2023 5 1   Download

Part 1 book "ELISA - Methods and protocols" includes content: Flow through assay for detection of antibodies using protein a colloidal gold conjugate as a probe; multiplexed microsphere suspension array based immunoassays; lateral flow immunoassay, immuno pcr assay for sensitive detection of proteins in real time; tyramide signal amplification for immunofluorescent enhancement;.... and other contents.

116p oursky05 20-09-2023 2 2   Download

Outbreaks of the Southern rice black-streaked dwarf virus (SRBSDV) have caused significant losses in many rice-growing areas in Vietnam, especially in both North and Central Vietnam in recent years. To detect the virus, traditional reverse transcription polymerase chain reaction (RT-PCR) methodology and immunoassays are currently employed. RT-PCR is accurate but requires expensive chemicals and instruments, as well as complex procedures that limit its applicability for field tests.

8p viengels 25-08-2023 5 2   Download

To develop a method using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) to genotype MTHFR rs1801133 polymorphism in Vietnamese people. Methods: Fifteen DNA samples from a group of Vietnamese people were used to evaluate this assay. The Sanger sequencing method was used to identify the standard genotypes of the rs1801133 polymorphism.

7p viwhitewolf 11-07-2023 1 1   Download