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PCR evaluation
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The addition of pertuzumab (P) to trastuzumab (H) and standard chemotherapy (CT) as neoadjuvant treatment (NaT) for patients with HER2+breast cancer (BC), has shown to increase the pathological complete response (pCR) rate, without main safety concerns. The aim of NeoPowER trial is to evaluate safety and efficacy of P+H+CT in a real–world population.
18p
vikoch
27-06-2024
1
1
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To inititally evaluate the efficiency of cryoprotectant-free vitrification in PCR tubes on a low number of human spermatozoa. Methods: A laboratory experimental study was conducted on 30 severe oligospermia treated at the Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University.
8p
vijaychest
16-05-2024
2
1
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. In this study, 65-bp indel polymorphism in the GOLGB1gene was evaluated in association with body weight in Noi chickens. The live body weight of 170 chickens (90 females, 80 males) at continuous ages of 7-day intervals (from 28 to 84 days) was recorded. A 65-bp indel polymorphism in the GOLGB1 gene was analyzed using the PCR method.
8p
dianmotminh02
03-05-2024
3
1
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The deregulation of microRNA-140 (miRNA-140) has been reported to be involved in the human pathogenesis of Osteoarthritis (OA). However, in Vietnam, to our knowledge, there is a limited study of evaluation of a miRNA-140 expression. The commercial kits for the quantitation of miRNA-140 are more expensive.
7p
vibego
02-02-2024
1
0
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The modified systemic inflammation score (mSIS) system, which is constructed based on the neutrophil to lymphocyte ratio (NLR) and albumin (Alb), has not been applied to evaluate the prognosis of malignant breast cancer patients who underwent neoadjuvant chemotherapy (NAC). The present study aimed to explore the relationship between the mSIS and overall survival (OS), disease-free survival (DFS) and pathological complete response (pCR).
15p
vileonardodavinci
23-12-2023
8
3
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In recent years, it has been proved that necroptosis plays an important role in the occurrence, development, invasion, metastasis and drug resistance of malignant tumors. Hence, further evaluation and targeting of necroptosis may be of clinical benefit for gynecologic cancers (GCs).
21p
vileonardodavinci
23-12-2023
5
3
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To investigate how the negative regulation of CD44st by miR-138-5p affects the invasive ability of breast cancer cell lines and prognosis in postoperative breast cancer patients. RT-PCR, qRT-PCR, and western blot assays were used to detect the expression of CD44s, CD44v6, and CD44st at both mRNA and protein levels. The expression of miR-138-5p in breast cancer cell lines was also evaluated.
14p
vischultz
20-10-2023
1
1
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Part 1 book "Molecular diagnostic PCR handbook" includes content: Background; PCR – the basic reaction; amplicon detection, analysis and PCR evaluation; limitations and troubleshooting, specific types of PCR assays.
118p
oursky06
17-10-2023
5
1
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Part 1 book "Vaccine design, methods and protocols (Vol 1 - Vaccines for human diseases)" includes content: Clinical impact of vaccine development, future challenges for vaccinologists, principles of vaccination, proteomic monitoring of b-cell immunity, application of droplet digital PCR to validate rift valley fever vaccines, methods to evaluate novel hepatitis C virus vaccines, designing efficacious vesicular stomatitis virus vectored vaccines against ebola virus,.... and other contents.
491p
oursky05
20-09-2023
0
0
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To develop a method using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) to genotype MTHFR rs1801133 polymorphism in Vietnamese people. Methods: Fifteen DNA samples from a group of Vietnamese people were used to evaluate this assay. The Sanger sequencing method was used to identify the standard genotypes of the rs1801133 polymorphism.
7p
viwhitewolf
11-07-2023
1
1
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The evaluation and interpretation of forensic DNA mixture evidence faces greater interpretational challenges due to increasingly complex mixture evidence. Such challenges include: casework involving low quantity or degraded evidence leading to allele and locus dropout; allele sharing of contributors leading to allele stacking; and differentiation of PCR stutter artifacts from true alleles.
15p
vinarcissa
21-03-2023
5
1
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fter Salmonella strains were isolated from food samples collected randomly at conventional markets in several districts in Ho Chi Minh city, we evaluated antibiotic resistance by Kirby-Bauer methods, and the serotype name was assigned according to ISO/TR 6579-3:2014 and class 1 integron was investigated by PCR technique.
11p
viporsche
03-11-2022
16
5
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To evaluate the feasibility and performance of self-collected vaginal swab samples for HPV screening among women in Lagos, Nigeria. With high accuracy of 96%, self-collected sampling is adequate when tested with real-time PCR and may increase the uptake of HPV testing.
7p
viferrari
29-10-2022
5
2
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Nucleic acid amplification technologies (NAAT) are advancing our ability to make rapid molecular diagnoses in patients with serious culture negative infections. This is the first report of PCR coupled to electrospray ionization mass spectrometry use in the evaluation of complicated community acquired pneumonia in a pediatric patient.
5p
viisaacnewton
26-04-2022
16
2
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African swine fever (ASF) is the most important disease to the pigs and cause serious economic losses to the countries with large-scale swine production. Vaccines are recognized as the most useful tool to prevent and control ASF virus (ASFV) infection.
9p
vigandhi
23-02-2022
19
1
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Campylobacter fetus subsp. venerealis (Cfv) is the pathogen responsible for Bovine Genital Campylobacteriosis (BGC), a venereal disease of cattle associated with impaired reproductive performance. Although several PCR assays were developed to identify this pathogen, most of them are still poorly evaluated in clinical samples.
9p
vigandhi
23-02-2022
8
1
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Real time RT-PCR (qPCR) is a useful and powerful tool for quantitative measurement of gene expression. The proper choice of internal standards such as reference genes is crucial for correct data evaluation. In female dogs, as in other species, the reproductive tract is continuously undergoing hormonal and cycle stagedependent morphological changes, which are associated with altered gene expression.
12p
vigandhi
23-02-2022
9
1
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Ureaplasma diversum has numerous virulence factors that contribute to pathogenesis in cattle, including Lipid-associated membrane proteins (LAMPs). Therefore, the objectives of this study were to evaluate in silico important characteristics for immunobiological applications and for heterologous expression of 36 LAMPs of U. diversum (UdLAMPs) and, also, to verify by conventional PCR the distribution of these antigens in strains of Brazilian states (Bahia, Minas Gerais, São Paulo, and Mato Grosso do Sul).
16p
vigandhi
23-02-2022
8
1
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The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G2 transgenic chickens to evaluate suppression of viral transmission.
6p
vigandhi
23-02-2022
8
1
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This study aimed to screen the sera of goats and sheep from flocks suspected of Mycobacterium avium subsp. paratuberculosis (MAP) infection by a newly standardized Mce-truncated ELISA (Mt-ELISA) kit for the detection of antibodies against MAP. Four diagnostic applied tests were evaluated including Indigenous plate-ELISA (IP-ELISA), Mt-ELISA, fecal Polymerase Chain Reaction (f-PCR) and fecal culture (FC).
9p
vidarwin
22-02-2022
9
1
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